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Abstract(s)
Tuberaria lignosa (Sweet) Samp. (“alcária”), a plant mostly found in Western regions
of the Iberian Peninsula, has antioxidant properties due to its composition in ascorbic
acid and phenolic compounds [1]. Given these antioxidant properties, together with the
traditional use of the plant to treat several diseases, the aims of this work were to: i)
investigate if aqueous extracts from this plant, obtained by infusion and decoction, were
inhibitors of cell growth in three human tumor cell lines; ii) study the cellular
mechanism of action of the most potent extract.
A screening of tumor cell growth inhibitory potential was performed with the
Sulforhodamine B (SRB) assay using three different human tumor cell lines: MCF-7
(breast adenocarcinoma), NCI-H460 (non-small cell lung cancer) and HCT-15
(colorectal adenocarcinoma). Results showed that both aqueous extracts of T. lignosa
decreased the growth of the cell lines tested and that the T. lignosa infusion extract was
the most potent one, particularly in the NCI-H460 and HCT-15 cells.
The T. lignosa infusion extract was further tested in the NCI-H460 cells. A
determination of its effect on cell cycle profile was carried out, by analyzing cellular
DNA content by flow cytometry following incubation with propidium iodide.
Determination of cellular apoptosis was also performed, with the Annexin V-FICT and
propidium iodide assay, and analyzed by flow cytometry.
Preliminary results showed that the selected extract promoted a slight increase in the
percentage of cells in the G1 phase of the cell cycle and induced cellular apoptosis.
In conclusion, the T. lignosa extract decreased growth of the human tumor cell lines
tested and the most potent effect was observed for the T. lignosa infusion extract. Future
work will confirm if this effect is due mainly to induction of apoptosis.
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Citation
Pereira, J.; Rodrigues, V.L.; Lima, M.J.; Lima, Raquel T.; Ferreira, Isabel C.F.R.; Vasconcelos, M. Helena (2015). Study of the cell growth inhibitory effect of aqueous extracts from Tuberaria lignosa in human tumor cell lines. In 8th Meeting of Young Researchers of University of Porto