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Advisor(s)
Abstract(s)
Imaging analysis techniques have been extensively used to obtain
crucial information on blood phenomena in the microcirculation. In the present
study, it is intended to mimic the effects of malaria on the red blood cells
(RBCs), by changing their properties using a different concentration of glutaraldehyde
solution. The effects of the disease in stiffing RBCs were evaluated
using polydimethylsiloxane microchannels that comprise contractions with
10 μm width and measuring the cells deformability and the flow velocity in
healthy and modified conditions. The obtained results show a decrease in the
RBCs deformability and in the flow velocity with the presence of glutaraldehyde,
when compared to the behavior of healthy RBCs samples. Therefore, it
can be concluded that, using image analysis (ImageJ & PIVLab), it is possible to
measure the deformability of the RBCs and the flow velocity and, consequently,
obtaining a correlation between the difference of velocities/deformabilities in the
microchannels. In the future, this correlation can be used to relate the RBCs
behavior with the various stages of malaria. This study can be a starting point for
establishing the development of new malaria diagnostic systems towards
point-of-care lab-on-a-chip devices.
Description
Keywords
Microfluidic devices Living cells Deformation Fabrication
Citation
Vilas Boas, Liliana; Lima, Rui; Minas, Graça; Fernandes, Carla S.; Catarino, Susana O. (2018). Imaging of healthy and malaria-mimicked red blood cells in polydimethylsiloxane microchannels for determination of cells deformability and flow velocity. In 6th ECCOMAS Thematic Conference on Computational Vision and Medical Image Processing (VipIMAGE). Porto. p. 915-922. ISBN 978-3-319-68194-8