Publication
Towards honey authentication: Differentiation of Apis mellifera subspecies in European honeys based on mitochondrial DNA markers
| dc.contributor.author | Soares, Sónia | |
| dc.contributor.author | Grazina, Liliana | |
| dc.contributor.author | Mafra, Isabel | |
| dc.contributor.author | Costa, Joana | |
| dc.contributor.author | Pinto, M. Alice | |
| dc.contributor.author | Oliveira, Beatriz | |
| dc.contributor.author | Amaral, Joana S. | |
| dc.date.accessioned | 2018-02-19T10:00:00Z | |
| dc.date.accessioned | 2020-01-06T14:10:46Z | |
| dc.date.available | 2018-01-19T10:00:00Z | |
| dc.date.available | 2020-01-06T14:10:46Z | |
| dc.date.issued | 2019 | |
| dc.description.abstract | Honey is the natural sweet substance produced by Apis mellifera honeybees in Europe. Depending on the country/region, the A. mellifera subspecies native to Europe belong to three different lineages: A (A. m. iberiensis), M (A. m. iberiensis and A. m. mellifera) and C (A. m. ligustica and A. m. carnica). In this work, two DNAbased approaches were developed with the aim of entomological authentication of European honeys. A cytb specific PCR assay was proposed to identify A-lineage honeybees, while a second method based on real-time PCR coupled to high resolution melting analysis targeting the COI gene was developed to differentiate C- and Mlineages honeybees. The proposed methodologies were validated successfully with honeys of known origin and applied to the entomological authentication of 20 commercial samples from different European countries. The results highlight the predominance of honeys from C-lineage honeybees in Europe, except in Iberian Peninsula countries (honey from A-lineage honeybees). | |
| dc.description.sponsorship | The authors are grateful to Dora Henriques for assembling the mitogenomes and to Pilar de la Rua and António Pajuelo for supplying authentic honey samples. This work was supported by FCT (Fundação para a Ciência e Tecnologia) through project UID/QUI/50006/2013 – POCI/01/0145/FEDER/007265 with financial support from FCT/MEC through national funds and co-financed by FEDER, under the Partnership Agreement PT2020 and by the projects NORTE-01-0145- FEDER-000011 and BeeHappy – POCI-01-0145-FEDER-029871 (financed by FEDER through the COMPETE 2020 – Operational Programme for Competitiveness and Internationalisation (POCI) and FCT). S. Soares, L. Grazina and J. Costa are grateful to FCT grants (SFRH/BD/75091/2010, SFRH/BD/132462/2017 and SFRH/BPD/102404/2014, respectively) financed by POPH-QREN (subsidised by FSE and MCTES). | |
| dc.description.version | info:eu-repo/semantics/publishedVersion | en_EN |
| dc.identifier.citation | Soares, Sónia; Grazina, Liliana; Mafra, Isabel; Costa, Joana; Pinto, M. Alice; Oliveira, M. Beatriz P.P.; Amaral, Joana S. (2019). Towards honey authentication: Differentiation of Apis mellifera subspecies in European honeys based on mitochondrial DNA markers. Food Chemistry. ISSN 0308-8146. 283, p. 294-301 | en_EN |
| dc.identifier.doi | 10.1016/j.foodchem.2018.12.119 | en_EN |
| dc.identifier.issn | 0308-8146 | |
| dc.identifier.uri | http://hdl.handle.net/10198/20290 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | en_EN |
| dc.relation | 102404/2014 | |
| dc.subject | Apis mellifera subspecies | en_EN |
| dc.subject | Authenticity | en_EN |
| dc.subject | Honey | en_EN |
| dc.subject | Honeybee DNA | en_EN |
| dc.subject | HRM analysis | en_EN |
| dc.title | Towards honey authentication: Differentiation of Apis mellifera subspecies in European honeys based on mitochondrial DNA markers | en_EN |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| person.familyName | Pinto | |
| person.familyName | Amaral | |
| person.givenName | M. Alice | |
| person.givenName | Joana S. | |
| person.identifier.ciencia-id | F814-A1D0-8318 | |
| person.identifier.ciencia-id | 5319-7DE8-BEDA | |
| person.identifier.orcid | 0000-0001-9663-8399 | |
| person.identifier.orcid | 0000-0002-3648-7303 | |
| person.identifier.scopus-author-id | 8085507800 | |
| rcaap.rights | openAccess | en_EN |
| rcaap.type | article | en_EN |
| relation.isAuthorOfPublication | 0667fe04-7078-483d-9198-56d167b19bc5 | |
| relation.isAuthorOfPublication | 42be2cf4-adc4-4e7f-ac60-7aab515b38cd | |
| relation.isAuthorOfPublication.latestForDiscovery | 0667fe04-7078-483d-9198-56d167b19bc5 |