Study of the mecanisms regulating the interactions between ectomycorrhizal fungi and roots of Castanea sativa Mill. European chestnut

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Efeito de extractos de raízes eliciadas de Castanea sativa no crescimento do fungo ectomicorrízico Pisolithus tinctorius

Publication . Baptista, Paula; Martins, Anabela; Tavares, Rui Manuel; Lino-Neto, Teresa

O castanheiro (Castanea sativa Mill.) estabelece associação com numerosas espécies de fungos ectomicorrízicos, estando descritos os efeitos benéficos para a planta após micorrização com Pisolithus tinctorius. Neste processo é essencial que ocorra a troca de sinais entre os simbiontes, para que seja reconhecida a sua compatibilidade e para que ocorra a formação dos órgãos ectomicorrízicos. Neste trabalho são fornecidas evidências que sugerem a capacidade de extractos de raízes de castanheiro, nos estádios iniciais de contacto com P. tinctorius regularem o crescimento do fungo micorrízico.

2007Conference object

Open access

Signalling process during the early contact of ectomycorrhizal fungi Pisolithus tinctorius with Castanea sativa roots

Publication . Baptista, Paula; Martins, Anabela; Tavares, Rui Manuel; Lino-Neto, Teresa

Ectomycorrhizas (ECM) are symbiotic associations between roots of higher plants, mainly trees (e.g. Pinacea and Fagaceae), and soil fungi (asco- and basidiomycetes). The establishment of this symbiotic association requires a sequence of highly regulated and coordinated events, initiated by an exchange of specific signalling compounds between both partners.

2006Conference object

Open access

Involvement of reactive oxygen species during early stages of ectomycorrhiza establishment between Castanea sativa and Pisolithus tinctorius

Publication . Baptista, Paula; Martins, Anabela; Pais, Maria Salomé; Tavares, Rui Manuel; Lino-Neto, Teresa

Evidence for the participation of reactive oxygen species (ROS) and antioxidant systems in ectomycorrhizal(ECM) establishment is lacking. In this paper, we evaluated ROS production and the activities of superoxide dismutase (SOD) and catalase (CAT) during the early contact of the ECM fungus Pisolithus tinctorius with the roots of Castanea sativa (chestnut tree). Roots were placed in contact with P. tinctorius mycelia, and ROS production was evaluated by determining the levels of H2O2 and O2 I− during the early stages of fungal contact. Three peaks of H2O2 production were detected, the first two coinciding with O2 I− bursts. The first H2O2 production peak coincided with an increase in SOD activity, whereas CAT activity seemed to be implicated in H2O2 scavenging. P. tinctorius growth was evaluated in the presence of P. tinctorius-elicited C. sativa crude extracts prepared during the early stages of fungal contact. Differential hyphal growth that matched the H2O2 production profile with a delay was detected. The result suggests that during theearly stages of ECM establishment, H2O2 results from an inhibition of ROS-scavenging enzymes and plays a role in signalling during symbiotic establishment.

2007Journal article

Open access