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Development of double emulsions from Sambucus nigra L. berry extract as ph stable colorant formulations

dc.contributor.authorTeixeira, Liandra Gracher
dc.contributor.authorFernandes, Isabel P.
dc.contributor.authorRezende, Stephany
dc.contributor.authorReis, Filipa S.
dc.contributor.authorBarros, Lillian
dc.contributor.authorLeimann, Fernanda Vitória
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorBarreiro, M.F.
dc.date.accessioned2021-10-29T10:22:14Z
dc.date.available2021-10-29T10:22:14Z
dc.date.issued2019
dc.description.abstractAnthocyanins are flavonoids, one of the largest groups of water-soluble vegetable pigments, having also sweetening and antioxidant properties. They are characterized by different colors, such as red, purple, blue and violet. However, their color stability is compromised by variations in pH, temperature, moisture, and luminosity conditions. 1 The berries of Sambucus nigra L. have high contents of anthocyanins (cyanidin-3-O-sambubioside and cyanidin-3-Osambubioside-5-O-glucoside), being a promising option as food coloring agents. 2 Therefore, this work aims to stabilize the natural hydrophilic colorant obtained from S. nigra through the preparation of double emulsions (water/oil/water, W/O/W). The S. nigra berries were extracted by crushing the fruits to obtain the juice, followed by spray drying to obtain the dry extract. For the preparation of the double emulsions, the procedure was the one described by Paula D. A. et al. with some modifications. 3 Briefly, for the first emulsion, the water phase comprised 55 mg/L of the extract and 5% Polyglycerol polyricinoleate (PGPR), homogenized under stirring (20000 rpm, 5 min). This phase was dispersed into corn oil at a W/O ratio of 0.4. (Figure 1 – (A)). Thereafter, the second emulsion was prepared by dispersing the previous emulsion in a water phase comprising Tween 80 and gum Arabic, tested at different concentrations. The final W/O/W emulsion was prepared at different primary emulsion/water ratios and stirred at 6000 rpm during 2 min using an Ultra-Turrax system. The produced emulsions were analyzed by optical microscopy (OM) using a Nikon eclipse 50i microscope to assess size and morphology. This analysis showed the presence of spherical and individualized droplets with an estimated particle size between 13 and 16 μm. (Figure 1 – (B)). The role of these emulsions as pH stable colorants were tested using different buffers (pH 4-7), as shown in (Figure 1-(C)), which also presented the comparison with solutions prepared from the original S. nigra extract. The obtained results pointed out for an improved stability and color homogeneity among samples in the tested pH range.pt_PT
dc.description.sponsorshipAssociate Laboratory LSRE-LCM (UID/EQU/50020/2019) funded by national funds through FCT/MCTES (PIDDAC) and FCT, and CIMO (UID/AGR/00690/2019) trough FEDER under Program PT2020. To project Valor Natural (Norte-01-0247-FEDER024479) trough FEDER under Program PT2020. To the national funding by FCT, P.I., through the institutional scientific employment program-contract for L. Barros and I.P. Fernandes contract.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationTeixeira, Liandra Gracher; Fernandes, Isabel Patricia Martins; Rezende, S.C.; Reis, Filipa S.; Barros, Lillian; Leimann, Fernanda Vitória; Ferreira, Isabel C.F.R.; Barreiro, M.F. (2019). Development of double emulsions from Sambucus nigra L. berry extract as ph stable colorant formulations. In Livro de resumos do XXVI Encontro Nacional da Sociedade Portuguesa de Química. ISBN 978-989-8124-27-2
dc.identifier.isbn978-989-8124-27-2
dc.identifier.urihttp://hdl.handle.net/10198/24133
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationLaboratory of Separation and Reaction Engineering
dc.relationMountain Research Centre
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectSambucus nigra L.pt_PT
dc.subjectpHpt_PT
dc.titleDevelopment of double emulsions from Sambucus nigra L. berry extract as ph stable colorant formulationspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleLaboratory of Separation and Reaction Engineering
oaire.awardTitleMountain Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FEQU%2F50020%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FAGR%2F00690%2F2019/PT
oaire.citation.conferencePlacePorto, Portugalpt_PT
oaire.citation.titleXXVI Encontro Nacional da Sociedade Portuguesa de Químicapt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameTeixeira
person.familyNameFernandes
person.familyNameRezende
person.familyNameReis
person.familyNameBarros
person.familyNameLeimann
person.familyNameFerreira
person.familyNameBarreiro
person.givenNameLiandra Gracher
person.givenNameIsabel P.
person.givenNameStephany
person.givenNameFilipa S.
person.givenNameLillian
person.givenNameFernanda Vitória
person.givenNameIsabel C.F.R.
person.givenNameFilomena
person.identifier469085
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person.identifier.orcid0000-0003-1385-2037
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person.identifier.orcid0000-0002-9159-0530
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0001-6230-9597
person.identifier.orcid0000-0003-4910-4882
person.identifier.orcid0000-0002-6844-333X
person.identifier.ridL-1552-2016
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person.identifier.scopus-author-id36868826600
person.identifier.scopus-author-id57188719717
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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