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Effects of high cell density fermentations on the optimisation of mead fermentation

dc.contributor.authorPereira, Ana Paula
dc.contributor.authorFerreira, Alexandra Mendes
dc.contributor.authorEstevinho, Leticia M.
dc.contributor.authorFaia, Arlete Mendes
dc.date.accessioned2014-08-12T11:13:47Z
dc.date.available2014-08-12T11:13:47Z
dc.date.issued2012
dc.description.abstractMead is a traditional drink, containing 8-18% (v/v) of ethanol, which results from the alcoholic fermentation of diluted honey performed by yeasts. It has been reported that mead fermentation is a time-consuming process, often taking several months to complete, depending on the type of honey, yeast strain and honey-must’s composition. An important objective of mead makers is to reduce the fermentation time without decreasing the quality of the end product. It has been shown that significant time can be saved in the fermentation process by increasing the pitching rate, i.e., the amount of suspended yeast cells added to a batch fermenter. Therefore, the aim of this study was to determine the adequate inoculum size of two commercial winemaking strains of Saccharomyces cerevisiae (Lalvin QA23 and Lalvin ICV D47) for the optimisation of mead fermentation. Honey must was prepared according to the recipe developed by our team, supplemented with potassium tartrate, pH adjusted to 3.7 with malic acid and the nitrogen concentration adjusted to 267 mg/L with diammonium phosphate. The appropriate amounts of inoculum were pitched into the honey-must to obtain five different pitching rates. Several parameters were determined during the fermentation to evaluate the effect of the inoculum size on yeast growth, fermentation profile and mead composition. Minor differences between the two strains in respect to growth kinetics were detected. As expected the increasing of the inoculum size resulted in significant increases in cell biomass and CFUs’ numbers but also decreased the yeast net growth. The time required to reach the same stage of fermentation ranged from 24 to 96 hours depending on the inoculum size. In accordance to the results obtained the strain ICV D47 appears to be more suitable for the production of high quality meads, although the strain QA23 provided better fermentation profile. However, sugars were not fully consumed and about 25 mg/L of assimilable nitrogen remained at the end of all fermentations. This is the first study of the effect of inoculum size on the optimisation of mead fermentation, however further research is needed to improve its quality.por
dc.identifier.citationPereira, Ana Paula; Ferreira, Alexandra Mendes; Estevinho, Leticia M.; Faia, Arlete Mendes (2012). Effects of high cell density fermentations on the optimisation of mead fermentation. In II International Symposium on Bee Products. Annual Meeting of IHC: book of abstracts. Bragança. ISBN 987-972-745-140-1por
dc.identifier.isbn987-972-745-140-1
dc.identifier.urihttp://hdl.handle.net/10198/10105
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherInstituto Politécnico de Bragançapor
dc.titleEffects of high cell density fermentations on the optimisation of mead fermentationpor
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceBragança, Portugalpor
oaire.citation.titleII International Symposium on Bee Product. Annual Meeting of the International Honey Comissionpor
person.familyNamePereira
person.familyNameEstevinho
person.givenNameAna Paula
person.givenNameLetícia M.
person.identifier.ciencia-id1413-70AA-CDF6
person.identifier.ciencia-idBA14-09D6-A406
person.identifier.orcid0000-0003-1489-5207
person.identifier.orcid0000-0002-9249-1948
person.identifier.scopus-author-id54401473200
person.identifier.scopus-author-id6506577664
rcaap.rightsopenAccesspor
rcaap.typeconferenceObjectpor
relation.isAuthorOfPublicatione7df6eac-d667-415d-abdb-9fe17467475c
relation.isAuthorOfPublication4a1d5ba2-1854-4ca5-89a4-73f35e964df9
relation.isAuthorOfPublication.latestForDiscovery4a1d5ba2-1854-4ca5-89a4-73f35e964df9

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