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Characterization of the biological residue Brassica oleracea L. to obtain a potencial natural ingredient

dc.contributor.authorPepinelli, Ana Luísa
dc.contributor.authorOliveira, Tatiane C.G.
dc.contributor.authorCaleja, Cristina
dc.contributor.authorDias, Maria Inês
dc.contributor.authorKostić, Marina
dc.contributor.authorSoković, Marina
dc.contributor.authorGonçalves, Odinei Hess
dc.contributor.authorOliveira, Beatriz
dc.contributor.authorPereira, Eliana
dc.contributor.authorBarros, Lillian
dc.date.accessioned2023-03-21T12:13:19Z
dc.date.available2023-03-21T12:13:19Z
dc.date.issued2022
dc.description.abstractThe food industry has been looking for natural matrices with potential application as natural ingredients. In this sense, the aim of this work was to characterize the Brassica oleracea var. Acephala biowastes, popularly known as kale, regarding its individual phenolic profile and biological action, in order to identify phenolic compounds with preservative and/or bioactive potential for incorporation in the food industry. The individual phenolic profile was obtained using spectrophotometric methods for an initial scan, and then HPLC-DAD/ESI-MSn was used for a detailed identification. The bioactive potential was evaluated through in vitro assays, analyzing the cytotoxicity in human tumor cell lines (AGS, CaCo-2, MCF-7, NCI-H460) and the hepatotoxicity in a non-tumor cell line (VERO), by the sulforhodamine B assay. The anti-inflammatory activity was studied in RAW 264.7 cells, the antimicrobial activity by the microdilution method, and the antioxidant activity by TBARS and CAA assays. The composition of phenolic compounds, namely the content of total phenols and total flavonoids, revealed a promising amount of these compounds, with concentrations of 5 mg GAE/g of total phenols and 2.4 mg EC/g of total flavonoids.pt_PT
dc.description.sponsorshipThe Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2021), for T.C.G. Oliveira. grant (2021.06046.BD) and M.I. Dias, E. Pereira and L. Barros contracts. Conducted under the project “BIOMA – Bioeconomy integrated solutions for the mobilization of the Agro-food market” (POCI-01-0247-FEDER-046112).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPepinelli, Ana Luísa; Oliveira, Tatiane C.G.; Caleja, Cristina; Dias, Maria Inês; Kostić, Marina; Soković, Marina; Gonçalves, Odinei Hess; Oliveira, M. Beatriz; Pereira, Eliana; Barros, Lillian (2022). Characterization of the biological residue Brassica oleracea L. to obtain a potencial natural ingredient. In Proceedings of 2nd International Congress on Bioactive Compounds - Galoá Proceedings. Campinaspt_PT
dc.identifier.urihttp://hdl.handle.net/10198/27903
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherICBCpt_PT
dc.relationLA/P/0007/2021pt_PT
dc.relationMountain Research Center
dc.relationMountain Research Center
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectPhenolic Compoundspt_PT
dc.subjectBioactivitypt_PT
dc.subjectNatural Ingredientspt_PT
dc.subjectResearch Subject Categories::TECHNOLOGY::Chemical engineering::Food technologypt_PT
dc.titleCharacterization of the biological residue Brassica oleracea L. to obtain a potencial natural ingredientpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardTitleMountain Research Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.citation.conferencePlaceCampinaspt_PT
oaire.citation.endPage2pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleProceedings of 2nd International Congress on Bioactive Compounds - Galoá Proceedingspt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyName Oliveira
person.familyNameCaleja
person.familyNameDias
person.familyNameGonçalves
person.familyNamePereira
person.familyNameBarros
person.givenNameTatiane C. G.
person.givenNameCristina
person.givenNameMaria Inês
person.givenNameOdinei Hess
person.givenNameEliana
person.givenNameLillian
person.identifier469085
person.identifier.ciencia-id8412-9342-DFCA
person.identifier.ciencia-idE218-8DD3-4959
person.identifier.ciencia-id2A13-4BE6-C7CF
person.identifier.ciencia-idBE17-462D-9A75
person.identifier.ciencia-id9616-35CB-D001
person.identifier.orcid0000-0002-2117-7943
person.identifier.orcid0000-0002-5769-8484
person.identifier.orcid0000-0001-8744-7814
person.identifier.orcid0000-0002-9528-8187
person.identifier.orcid0000-0003-2854-6745
person.identifier.orcid0000-0002-9050-5189
person.identifier.ridM-8242-2013
person.identifier.ridQ-8017-2018
person.identifier.ridJ-3600-2013
person.identifier.scopus-author-id36514202500
person.identifier.scopus-author-id54388787000
person.identifier.scopus-author-id9335958400
person.identifier.scopus-author-id55938912300
person.identifier.scopus-author-id35236343600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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