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In vitro and in vivo evaluation of enzymatic and antioxidant activity, cytotoxicity and genotoxicity of curcumin-loaded solid dispersions

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dc.contributor.authorSilva, Igor
dc.contributor.authorPeron, Ana Paula
dc.contributor.authorLeimann, Fernanda Vitória
dc.contributor.authorBressan, Getúlio Nicola
dc.contributor.authorKrum, Bárbara Nunes
dc.contributor.authorFachinetto, Roselei
dc.contributor.authorPinela, José
dc.contributor.authorCalhelha, Ricardo C.
dc.contributor.authorBarreiro, M.F.
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorGonçalves, Odinei Hess
dc.contributor.authorIneu, Rafael P.
dc.date.accessioned2018-02-19T10:00:00Z
dc.date.accessioned2019-02-04T17:10:17Z
dc.date.available2018-01-19T10:00:00Z
dc.date.available2019-02-04T17:10:17Z
dc.date.issued2019
dc.description.abstractCurcumin, the main bioactive polyphenolic compound in Curcuma longa L. rhizomes has a wide range of bioactive properties. Curcumin presents low solubility in water and thus limited bioavailability, which decreases its applicability. In this study, cytotoxic effects of curcumin solid dispersions (CurSD) were evaluated against tumor (breast adenocarcinoma and lung, cervical and hepatocellular carcinoma) and non-tumor (PLP2) cells, while cytotoxic and genotoxic effects were evaluated in Allium cepa. The effect of the CurSD on the acetylcholinesterase (AChE), butyrylcholinesterase (BChE), glutathione S-transferase (GST), andmonoamine oxidase (MAO A-B) enzymes was determined, as well as its capacity to inhibit the oxidative hemolysis (OxHLIA) and the formation of thiobarbituric acid reactive substances (TBARS). CurSD are constituted by nanoparticles that are readily dispersible in water, and inhibited 24% and 64% of the AChE and BChE activity at 100μM, respectively. GST activity was inhibited at 30μM while MAO-A and B activity were inhibited at 100μM. CurSD showed cytotoxicity against all the tested tumor cell lines without toxic effects for non-tumor cells. No cytotoxic and genotoxic potential was detected with the Allium cepa test.CurSD maintained the characteristics of free curcumin on the in vitro modulation of important enzymes without appreciable toxicity.en_EN
dc.description.sponsorshipThe authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme PT2020 for financial support to CIMO (strategic project UID/AGR/00690/2013) and the research contracts of J. Pinela (Project AllNatt, POCI-01-0145- FEDER-030463) and R. Calhelha. To the project POCI-01-0145-FEDER- 006984 – Associate Laboratory LSRE-LCM funded by FEDER through COMPETE2020 - Programa Operacional Competitividade e Internacionalização (POCI) – and by national funds through FCT. This work was also funded by the European Regional Development Fund through the Regional Operational Program North 2020, within the scope of Project NORTE-01-0145-FEDER-023289: DeCodE and Project Mobilizador Norte-01-0247-FEDER-024479: ValorNatural®.
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationSilva, Igor; Peron, Ana Paula; Leimann, Fernanda Vitória; Bressan, Getúlio Nicola; Krum, Bárbara Nunes; Fachinetto, Roselei; Pinela, José; Calhelha, Ricardo C.; Barreiro, M.F.; Ferreira, Isabel C.F.R.; Gonçalves, Odinei Hess; Ineu, Rafael Porto (2019). In vitro and in vivo evaluation of enzymatic and antioxidant activity, cytotoxicity and genotoxicity of curcumin-loaded solid dispersions. Food and Chemical Toxicology. ISSN 0278-6915. 125, p. 29-37en_EN
dc.identifier.doi10.1016/j.fct.2018.12.037en_EN
dc.identifier.issn0278-6915
dc.identifier.urihttp://hdl.handle.net/10198/18731
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.relationNORTE-01-0145-FEDER-023289
dc.relationNorte-01-0247-FEDER-024479
dc.relationPOCI-01-0145-FEDER-030463
dc.subjectAllium cepaen_EN
dc.subjectBioactivityen_EN
dc.subjectCholinesteraseen_EN
dc.subjectMonoamine oxidaseen_EN
dc.subjectNanoparticlesen_EN
dc.subjectPoloxamer 407en_EN
dc.subjectSolid dispersionen_EN
dc.titleIn vitro and in vivo evaluation of enzymatic and antioxidant activity, cytotoxicity and genotoxicity of curcumin-loaded solid dispersionsen_EN
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAGR%2F00690%2F2013/PT
oaire.fundingStream5876
person.familyNameLeimann
person.familyNamePinela
person.familyNameCalhelha
person.familyNameBarreiro
person.familyNameFerreira
person.familyNameGonçalves
person.givenNameFernanda Vitória
person.givenNameJosé
person.givenNameRicardo C.
person.givenNameFilomena
person.givenNameIsabel C.F.R.
person.givenNameOdinei Hess
person.identifier144781
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person.identifier.ciencia-id771C-2B43-B108
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person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0001-6230-9597
person.identifier.orcid0000-0001-7523-1637
person.identifier.orcid0000-0002-6801-4578
person.identifier.orcid0000-0002-6844-333X
person.identifier.orcid0000-0003-4910-4882
person.identifier.orcid0000-0002-9528-8187
person.identifier.ridB-4466-2014
person.identifier.ridJ-2172-2014
person.identifier.ridL-9802-2014
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id54392272800
person.identifier.scopus-author-id6507978333
person.identifier.scopus-author-id57188719717
person.identifier.scopus-author-id36868826600
person.identifier.scopus-author-id9335958400
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
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