Repository logo
 
Publication

Valorisation of a threatened plant species through in vitro culture elicitation of phenolic compounds

2019Conference object Open access
Simple item page
dc.contributor.authorAyuso, Manuel
dc.contributor.authorDias, Maria Inês
dc.contributor.authorPinela, José
dc.contributor.authorGallego, Pedro Pablo
dc.contributor.authorBarreal, M. Esther
dc.contributor.authorBarros, Lillian
dc.contributor.authorFerreira, Isabel C.F.R.
dc.date.accessioned2019-06-26T09:30:22Z
dc.date.available2019-06-26T09:30:22Z
dc.date.issued2019
dc.description.abstractAbstract: Ex situ conservation strategies, such as in vitro culture, have been suggested for the recovery of threatened plants close to extinction. In vitro culture or micropropagation is a plant-multiplication technique that allows to obtain a large number of clones in a short period of time and limited space, which can be used in reintroduction programs and for research purposes (1). This biotechnological tool also allows increasing the production yield of secondary metabolites, such as phenolic compounds, thus emerging as an efficient system to obtain high added-value bioactive compounds (2). This approach allows valorising the culture of threatened plants for their commercial use in different industrial sectors interested in bioactive compounds, such as the food and pharmaceutical industries. Therefore, the aim of this research was to study the phenolic compounds elicitation by cytokinins in roots and aerial parts of the in vitro cultured endangered plant Eryngium viviparum J. Gay (Fam. Apiaceae). E. viviparum explants were cultured in vitro in a solidified MS medium. A 3-level factorial design was implemented to study the effect of two cytokinins, 6-benzylaminopurin (BAP) and kinetin (KIN), at 0, 1 and 2 mg L-1, on the elicitation of phenolic compounds. The obtained plant material was lyophilized, reduced to powder, and submitted to a solid-liquid extraction with ethanol:water (80:20, v/v) to obtain combined extracts (3). Then, phenolic compounds were identified and quantified by HPLC-DAD-ESI/MS (3) and the main phenolic compounds with known bioactivities were correlated with both cytokinins. Seventeen phenolic compounds were identified in all E. viviparum hydroethanolic extracts, corresponding to 12 phenolic acids and 5 flavonoids. Rosmarinic and chlorogenic acids were the main bioactive phenolic acids. Regarding flavonoids, tectorigenin-O-glucuronide was identified and quantified only in the aerial parts. In the roots, the increase of phenolic acids was correlated with the presence of KIN in the culture media. Concerning the aerial parts, cytokinins did not correlate with phenolic acids, but KIN correlated with the increase of tectorigenin-O-glucuronide. In addition, the roots had a higher phenolic composition content than the aerial parts and the combination of both cytokinins promoted a doubling of the phenolic compounds concentration in some samples. Therefore, this study demonstrated the efficacy of in vitro culture in adding value to the threatened plant species E. viviparum, as the levels of valuable secondary metabolites were increased by elicitation, thus being a sustainable approach that can help save and valorise other endangered plants.pt_PT
dc.description.sponsorshipTo FCT (Portugal) and FEDER under programme PT2020 for financial support to CIMO (UID/AGR/00690/2019) and the research contracts of J. Pinela (Project AllNatt, POCI-01-0145- FEDER-030463), M.I Dias and L. Barros. To FEDER-Interreg España- Portugal programme for financial support through the project 0377_Iberphenol_6_E. To IACOBUS research stay program for the financial support of M. Ayuso.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAyuso, Manuel; Dias, Maria Inês; Pinela, José; Gallego, Pedro Pablo; Barreal, M. Esther; Barros, Lillian; Ferreira, Isabel C.F.R. (2019). Valorisation of a threatened plant species through in vitro culture elicitation of phenolic compounds. In Congreso Nacional de Biotecnología. Vigopt_PT
dc.identifier.urihttp://hdl.handle.net/10198/19361
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationMountain Research Centre
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectFood biotechnologypt_PT
dc.subjectPhenolic compoundspt_PT
dc.titleValorisation of a threatened plant species through in vitro culture elicitation of phenolic compoundspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleMountain Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FAGR%2F00690%2F2019/PT
oaire.citation.conferencePlaceVigo, Espanhapt_PT
oaire.citation.titleBIOTEC 2019 – Congresso Nacional de Biotecnologiapt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameAyuso
person.familyNameDias
person.familyNamePinela
person.familyNameBarros
person.familyNameFerreira
person.givenNameManuel
person.givenNameMaria Inês
person.givenNameJosé
person.givenNameLillian
person.givenNameIsabel C.F.R.
person.identifierABC-7764-2020
person.identifier469085
person.identifier144781
person.identifier.ciencia-id6511-A057-C55F
person.identifier.ciencia-id2A13-4BE6-C7CF
person.identifier.ciencia-id771C-2B43-B108
person.identifier.ciencia-id9616-35CB-D001
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0002-1697-3857
person.identifier.orcid0000-0001-8744-7814
person.identifier.orcid0000-0001-7523-1637
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridM-8242-2013
person.identifier.ridB-4466-2014
person.identifier.ridJ-3600-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id54388787000
person.identifier.scopus-author-id54392272800
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationb79f5829-1b7c-485f-8682-24e0b437c550
relation.isAuthorOfPublication7ca8432b-e7f4-4116-a56e-9eba0d4403bd
relation.isAuthorOfPublication706cd9ff-065b-4c98-98a7-5c0a9e45adc4
relation.isAuthorOfPublication3af07ffe-f914-48ba-a5d5-efcf70fdce01
relation.isAuthorOfPublicationbd0d1537-2e03-41fb-b27a-140af9c35db8
relation.isAuthorOfPublication.latestForDiscoverybd0d1537-2e03-41fb-b27a-140af9c35db8
relation.isProjectOfPublicationd6683ba1-d253-48e4-968e-35106ce7b750
relation.isProjectOfPublication.latestForDiscoveryd6683ba1-d253-48e4-968e-35106ce7b750

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Poster Int. 296.pdf
Size:
3.14 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CIMO - Resumos em Proceedings Não Indexados à WoS/Scopus