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Chromatographic techniques to obtain the biomolecules profile of inedible wild mushrooms with antioxidant value

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dc.contributor.authorPereira, Eliana
dc.contributor.authorReis, Filipa S.
dc.contributor.authorBarros, Lillian
dc.contributor.authorMartins, Anabela
dc.contributor.authorFerreira, Isabel C.F.R.
dc.date.accessioned2012-08-17T10:12:25Z
dc.date.available2012-08-17T10:12:25Z
dc.date.issued2012
dc.description.abstractThe use of natural products isolated from mushrooms, included inedible species, against infection, cancer diseases and other oxidative-stress related diseases is one of the cornerstones of modern medicine [1]. In the present work, the antioxidant molecule profiles of inedible mushroom species were evaluated and compared with those of edible species. Mono and oligosaccharides {sugars), fatty acids and tocopherols were analysed by high performance liquid chromatography coupled to refraction index detection {HPLC/RI) , HPLC coupled to fluorescence and gas chromatography coupled to flame ionization detection {GC/FID), respectively. Ascorbic acid, carotenoids, phenolics and flavonoids were obtained by spectrophotometric techniques. The analysed molecules were further related to antioxidant properties, evaluated by free radical scavenging activity, reducing power and lipid peroxidation inhibition. The order of antioxidant abundance found in inedible wild mushrooms was: phenolics > flavonoids > ascorbic acid > tocopherols > carotenoids, similar to that of edible species. Furthermore the same energetic biomolecules were found including the disaccharide trehalose, the monosaccharide alcohol derivative mannitol and the fatty acids palmitic, oleic and linoleic acids. Fomitopsis pinicola revealed a very high phenolics concentrat ion {388 mg GAE/g extract) and powerful antioxidant properties, mainly reducing power { EC50 value 60 ug/ml similar to the standard Trolox®). It could find applications in the prevention of free radical-related diseases as a source of bioactive compounds.por
dc.identifier.citationPereira, Eliana; Reis, Filipa S.; Barros, Lillian; Martins, Anabela; Ferreira, Isabel C.F.R. (2012). Chromatographic techniques to obtain the biomolecules profile of inedible wild mushrooms with antioxidant value. In 7º Encontro Nacional de Cromatografia. Portopor
dc.identifier.urihttp://hdl.handle.net/10198/7351
dc.language.isoengpor
dc.peerreviewedyespor
dc.relationBPD/4609/2008
dc.relationPortuguese Wild Mushrooms: Chemical characterization and functional study of antiproliferative and proapoptotic properties in cancer cell lines
dc.titleChromatographic techniques to obtain the biomolecules profile of inedible wild mushrooms with antioxidant valuepor
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitlePortuguese Wild Mushrooms: Chemical characterization and functional study of antiproliferative and proapoptotic properties in cancer cell lines
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FAGR-ALI%2F110062%2F2009/PT
oaire.citation.conferencePlacePortopor
oaire.citation.title7º Encontro Nacional de Cromatografia, 9 a 11 de Janeiro de 2012por
oaire.fundingStream5876-PPCDTI
person.familyNamePereira
person.familyNameReis
person.familyNameBarros
person.familyNameMartins
person.familyNameFerreira
person.givenNameEliana
person.givenNameFilipa S.
person.givenNameLillian
person.givenNameAnabela
person.givenNameIsabel C.F.R.
person.identifier469085
person.identifier144781
person.identifier.ciencia-idBE17-462D-9A75
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person.identifier.orcid0000-0003-2854-6745
person.identifier.orcid0000-0002-9159-0530
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0001-6218-4413
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridQ-8017-2018
person.identifier.ridI-2093-2013
person.identifier.ridJ-3600-2013
person.identifier.ridG-5488-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id55938912300
person.identifier.scopus-author-id36982144400
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id7203013518
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typeconferenceObjectpor
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