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Prevalence of shigatoxigenic Escherichia coli in bovine cattle: a survey in the North Region of Portugal

2019Conference object Open access
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dc.contributor.authorBallem, Andressa
dc.contributor.authorGonçalves, Soraia
dc.contributor.authorFerreira, A.I.
dc.contributor.authorFernandes, Conceição
dc.contributor.authorSaavedra, Maria José
dc.contributor.authorPinto, C.
dc.contributor.authorOliveira, H.
dc.contributor.authorAlmeida, Gonçalo
dc.contributor.authorAlmeida, Carina
dc.date.accessioned2020-03-31T14:55:09Z
dc.date.available2020-03-31T14:55:09Z
dc.date.issued2019
dc.description.abstractShiga toxin-producing Escherichia coli (STEC) strains are food-borne pathogens that can be transmitted to humans through many different routes, but mainly through consumption of contaminated foods. STEC strains are characterized by the production of Shiga toxins (Stx) that are encoded by two genes stx1 and/or stx2. Each toxin can be subdivided into subtypes and, currently, there are three known subtypes for stx1 (stx1a, stx1c, and stx1d) and seven for stx2 (stx2a, stx2b, stx2c, stx2d, stx2e, stx2f, and stx2g). Others virulence factors, such the production of intimin protein, encoded by eae gene, are associated with severe outcomes of STEC-associated diseases. The most common STEC serotype implicated worldwide is E. coli O157:H7, but many other STEC strains are associated with severe human diseases. Ruminants, especially cattle, are a major reservoir for O157 and non-O157 STEC. Information on STEC prevalence in Portuguese dairy cattle is limited, so we analysed for the presence of STEC 329 faecal specimens collected from the rectum of healthy dairy cattle. The samples were collected from adult lactating cows (n=194) and from heifers (n=135) with ages among 6 to 18 months, between March and June 2019, at milk farms (n=17) in the North region of Portugal. After enrichment, in modified TSB with novobiocin, samples were analysed by real time PCR to detect the presence of stx1, stx2 and eae in accordance to ISO/TS 13136:2012(E). In addition, conventional PCR for the detection of sxt1 and stx2 gene subtypes was performed according to the guidelines of the VTEC European Union Referencept_PT
dc.description.sponsorshipThis study was supported by project PhageSTEC (POCI-01-0145-FEDER-029628) funded by FEDER through COMPETE2020 (Programa Operacional Competitividade e Internacionalização) and by National Funds thought FCT (Fundação para a Ciência e a Tecnologia).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBallem, A.; Gonçalves, S.; Ferreira, A.I.; Fernandes, C.; Saavedra, M.J.; Pinto, C.; Oliveira, H.; Almeida, Gonçalo; Almeida, Carina (2019). Prevalence of shigatoxigenic Escherichia coli in bovine cattle: a survey in the North Region of Portugal. In 1º Simpósio INIAV para a Segurança Alimentar. Vila do Condept_PT
dc.identifier.urihttp://hdl.handle.net/10198/21287
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherINIAVpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectFood safetypt_PT
dc.subjectShiga toxinpt_PT
dc.subjectPathogenspt_PT
dc.subjectE. colipt_PT
dc.subjectCattlept_PT
dc.titlePrevalence of shigatoxigenic Escherichia coli in bovine cattle: a survey in the North Region of Portugalpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceVila do Condept_PT
oaire.citation.endPage159pt_PT
oaire.citation.startPage158pt_PT
oaire.citation.title1º Simpósio INIAV para a Segurança Alimentar (SAlimentar 2019)pt_PT
person.familyNameFernandes
person.givenNameConceição
person.identifier15483
person.identifier.ciencia-id6514-3D41-4534
person.identifier.orcid0000-0003-2873-501X
person.identifier.scopus-author-id23018336600
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationd9c26c54-5e10-4ccb-b7f7-528af9eb9950
relation.isAuthorOfPublication.latestForDiscoveryd9c26c54-5e10-4ccb-b7f7-528af9eb9950

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