| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 561.65 KB | Adobe PDF |
Advisor(s)
Abstract(s)
The Apis mellifera iberiensis was introduced to the Azores in the 16th century by Portuguese settlers likely from the northern part of mainland Portugal. Many years later, in the 1980s, occurred a new introduction using honey bee subspecies from C-lineage maternal ancestry. This left a C-lineage signature in the Azores, with some islands, such as Pico or Graciosa, showing high frequency of C-lineage maternal ancestry as revealed by the study conducted in 2014/2015 (Ferreira et al. 2020). Hence, this study aimed to evaluate the C-lineage maternal signature on São Miguel, seven years later (samples collected in 2023). A total of 192 colonies were sampled across the island and the total DNA was extracted from a pool of fifteen right front legs, were removed and placed in a 2.0-mL screwcap tube containing two zirconia beads of 2 mm diameter. The DNA extracts were then amplified using PCR targeting the tRNAleu-cox2 region of the mitochondrial DNA, followed by Sanger sequencing. The results show a prevalence of 64% for lineage A, contrasted with the 70% recorded in 2014/2015. Additionally, lineage C had a prevalence of 20%, compared to 27% in 2014/2015. In the A-lineage, the most prevalent haplotype was A14, while in the C-lineage, it was the C2 haplotype. Consequently, it can be inferred even almost 45 years after the C-lineage ancestry introduction, there are still a considerable frequency of this foreign maternal origin.
Description
Keywords
Apis mellifera Genetic diversity Maternal lineage mtDNA Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology
Citation
Costa, Maíra; Lopes, Ana; Doblas-Bajo, Mónica; Quaresma, Andreia; Barreira, Diogo; Henriques, Dora; Pinto, M. Alice (2024). Maternal ancestry of honey bees on São Miguel-Azores (Portugal). In Third International Congress on Bee Sciences. Online. ISBN 978-605-71368-8-6
Publisher
Ulas Acaröz