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Clitocybe alexandri extract induces cell cycle arrest and apoptosis in a lung cancer cell line: identification of phenolic acids with cytotoxic potential

2012Journal article Open access
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dc.contributor.authorVaz, Josiana A.
dc.contributor.authorAlmeida, Gabriela M.
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorMartins, Anabela
dc.contributor.authorVasconcelos, M. Helena
dc.date.accessioned2012-08-21T10:51:55Z
dc.date.available2012-08-21T10:51:55Z
dc.date.issued2012
dc.description.abstractMushrooms are a possible rich source of biologically active compounds with potential for drug discovery. The aim of this work was to gain further insight into the citotoxicity mechanism of action of Clitocybe alexandri ethanolic extract against a lung cancer cell line (NCI-H460 cells). The effects on cell cycle profile and levels of apoptosis were evaluated by flow cytometry, and the effect on the expression levels of proteins related to cellular apoptosis was also investigated by Western blot. The extract was characterized regarding its phenolic composition by HPLC-DAD, and the identified compounds were studied regarding their growth inhibitory activity, by sulforhodamine B (SRB) assay. The effect of individual or combined compounds on viable cell number was also evaluated using the Trypan blue exclusion assay. It was observed that the Clitocybe alexandri extract induced an S-phase cell cycle arrest and increased the percentage of apoptotic cells. In addition, treatment with the GI50 concentration (concentration that was able to cause 50% of cell growth inhibition; 24.8 µg/ml) for 48h caused an increase in the levels of wt p53, cleaved caspase-3 and cleaved poly (ADP-ribose) polymerase (PARP). The main components identified in this extract were protocatechuic, p-hydroxybenzoic and cinnamic acids. Cinnamic acid was found to be the most potent compound regarding cell growth inhibition. Nevertheless, it was verified that the concomitant use of the individual compounds provided the strongest decrease in viable cell number. Overall, we found evidence for alterations in cell cycle and apoptosis, involving p53 and caspase-3. Furthermore, our data suggests that the phenolic acids identified in the extract are at least partially responsible for the cytotoxicity induced by this mushroom extract.por
dc.identifier.citationVaz, Josiana A.; Almeida, Gabriela M.; Ferreira, Isabel C.F.R.; Martins, Anabela; Vasconcelos, M. Helena (2012). Clitocybe alexandri extract induces cell cycle arrest and apoptosis in a lung cancer cell line: identification of phenolic acids with cytotoxic potential. Food Chemistry. ISSN 0308-8146. 132:1, p. 482-486por
dc.identifier.doi10.1016/j.foodchem.2011.11.031
dc.identifier.issn0308-8146
dc.identifier.urihttp://hdl.handle.net/10198/7374
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationBD/43653/2008
dc.subjectClitocybe alexandripor
dc.subjectPhenolic acidspor
dc.subjectCitotoxicitypor
dc.subjectCell cyclepor
dc.subjectApoptosispor
dc.titleClitocybe alexandri extract induces cell cycle arrest and apoptosis in a lung cancer cell line: identification of phenolic acids with cytotoxic potentialpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/c
oaire.citation.endPage486por
oaire.citation.startPage482por
oaire.citation.titleFood Chemistrypor
oaire.citation.volume132por
oaire.fundingStream5876-PPCDTI
person.familyNameVaz
person.familyNameFerreira
person.familyNameMartins
person.givenNameJosiana A.
person.givenNameIsabel C.F.R.
person.givenNameAnabela
person.identifier144781
person.identifier.ciencia-id171B-88BA-64F5
person.identifier.ciencia-id9418-CF95-9919
person.identifier.ciencia-id7B18-A810-6B93
person.identifier.orcid0000-0002-6989-1169
person.identifier.orcid0000-0003-4910-4882
person.identifier.orcid0000-0001-6218-4413
person.identifier.ridE-8500-2013
person.identifier.ridG-5488-2013
person.identifier.scopus-author-id16305323200
person.identifier.scopus-author-id36868826600
person.identifier.scopus-author-id7203013518
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication1c1a2cb9-5a36-47ba-bca0-ffcbef28df18
relation.isAuthorOfPublicationbd0d1537-2e03-41fb-b27a-140af9c35db8
relation.isAuthorOfPublication383370dd-10e5-40e6-9a15-f2201fe95581
relation.isAuthorOfPublication.latestForDiscovery383370dd-10e5-40e6-9a15-f2201fe95581
relation.isProjectOfPublication194d35a5-12df-4f40-97d4-7bafd50a2f34
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