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Multidrug-Resistant Escherichia coli Accumulated by Freshwater Bivalves: An Underestimated Risk for Public Health?

2024Journal article Open access
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dc.contributor.authorMartins, Joana
dc.contributor.authorGonçalves, Ana
dc.contributor.authorFernandes, Conceição
dc.contributor.authorCabecinha, Edna
dc.contributor.authorMonteiro, Sandra Mariza
dc.contributor.authorGuedes, Hugo
dc.contributor.authorAlmeida, Gonçalo
dc.contributor.authorGarcia, Juliana
dc.contributor.authorSilva, Gabriela Jorge da
dc.contributor.authorVarandas, Simone
dc.contributor.authorSaavedra, Maria José
dc.date.accessioned2024-10-16T08:58:02Z
dc.date.available2024-10-16T08:58:02Z
dc.date.issued2024
dc.description.abstractAs bioindicators, freshwater bivalves are crucial for the assessment of the contamination impact on different levels of biological integration. Escherichia coli is used as a bioindicator of water fecal contamination, representing a critical global concern, especially with the rise of multidrugresistant (MDR) strains. Phylogenetic diversity, pathotypic characterization, and antibiotic resistance profiles of E. coli isolated from freshwater bivalves (Anodonta anatina) were assessed. Samples were collected from the Tua River in Northern Portugal, from two different sites, Chelas and Barcel, representing different degrees of contamination. Antimicrobial susceptibility testing was performed by the disk diffusion method, and characterizations of the phylogenetic groups and pathotypes were assessed by PCR-multiplex and real-time PCR-multiplex, respectively. Results showed that 60% of isolates were characterized as MDR, including resistance to carbapenems, considered the last resort against multidrug-resistant bacteria. Within this study, it was also possible to verify the antimicrobial resistance (AMR) profile differences between the two sampling sites, with bivalve isolates from the Chelas site showing a higher percentage of antibiotic resistance. Among the E. coli isolates, the highest prevalence (55%) was observed in group B1, followed by group D or E (15%), group A (10%), and group E or Clade I (10%). None of the isolates were classified as diarrheagenic E. coli (DEC). This work highlights the potential transmission of antimicrobial-resistant bacteria through bivalves in the food chain. The ‘One Health’ approach is crucial for combating antimicrobial resistance, namely in edible freshwater species, emphasizing active surveillance to protect human, animal, and environmental health against the spread of antibiotic-resistant bacteria in aquatic environments.pt_PT
dc.description.sponsorshipThis research was supported by the Portuguese Foundation for Science and Technology (FCT) and the Operational Competitiveness Programme (COMPETE), under the projects UIDB/ 04033/2020 (CITAB-UTAD), and Inov4Agro (LA/P/0126/2020); UIDB/00772/2020 (CECAV), UIDB/00211/2020 (CECA-ICETA), and AL4AnimalS (LA/P/0059/2020); UIDB/00690/2020 (CIMO), and LA/P/0007/2020 (SusTEC). Joana Martins (J.C.L.M.): Fundação para a Ciência e a Tecnologia (FCT) for her PhD Grant (2023.05610.BDANA).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMartins, Joana C. L.; Gonçalves, Ana; Fernandes, Conceição; Cabecinha, Edna; Monteiro, Sandra; Guedes, Hugo; Almeida, Gonçalo; Garcia, Juliana; Silva, Gabriela J. da; Varandas, Simone; Saavedra, Maria J. (2024). Multidrug-Resistant Escherichia coli Accumulated by Freshwater Bivalves: An Underestimated Risk for Public Health?. Pathogens. ISSN 2076-0817. 13:8, p. 1-13pt_PT
dc.identifier.doi10.3390/pathogens13080617pt_PT
dc.identifier.issn2076-0817
dc.identifier.urihttp://hdl.handle.net/10198/30431
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationCentre for the Research and Technology of Agro-Environmental and Biological Sciences
dc.relationInstitute for innovation, capacity building and sustainability of agri-food production
dc.relationVeterinary and Animal Science Research Centre
dc.relationCenter for the Study of Animal Science
dc.relationAssociate Laboratory for Animal and Veterinary Sciences
dc.relationMountain Research Center
dc.relationAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnodonta anatinapt_PT
dc.subjectAntimicrobial resistancept_PT
dc.subjectOne healthpt_PT
dc.subjectPhylogeneticpt_PT
dc.subjectAnimal healthpt_PT
dc.titleMultidrug-Resistant Escherichia coli Accumulated by Freshwater Bivalves: An Underestimated Risk for Public Health?pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for the Research and Technology of Agro-Environmental and Biological Sciences
oaire.awardTitleInstitute for innovation, capacity building and sustainability of agri-food production
oaire.awardTitleVeterinary and Animal Science Research Centre
oaire.awardTitleCenter for the Study of Animal Science
oaire.awardTitleAssociate Laboratory for Animal and Veterinary Sciences
oaire.awardTitleMountain Research Center
oaire.awardTitleAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
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oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0007%2F2020/PT
oaire.citation.endPage13pt_PT
oaire.citation.issue8pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titlePathogenspt_PT
oaire.citation.volume13pt_PT
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person.familyNameFernandes
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person.identifier.ciencia-id6514-3D41-4534
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rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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