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Cell Wall-Mediated Antifungal Activity of the Aqueous Extract of Hedera helix L. Leaves Against Diplodia corticola

2024Journal article Open access
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dc.contributor.authorCrisóstomo, Christina
dc.contributor.authorSimões, Luara
dc.contributor.authorBarros, Lillian
dc.contributor.authorFinimundy, Tiane C.
dc.contributor.authorCunha, Ana
dc.contributor.authorOliveira, Rui P.
dc.date.accessioned2025-01-14T14:37:04Z
dc.date.available2025-01-14T14:37:04Z
dc.date.issued2024
dc.description.abstractCork oak forests have been declining due to fungal pathogens such as Diplodia corticola. However, the preventive fungicides against this fungus have restricted use due to the deleterious effects on human health and the environment, prompting the need for sustainable alternatives. Here, we describe the antifungal activity of an aqueous extract of Hedera helix L. leaves (HAE) against D. corticola and the possible mechanism of action. Results/Methods: The chemical analysis revealed compounds like the saponin hederacoside C, quinic acid, 5-O-caffeoylquinic acid, rutin, and glycoside derivatives of quercetin and kaempferol, all of which have been previously reported to possess antimicrobial activity. Remarkable in vitro antifungal activity was observed, reducing radial mycelial growth by 70% after 3 days of inoculation. Saccharomyces cerevisiae mutants, bck1 and mkk1/mkk2, affected the cell wall integrity signaling pathway were more resistant to HAE than the wild-type strain, suggesting that the extract targets kinases of the signaling pathway, which triggers toxicity. The viability under osmotic stress with 0.75 M NaCl was lower in the presence of HAE, suggesting the deficiency of osmotic protection by the cell wall. Conclusions: These results suggest that ivy extracts can be a source of new natural antifungal agents targeting the cell wall, opening the possibility of preventing fungal infections in cork oaks and improving the cork production sector using safer and more sustainable approaches.pt_PT
dc.description.sponsorshipThis work was supported by the “Contrato-Programa” UIDB/04050/2020 funded by national funds through the FCT I.P (https://doi.org/10.54499/UIDB/04050/2020) and by AgrifoodXXI (NORTE-01-0145-FEDER-000041). This work was also supported by national funds through FCT/MCTES (PIDDAC): CIMO, UIDB/00690/2020 (DOI: 10.54499/UIDB/00690/2020), UIDP/00690/2020 (DOI: 10.54499/UIDP/00690/2020), and SusTEC, LA/P/0007/2020 (DOI: 10.54499/LA/P/0007/2020). National funding was provided by FCT to L. Barros through the scientific employment program-contract (CEEC-INST, DOI: 10.54499/CEECINST/00107/2021/CP2793/CT0002).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCrisóstomo, Christina; Simões, Luara; Barros, Lillian; Finimundy, Tiane C.; Cunha, Ana; Oliveira, Rui (2024). Cell Wall-Mediated Antifungal Activity of the Aqueous Extract of Hedera helix L. Leaves Against Diplodia corticola. Antibiotics. ISSN 2079-6382. 13:12, p. 1-15pt_PT
dc.identifier.doi10.3390/antibiotics13121116pt_PT
dc.identifier.issn2079-6382
dc.identifier.urihttp://hdl.handle.net/10198/30977
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationCENTRE OF MOLECULAR AND ENVIRONMENTAL BIOLOGY
dc.relationMountain Research Center
dc.relationMountain Research Center
dc.relationAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHedera helix water extractpt_PT
dc.subjectAntifungal mechanism of actionpt_PT
dc.subjectAnti-phytopathogen activitypt_PT
dc.subjectDiplodia corticolapt_PT
dc.subjectSaccharomyces cerevisiae fungal modelpt_PT
dc.titleCell Wall-Mediated Antifungal Activity of the Aqueous Extract of Hedera helix L. Leaves Against Diplodia corticolapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCENTRE OF MOLECULAR AND ENVIRONMENTAL BIOLOGY
oaire.awardTitleMountain Research Center
oaire.awardTitleMountain Research Center
oaire.awardTitleAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04050%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0007%2F2020/PT
oaire.citation.endPage15pt_PT
oaire.citation.issue12pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleAntibioticspt_PT
oaire.citation.volume13pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameBarros
person.familyNameFinimundy
person.givenNameLillian
person.givenNameTiane C.
person.identifier469085
person.identifier.ciencia-id9616-35CB-D001
person.identifier.ciencia-id2F18-67B1-776A
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0003-3516-0795
person.identifier.ridJ-3600-2013
person.identifier.scopus-author-id35236343600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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