Repository logo
 
Publication

Ganoderma lucidum methanolic extract: chemical characterization in phenolic compounds and study of growth inhibitory activity in human tumour cell lines

2012Conference object Open access
Simple item page
dc.contributor.authorHeleno, Sandrina A.
dc.contributor.authorTavares, Catarina
dc.contributor.authorVaz, Josiana A.
dc.contributor.authorAlmeida, Gabriela M.
dc.contributor.authorMartins, Anabela
dc.contributor.authorQueiroz, Maria João R.P.
dc.contributor.authorVasconcelos, M. Helena
dc.contributor.authorFerreira, Isabel C.F.R.
dc.date.accessioned2013-05-24T13:36:46Z
dc.date.available2013-05-24T13:36:46Z
dc.date.issued2012
dc.description.abstractGanoderma lucidum is one of the most extensively studied mushroom species due to its medicinal properties. It has been used as functional food and as chemopreventer in some countries for thousands of years, and became a popular dietary supplement ingredient in Western countries [1]. Some of its pharmacological properties have been related to antitumour properties, attributed to a wide variety of bioactive components such as polysaccharides, triterpenes, sterols, lectins and some proteins [2,3]. Nevertheless, the bioactive properties of its phenolic compounds have not been studied. The aim of this work was to study the potential antitumor activity of the methanolic extract of this mushroom. This extract of Ganoderma lucidum, collected in Northeast Portugal, was characterized in phenolic compounds, by high performance liquid chromatography coupled to photodiode array detection and mass spectrometry (HPLC-DAD-MS). The extract was further submitted to evaluation of growth inhibitory activity in four human tumour cell lines (MCF-7, NCI-H460, HCT15 and AGS), by the sulforhodamine B assay. The extract presented a moderate growth inhibitory activity in all the cell lines tested (values of GI50 between 93.3±18.1 and 112.6±11.7μg/mL).The following compounds were identified in the extract: p-hydroxybenzoic acid (0.58 ± 0.04 mg/100 g dw), p-coumaric acid (0.38 ± 0.03 mg/100 g dw) and cinnamic acid (0.28 ± 0.03 mg/100 g dw). Future work will elucidate the mechanism of action of the studied extract leading to the observed cell growth inhibition.por
dc.description.sponsorshipFCT and FEDER COMPETE/QREN/EU through project PTDC/AGR-ALI/110062/2009 and through the research centres (PEst-C/QUI/UI0686/2011 and PEst-OE/AGR/UI0690/2011). S.A. Heleno also thanks to FCT for her PhD grant (SFRH/BD/70304/2010).por
dc.identifier.citationHeleno, Sandrina A.; Tavares, Catarina; Vaz, Josiana A.; Almeida, Gabriela M.; Martins, Anabela; Queiroz, Maria João R.P.; Vasconcelos, M. Helena (2012). Ganoderma lucidum methanolic extract: chemical characterization in phenolic compounds and study of growth inhibitory activity in human tumour cell lines. In XXIInd International Symposium on Medicinal Chemistry. Berlinpor
dc.identifier.urihttp://hdl.handle.net/10198/8421
dc.language.isoengpor
dc.peerreviewedyespor
dc.relationPortuguese Wild Mushrooms: Chemical characterization and functional study of antiproliferative and proapoptotic properties in cancer cell lines
dc.relationStrategic Project - UI 686 - 2011-2012
dc.relationStrategic Project - UI 690 - 2011-2012
dc.relationIMPORTANT HUMAN METABOLITES OF PHENOLIC ACIDS FROM DIET WITH WILD EDIBLE MUSHROOMS: CHEMICAL SYNTHESIS AND STUDIES OF THEIR ANTIOXIDANT AND ANTITUMOR PROPERTIES
dc.titleGanoderma lucidum methanolic extract: chemical characterization in phenolic compounds and study of growth inhibitory activity in human tumour cell linespor
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitlePortuguese Wild Mushrooms: Chemical characterization and functional study of antiproliferative and proapoptotic properties in cancer cell lines
oaire.awardTitleStrategic Project - UI 686 - 2011-2012
oaire.awardTitleStrategic Project - UI 690 - 2011-2012
oaire.awardTitleIMPORTANT HUMAN METABOLITES OF PHENOLIC ACIDS FROM DIET WITH WILD EDIBLE MUSHROOMS: CHEMICAL SYNTHESIS AND STUDIES OF THEIR ANTIOXIDANT AND ANTITUMOR PROPERTIES
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FAGR-ALI%2F110062%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FQUI%2FUI0686%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FAGR%2FUI0690%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F70304%2F2010/PT
oaire.citation.conferencePlaceBerlim, Alemanhapor
oaire.citation.titleXXIInd International Symposium on Medicinal Chemistry, 2-6th September 2012por
oaire.fundingStream5876-PPCDTI
oaire.fundingStream6820 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameHeleno
person.familyNameVaz
person.familyNameMartins
person.familyNameFerreira
person.givenNameSandrina A.
person.givenNameJosiana A.
person.givenNameAnabela
person.givenNameIsabel C.F.R.
person.identifier144781
person.identifier.ciencia-id8B18-3095-6FC9
person.identifier.ciencia-id171B-88BA-64F5
person.identifier.ciencia-id7B18-A810-6B93
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0001-7224-1098
person.identifier.orcid0000-0002-6989-1169
person.identifier.orcid0000-0001-6218-4413
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridL-5951-2014
person.identifier.ridG-5488-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id30067731800
person.identifier.scopus-author-id16305323200
person.identifier.scopus-author-id7203013518
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typeconferenceObjectpor
relation.isAuthorOfPublicatione89b5bf5-d315-49b6-871e-fa8eb6030676
relation.isAuthorOfPublication1c1a2cb9-5a36-47ba-bca0-ffcbef28df18
relation.isAuthorOfPublication383370dd-10e5-40e6-9a15-f2201fe95581
relation.isAuthorOfPublicationbd0d1537-2e03-41fb-b27a-140af9c35db8
relation.isAuthorOfPublication.latestForDiscovery383370dd-10e5-40e6-9a15-f2201fe95581
relation.isProjectOfPublication59db4b60-8cc2-4f07-a25a-8cfe78f2778f
relation.isProjectOfPublicationec5d1315-a47f-4629-907c-e4737edfafac
relation.isProjectOfPublicationc59f09d4-9931-4f2e-babb-ef8bed998be7
relation.isProjectOfPublication81c2dd9c-140a-47d1-b37c-514cb80df3c9
relation.isProjectOfPublication.latestForDiscovery81c2dd9c-140a-47d1-b37c-514cb80df3c9

Files

Original bundle
Now showing 1 - 2 of 2
No Thumbnail Available
Name:
Poster Int. 75.pdf
Size:
758.43 KB
Format:
Adobe Portable Document Format
Download
No Thumbnail Available
Name:
Resumo_Berlim_Sandrina.pdf
Size:
90.55 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CIMO - Resumos em Proceedings Não Indexados à WoS/Scopus