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Effect of extraction conditions on flavonoids, tannins, antioxidant and antimicrobial activities of chestnut outer shells (Castanea sativa Miller)

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dc.contributor.authorAfonso, Maria João A.P.S.
dc.contributor.authorDias, Rolando
dc.contributor.authorGomes, Catarina
dc.contributor.authorPereira, Ermelinda
dc.contributor.authorPérez Guerra, Nelson
dc.contributor.authorRamalhosa, Elsa
dc.date.accessioned2023-03-14T15:33:57Z
dc.date.available2023-03-14T15:33:57Z
dc.date.issued2022
dc.description.abstractDuring the chestnut processing, are generated high amounts of by-products such as chestnut outer shells. They are a promising source of bioactive compounds. This study aimed to evaluate the presence of bioactive compounds in chestnut outer shells (Castanea sativa Mill.), to valorize this by-product. Material and methods. Several extraction procedures were used, specifically different solvents (water, ethanol:water (80:20) and acetone:water (80:20)), combined with two extraction methods (stirring (ST) and ultrasounds (US)) and extraction times. Flavonoids, hydrolizable and condensed tannins, antioxidant activity, antimicrobial activity were determined in the extracts. HPLC-PDA analysis was made to identify some phenolic compounds. Results. The acetone:water (80:20) mixture was the best solvent to extract flavonoids and condensed tannins. With this solvent, the highest total reducing capacity, DPPH radical scavenging activity, and reducing power were also obtained. On the contrary, the most suitable extraction solution for hydrolysable tannins was ethanol:water (80:20). Gallic, (+)-catechin, and tannic acids were detected by HPLC-PDA. Only the extracts obtained by the US exhibited antimicrobial activity. Conclusion. It was stated that chestnut outer shells have high amounts of bioactive compounds. In the extractions, the solvent plays a higher role than the method applied.pt_PT
dc.description.sponsorshipThe authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES (PIDDAC) to CIMO (UIDB/00690/2020 and UIDP/00690/2020), SusTEC (LA/P/0007/2020) and GO-ClimCast Project [PDR2020-1.0.1-FEADER-032060].pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAfonso, Maria João A.P.S.; Dias, Rolando; Gomes, Catarina; Pereira, Ermelinda; Pérez Guerra, Nelson; Ramalhosa, Elsa (2022). Effect of extraction conditions on flavonoids, tannins, antioxidant and antimicrobial activities of chestnut outer shells (Castanea sativa Miller). São Paulo: Editora Científica Digital. p. 46-67. ISBN 978-65-5360-176-5pt_PT
dc.identifier.doi10.37885/220709526pt_PT
dc.identifier.isbn978-65-5360-176-5
dc.identifier.urihttp://hdl.handle.net/10198/27707
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherEditora Científica Digitalpt_PT
dc.relationLA/P/0007/2020pt_PT
dc.relationMountain Research Center
dc.relationMountain Research Center
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectOuter shellpt_PT
dc.subjectChestnutpt_PT
dc.subjectBioactive compoundpt_PT
dc.subjectExtraction methodpt_PT
dc.subjectSolventpt_PT
dc.titleEffect of extraction conditions on flavonoids, tannins, antioxidant and antimicrobial activities of chestnut outer shells (Castanea sativa Miller)pt_PT
dc.typebook part
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardTitleMountain Research Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.citation.endPage67pt_PT
oaire.citation.startPage46pt_PT
oaire.citation.titleOpen Science Research Vpt_PT
oaire.citation.volume5pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameAfonso
person.familyNameDias
person.familyNameGomes
person.familyNamePereira
person.familyNameRamalhosa
person.givenNameMaria João A.P.S.
person.givenNameRolando
person.givenNameCatarina
person.givenNameErmelinda
person.givenNameElsa
person.identifier.ciencia-id721A-F98D-9A1A
person.identifier.ciencia-id8417-4D84-973B
person.identifier.ciencia-id2B12-75A1-B8FB
person.identifier.ciencia-id3916-218E-1629
person.identifier.ciencia-id1A1D-FC05-A05D
person.identifier.orcid0000-0002-2569-9896
person.identifier.orcid0000-0001-7369-382X
person.identifier.orcid0000-0003-3396-3615
person.identifier.orcid0000-0002-9431-5059
person.identifier.orcid0000-0003-2503-9705
person.identifier.ridM-8552-2013
person.identifier.scopus-author-id7102885104
person.identifier.scopus-author-id57190981477
person.identifier.scopus-author-id35222107200
person.identifier.scopus-author-id6602978189
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typebookPartpt_PT
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