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Haemorheological changes during recombinante human erythropoietin therapy in a rat model of renal failure induced by partial nephrectomy

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dc.contributor.authorCosta, Elísio
dc.contributor.authorReis, Flávio
dc.contributor.authorRocha-Pereira, Petronila
dc.contributor.authorBaptista, Sofia
dc.contributor.authorDias, André
dc.contributor.authorRocha, Susana
dc.contributor.authorCastro, Elisabeth
dc.contributor.authorMiranda, Vasco
dc.contributor.authorFaria, Maria Sameiro
dc.contributor.authorLoureiro, Alfredo
dc.contributor.authorLemos, Edite Teixeira de
dc.contributor.authorParada, Belmiro
dc.contributor.authorFigueiredo, Arnaldo
dc.contributor.authorQuintanilha, Alexandre
dc.contributor.authorTeixeira, Frederico
dc.contributor.authorBelo, Luís
dc.contributor.authorSantos-Silva, Alice
dc.date.accessioned2015-07-30T09:12:55Z
dc.date.available2015-07-30T09:12:55Z
dc.date.issued2008
dc.description.abstractThe aim of this work was to study the effect of recombinant human erythropoietin (rhEPO) the rapy on haemorheological parameters, by using a rat model of chronic renal failure (CRF) induced by partial (3/4) nephrectomy. The study used adult male Wistar rats and was performed in three groups: a control one (n=6) and in two groups with induced chronic renal failure (n=9), being one of them submitted to rhEPO the rapy (n=4). Blood samples from the control group were collected at the beginning and at the end of the experimental procedure and from CRF rats at 3, 5, 8, 12 and 15 weeks after surgical partial nephrectomy. Haemorheology and renal function were evaluated. Three weeks after the 3/4 nephrectomy, a statistically significant increase in serum urea and creatinine concentrations were found. This increase in renal function markers remained high along the 12 weeks of experimental procedure. Comparing with controls, rhEPO treated rat have showed a statistically significant progressive increase in haemoglobin (Hb), haematocrit (Ht), red blood cells (RBC) count, mean cell volume (MCV), mean cell Hb (MCH) and red cell distribution width (RDW), showing at 12 weeks an inverse change, though still presenting significant higher values; a decrease in platelet counts, during the first 9 weeks of rhEPO therapy. When comparing haemorheological data from non-treated CRF and controls, we found only a trend to increased MCV and MCH values and a decrease in reticulocyte count. Comparing the two groups of CRF rats, we found that rhEPO treated rats presented significantly higher values in RBC, Hb, Ht and RDW. In both groups of CRF rats, at five weeks, there was a decrease in their values, showing at the end a significantly lower value when compared to controls. No consistent alterations were found in white blood cells in CRF rats, with or without rhEPO therapy. Partial nephrectomy seems to be a suitable methodology to induce CRF in rats and to study erythropoiesis biology. The rhEPO therapy is associated with an increased erythropoietic stimulation and a decrease in platelet count.por
dc.identifier.citationRocha, Susana; Castro, Elisabeth; Miranda, Vasco; Faria, Maria Sameiro; Loureiro, Alfredo; Lemos, Edite Teixeira de; Parada, Belmiro; Figueiredo, Arnaldo; Quintanilha, Alexandre; Teixeira, Frederico; Belo; Luís, Santos-Silva, Alice (2008). Haemorheological changes during recombinante human erythropoietin therapy in a rat model of renal failure induced by partial nephrectomy. In Actas de Bioquímica. Lisboa. p. 125-129. ISBN 972-590-076-6por
dc.identifier.isbn972-590-076-6
dc.identifier.urihttp://hdl.handle.net/10198/12004
dc.language.isoporpor
dc.peerreviewedyespor
dc.subjectrhEPOpor
dc.subjectErythropoietinpor
dc.subjectRenal failurepor
dc.subjectRat modelpor
dc.subjectHaemorheologypor
dc.titleHaemorheological changes during recombinante human erythropoietin therapy in a rat model of renal failure induced by partial nephrectomypor
dc.typeconference object
dspace.entity.typePublication
rcaap.rightsopenAccesspor
rcaap.typeconferenceObjectpor

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