Advisor(s)
Abstract(s)
Evidence for the participation of reactive oxygen species (ROS) and antioxidant systems in ectomycorrhizal(ECM) establishment is lacking. In this paper, we evaluated
ROS production and the activities of superoxide dismutase
(SOD) and catalase (CAT) during the early contact of the
ECM fungus Pisolithus tinctorius with the roots of Castanea
sativa (chestnut tree). Roots were placed in contact with P.
tinctorius mycelia, and ROS production was evaluated by
determining the levels of H2O2 and O2
I− during the early
stages of fungal contact. Three peaks of H2O2 production
were detected, the first two coinciding with O2
I− bursts. The
first H2O2 production peak coincided with an increase in
SOD activity, whereas CAT activity seemed to be implicated
in H2O2 scavenging. P. tinctorius growth was evaluated in
the presence of P. tinctorius-elicited C. sativa crude extracts
prepared during the early stages of fungal contact. Differential
hyphal growth that matched the H2O2 production profile
with a delay was detected. The result suggests that during theearly stages of ECM establishment, H2O2 results from an
inhibition of ROS-scavenging enzymes and plays a role in
signalling during symbiotic establishment.
Description
Keywords
Castanea sativa Pisolithus tinctorius Ectomycorrhiza (ECM) Reactive oxygen species Catalase (CAT) Superoxide dismutase (SOD)
Citation
Baptista, Paula; Martins, Anabela; Pais, Maria Salomé; Tavares, Rui; Lino-Neto, Teresa (2007). Involvement of reactive oxygen species during early stages of ectomycorrhiza establishment between Castanea sativa and Pisolithus tinctorius. Mycorrhiza. ISSN 0940-6360. 17:3, p. 185-193
Publisher
Springer