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Effect of stirring and ultrasound-assisted extraction conditions in flavonoids, tannins, antioxidant, antimicrobial activities of the outer skin of chestnuts (Castanea sativa Miller)

2021Conference object Open access
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dc.contributor.authorAfonso, Maria João A.P.S.
dc.contributor.authorDias, Rolando
dc.contributor.authorGomes, Catarina
dc.contributor.authorPereira, Ermelinda
dc.contributor.authorGuerra, Nelson P.
dc.contributor.authorRamalhosa, Elsa
dc.date.accessioned2023-03-24T12:41:37Z
dc.date.available2023-03-24T12:41:37Z
dc.date.issued2021
dc.description.abstractThis study aimed to evaluate the presence of bioactive antioxidant molecules in chestnut outer shells. For this proposal, several extraction procedures were used, specifically different solvents (water, ethanol:water (80:20) and acetone:water (80:20)) combined with two extraction methods (stirring and ultrasounds (US)) and times of extraction. Several chemical assays were applied to evaluate the flavonoids, hydrolysable and condensed tannins contents, and the antioxidant activity by total reducing capacity, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, and reducing power. Some individual compounds were identified by HPLC-UV detection. Furthermore, the antimicrobial activity of the obtained extracts was screened using different microorganisms, namely Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Enterococcus faecalis) and Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Enterobacter aerogenes, Proteus mirabilis, Klebsiella pneumoniae), as well as fungi (Candida albicans). The mixture acetone:water (80:20) was the best solvent to extract condensed tannins and flavonoids. It was with this mixture that the best total reducing capacity, (DPPH) radical scavenging activity and reducing power were determined. The highest extraction yield was also obtained with this mixture. Only for the hydrolysable tannins, the most suitable extraction solution was ethanol:water (80:20). The presence of gallic and tannic acids was detected by HPLC-UV. Concerning the extraction methods, only the extracts obtained by the US exhibited antimicrobial activity. The ethanolic extract showed antimicrobial activity against the Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Enterococcus faecalis) and the extracts with acetone:water (80:20) and water showed antimicrobial activity against Proteus mirabilis. The results of this study demonstrated that the chestnut outer shell is a promising source of bioactive compounds.pt_PT
dc.description.sponsorshipFCT, Portugal [CIMO: UID/AGR/00690/2019]; Portugal 2020 [Go_ClimCast Project: PDR2020- 1.0.1-FEADER-032060]pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMaria João A.P.S.; Dias, Rolando; Gomes, Catarina; Pereira, Ermelinda; Guerra, Nelson; Ramalhosa, Elsa (2021). Effect of stirring and ultrasound-assisted extraction conditions in flavonoids, tannins, antioxidant, antimicrobial activities of the outer skin of chestnuts (Castanea sativa Miller). In Margarida Vieira; Paola Pittia; Cristina L.M. Silva; Florence Dubois-Brissonnet; Rui Costa; Foteini Chrysanthopoulou (Eds.) 6th International ISEKI-Food Conference: Sustainable Development Goals in Food Systems: Challenges and Opportunities for the Future: Book of Abstracts. Online
dc.identifier.doi10.34623/9hhy-1y83
dc.identifier.isbn978-989-9023-52-9
dc.identifier.urihttp://hdl.handle.net/10198/28040
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherISEKI - Food Associationpt_PT
dc.relationMountain Research Centre
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBioactive compoundspt_PT
dc.subjectEthanolpt_PT
dc.subjectAcetonept_PT
dc.subjectWaterpt_PT
dc.subjectChestnut shellpt_PT
dc.titleEffect of stirring and ultrasound-assisted extraction conditions in flavonoids, tannins, antioxidant, antimicrobial activities of the outer skin of chestnuts (Castanea sativa Miller)pt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleMountain Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FAGR%2F00690%2F2019/PT
oaire.citation.conferencePlaceOnlinept_PT
oaire.citation.title6th International ISEKI - Food Conference: Sustainable Development Goals in Food Systems: Challenges and opportunities for the futurept_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameAfonso
person.familyNameDias
person.familyNameGomes
person.familyNamePereira
person.familyNameRamalhosa
person.givenNameMaria João A.P.S.
person.givenNameRolando
person.givenNameCatarina
person.givenNameErmelinda
person.givenNameElsa
person.identifier.ciencia-id721A-F98D-9A1A
person.identifier.ciencia-id8417-4D84-973B
person.identifier.ciencia-id2B12-75A1-B8FB
person.identifier.ciencia-id3916-218E-1629
person.identifier.ciencia-id1A1D-FC05-A05D
person.identifier.orcid0000-0002-2569-9896
person.identifier.orcid0000-0001-7369-382X
person.identifier.orcid0000-0003-3396-3615
person.identifier.orcid0000-0002-9431-5059
person.identifier.orcid0000-0003-2503-9705
person.identifier.ridM-8552-2013
person.identifier.scopus-author-id7102885104
person.identifier.scopus-author-id57190981477
person.identifier.scopus-author-id35222107200
person.identifier.scopus-author-id6602978189
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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