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Abstract(s)
Sendo a raça ovina Churra Galega Bragançana autóctone do nordeste de Portugal, encontra-se bem adaptada ao seu Solar, porém algumas condições ambientais são bastante adversas fazendo com que muitas vezes estes animais não expressem o seu potencial produtivo. Também é do nosso conhecimento que pequenas mudanças quer no maneio quer na alimentação podem contribuír para um melhor desempenho destes animais. Nesse sentido, surgiu a ideia de aproveitar o colostro de vaca como suplemento de cordeiros, visto que este é excedentário e muitas vezes descartado nas explorações intensivas de bovinos de leite. Neste ensaio utilizaram-se 18 ovinos da raça Churra Galega Bragançana os quais foram divididos em dois grupos: o controlo e o grupo tratamento (3 ovelhas e 6 cordeiros, cada grupo). Os cordeiros do grupo tratamento, a partir do terceiro dia de idade foram suplementados com 50ml pôr dia de colostro de duas vacas Holstein-Frísia, durante 4 semanas. Os cordeiros foram pesados aos 10, 17, 24 e 33 dias de idade. A comunidade bacteriana presente nas amostras de fezes de cordeiros, colostro de vaca e leite das mães foi avaliada recorrendo a métodos não dependentes do cultivo utilizando a plataforma Illumina miseq. Nas amostras de fezes, a diversidade bacteriana foi avaliada adicionalmente por métodos dependentes de cultivo, com o intuito de avaliar nos isolados obtidos a capacidade produtiva de enzimas líticas. Relativamente ao ganho médio diário (GMD) dos cordeiros, no período dos 10 dias após o nascimento, o grupo tratamento mostrou um GMD (0,232g/d) significativamente maior (p=0,031) que o controlo (0,127g/d), o mesmo não se verificando nos diferentes períodos. No total, os dois métodos permitiram identificar 93 géneros nas fezes e de 231 géneros nas amostras lácteas, sendo Bacteroides e Acinetobacter os géneros mais abundantes na comunidade bacteriana, respetivamente.Verificou-se que a composição da comunidade bacteriana das fezes dos cordeiros controlo é significativamente diferente (R=0,48; p=0,029) da comunidade presente nas fezes dos cordeiros que ingeriram colostro de vaca. Dos isolados bacterianos obtidos das fezes, por métodos dependentes de cultivo foram identificados três OTUs (Acinetobacter sp., Jeotgalicoccus sp. e Bacillus sp.) que mostraram capacidade em produzir enzimas digestivas, tais como amílase, celulase, lípase e protéase. Desta forma, estes isolados apresentam um potencial enorme na nutrição animal e adicionalmente como adjuvantes imunológicos e/ou probiótico. Apesar dos resultados obtidos serem bastante promissores dever-se-ão realizar mais estudos.
The autochthonous sheep breed Churra Galega Bragançana from the northeast of Portugal, it is well adapted to its habitat, but some environmental conditions are quite adverse, often causing these animals not express their productive potential. We also know that small changes in both management and feeding can contribute to a better improvement of these animals. In this sense, the idea arose to use cow colostrum as a supplement for lambs, as it is surplus and often discarded in intensive dairy cattle farms. In this study 18 of the Churra Galega Bragançana sheep breed were used and divided into two groups: the control and the treatment group (3 ewes and 6 lambs each). The lambs of the treatment group, from the third day of age were supplemented with 50 ml per day of colostrum from two Holstein-Friesian cows, for 4 weeks. The lambs were weighed at 10, 17, 24 and 33 days of age. The bacterial community present in the faeces samples of lambs, cow colostrum and milk from their mothers was evaluated using non-cultivation methods using the Illumina miseq platform. In stool samples, bacterial diversity was additionally evaluated by culture-dependent methods in order to evaluate the lytic enzyme production capacity of the isolates obtained. Regarding the average daily gain (ADG) of lambs, in the period of 10 days after birth, the treatment group showed a ADG (0.232g/d) significantly higher (p= 0.031) than the control group (0.127g/d), the same not occurring in the different periods. In total, the two methods identified 93 genera in faeces and 231 genera in milk samples, with Bacteroids and Acinetobacter being the most abundant genera in the bacterial community respectively. It was found that the composition of the bacterial community of the control lambs' faeces is significantly different (R=0.48; p=0.029) from the community present in the faeces of lambs that ingested cow colostrum. From the bacterial isolates obtained from feces, three OTUs (Acinetobacter sp., Jeotgalicoccus sp. and Bacillus sp.) were identified by cultivation-dependent methods that showed capacity to produce digestive enzymes such as amylase, cellulase, lipase and proteinase. In this way, these isolates present an enormous potential in animal nutrition and additionally as immunological and/or probiotic adjuvants. Although the results obtained are quite promising, further studies should be carried out.
The autochthonous sheep breed Churra Galega Bragançana from the northeast of Portugal, it is well adapted to its habitat, but some environmental conditions are quite adverse, often causing these animals not express their productive potential. We also know that small changes in both management and feeding can contribute to a better improvement of these animals. In this sense, the idea arose to use cow colostrum as a supplement for lambs, as it is surplus and often discarded in intensive dairy cattle farms. In this study 18 of the Churra Galega Bragançana sheep breed were used and divided into two groups: the control and the treatment group (3 ewes and 6 lambs each). The lambs of the treatment group, from the third day of age were supplemented with 50 ml per day of colostrum from two Holstein-Friesian cows, for 4 weeks. The lambs were weighed at 10, 17, 24 and 33 days of age. The bacterial community present in the faeces samples of lambs, cow colostrum and milk from their mothers was evaluated using non-cultivation methods using the Illumina miseq platform. In stool samples, bacterial diversity was additionally evaluated by culture-dependent methods in order to evaluate the lytic enzyme production capacity of the isolates obtained. Regarding the average daily gain (ADG) of lambs, in the period of 10 days after birth, the treatment group showed a ADG (0.232g/d) significantly higher (p= 0.031) than the control group (0.127g/d), the same not occurring in the different periods. In total, the two methods identified 93 genera in faeces and 231 genera in milk samples, with Bacteroids and Acinetobacter being the most abundant genera in the bacterial community respectively. It was found that the composition of the bacterial community of the control lambs' faeces is significantly different (R=0.48; p=0.029) from the community present in the faeces of lambs that ingested cow colostrum. From the bacterial isolates obtained from feces, three OTUs (Acinetobacter sp., Jeotgalicoccus sp. and Bacillus sp.) were identified by cultivation-dependent methods that showed capacity to produce digestive enzymes such as amylase, cellulase, lipase and proteinase. In this way, these isolates present an enormous potential in animal nutrition and additionally as immunological and/or probiotic adjuvants. Although the results obtained are quite promising, further studies should be carried out.
Description
Dupla diplomação com a Universidade Tecnológica Federal do Paraná
Keywords
Ovinos Composição microbiana Colostro Enzimas Fezes