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Tumour cell growth inhibitory potential of mushroom extracts from the genus Suillus

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dc.contributor.authorVaz, Josiana A.
dc.contributor.authorSantos, Tiago
dc.contributor.authorTavares, Catarina
dc.contributor.authorCalhelha, Ricardo C.
dc.contributor.authorMartins, Anabela
dc.contributor.authorAlmeida, Gabriela M.
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorVasconcelos, M. Helena
dc.date.accessioned2013-02-27T15:24:04Z
dc.date.available2013-02-27T15:24:04Z
dc.date.issued2012
dc.description.abstractMushrooms are a source of compounds with promising antitumour activity [1]. We have been working on the identification of wild mushrooms with promising antitumour activity and a Clitocybe alexandri extract which induces cell cycle arrest and apoptosis in a lung cancer cell line has been previously identified by part of the team [2]. The objective of this work was to continue the identification of mushrooms from the Northeast of Portugal with tumour cell growth inhibitory potential. Thirty six wild edible mushrooms were collected and taxonomically identified. Various extracts were prepared and screened for growth inhibitory potential with the SRB assay, in four human tumour cell lines: MCF-7 (breast), NCI-H460 (NSCLC), AGS (gastric) and HCT-15 (colon). These screens allowed the identification of two Suillus species whose methanolic extracts presented potent activity: S. collinitus and S. luteus. The S. collinitus extract was more potent in the MCF-7 cells (GI50=25.2±0.16 µg/ml), causing G1 cell cycle arrest and increasing apoptosis. An increase in p53 and p21 was verified, suggesting that the effect was p53-mediated [3]. The S. luteus extract was slightly more potent in the HCT-15 cells (with mutant p53, GI50=17.8 ± 1.6 µg/ml) than in the other cell lines tested, indicating that its effect was not p53-dependent. In fact, it increased the levels of p53 but the alterations in proteins transactivated by p53 (e.g. Bax) were not consistent with a p53-mediated effect. Growth inhibition in the HCT-15 cells was mostly due to inhibition of cell proliferation and cell cycle arrest in G1, rather than induction of cell death. Finally, this extract had no effect in primary cultures of porcine hepatocytes (GI50>400 µg/ml). In conclusion, these two Suillus species present promising tumour cell growth inhibitory effect and the S. luteus extract may be particularly interesting considering that most tumours present mutant p53.por
dc.description.sponsorshipFCT and COMPETE/QREN/EU- project PTDC/AGR-ALI/110062/2009. They also thank to University of Porto and Santander Totta for financial support. FCT, POPH-QREN and ESF for grants to JAV (BD/43653/2008) and RCC (BPD/68344/2010). GMA is supported by FCT and ESF.por
dc.identifier.citationVaz, Josiana A.; Santos, Tiago; Tavares, Catarina; Calhelha, Ricardo C.; Martins, Anabela; Almeida, Gabriela; Ferreira, Isabel C.F.R.; Vasconcelos, M. Helena (2012). Tumour cell growth inhibitory potential of mushroom extracts from the genus Suillus. In 3º Encontro Nacional de Química Terapêutica e 1st Portuguese-Spanish-Brazilian Meeting on Medicinal Chemistry. Aveiropor
dc.identifier.urihttp://hdl.handle.net/10198/8191
dc.language.isoengpor
dc.peerreviewedyespor
dc.relationPortuguese Wild Mushrooms: Chemical characterization and functional study of antiproliferative and proapoptotic properties in cancer cell lines
dc.relationPOTENCIAL NUTRACEUTICO DE COGUMELOS SILVESTRES DO NORDESTE DE PORTUGAL: ESTUDO DE PROPRIEDADES ANTIO-XIDANTES, ANTIPROLIFERATIVAS E INDUTORAS DA APOPTOSE
dc.titleTumour cell growth inhibitory potential of mushroom extracts from the genus Suilluspor
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitlePortuguese Wild Mushrooms: Chemical characterization and functional study of antiproliferative and proapoptotic properties in cancer cell lines
oaire.awardTitlePOTENCIAL NUTRACEUTICO DE COGUMELOS SILVESTRES DO NORDESTE DE PORTUGAL: ESTUDO DE PROPRIEDADES ANTIO-XIDANTES, ANTIPROLIFERATIVAS E INDUTORAS DA APOPTOSE
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FAGR-ALI%2F110062%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F43653%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F68344%2F2010/PT
oaire.citation.conferencePlaceAveiropor
oaire.citation.title3º Encontro Nacional de Química Terapêutica e 1st Portuguese-Spanish-Brazilian Meeting on Medicinal Chemistry, 28 a 30 de Novembropor
oaire.fundingStream5876-PPCDTI
oaire.fundingStreamSFRH
person.familyNameVaz
person.familyNameCalhelha
person.familyNameMartins
person.familyNameFerreira
person.givenNameJosiana A.
person.givenNameRicardo C.
person.givenNameAnabela
person.givenNameIsabel C.F.R.
person.identifier144781
person.identifier.ciencia-id171B-88BA-64F5
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person.identifier.orcid0000-0002-6989-1169
person.identifier.orcid0000-0002-6801-4578
person.identifier.orcid0000-0001-6218-4413
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridJ-2172-2014
person.identifier.ridG-5488-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id16305323200
person.identifier.scopus-author-id6507978333
person.identifier.scopus-author-id7203013518
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
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