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Comparison between different extraction methods in the recovery of bioactive molecules from Melissa officinalis L. under sustainable cultivation: chemical and bioactive characterization

2022Conference object Open access
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dc.contributor.authorOliveira, Izamara
dc.contributor.authorHeleno, Sandrina A.
dc.contributor.authorCarocho, Márcio
dc.contributor.authorAlves, Maria José
dc.contributor.authorVaz, Josiana A.
dc.contributor.authorDias, Maria Inês
dc.contributor.authorBuelga, Celestino S.
dc.contributor.authorPetropoulos, Spyridon Α.
dc.contributor.authorTzortzakis, Nikolaos
dc.contributor.authorChrysargyris, Antonios
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorBarros, Lillian
dc.date.accessioned2023-02-20T15:55:15Z
dc.date.available2023-02-20T15:55:15Z
dc.date.issued2022
dc.description.abstractMelissa officinalis L., from the Lamiaceae family, is one of the most important medicinal and aromatic plants with potential in the market. With the passing of time, the use of medicinal plants in the treatment of some illness has gone from the simplest forms of local treatment to the industrial manufacture of phytotherapics. In addition to their medicinal effect, they can also be used in the form of infusions and decoctions and in various food preparations. In this sense, the objective of this work was to compare three different extraction methods: infusion (100% water), maceration (80:20 ethanol: water v:v) and ultrasound-assisted extraction (UAE) under previous optimized extraction conditions (33.0 +- 3.2 min, 371.7 +- 19.3Wand 39.9 +- 1.4% ethanol), in plants grown under sustainable cultivation under full irrigation in June. The parameters studied included bioactive evaluation by antioxidant (thiobarbituric acid reactive substances—TBARS), cytotoxicity (sulforhodamine B) and anti-inflammatory (RAWcells) assays. The composition of phenolic compounds and organic acids was also studied by GC-MS and HPLC-DAD, respectively. According to the obtained results, eight phenolic compounds were identified and quantified, being rosmarinic acid the main one for the three extraction methods (infusion: 107.1 +- 0.9 mg/g extract; maceration: 155.7 +- 0.3 mg/g extract; UAE: 118.7 0.6 mg/g extract). For Lithospermic acid A isomer (25.25 +- 0.01 mg/g) and Hydroxylsalvianolic E (111.70 +- 2.20 mg/g), the UAE revealed the lowest content of individual polyphenols, whereas maceration recorded the highest extractability. On the other hand, the content of six of the eight polyphenols detected for the ultrasound-assisted extraction was similar to the infusion and maceration methods. In terms of antioxidant activity, the infusions showed the highest capacity (3.00 +- 0.14 +-g/mL), followed by maceration (5.33 +- 0.30 +-g/mL) and UAE (12 +- 0.15 +-g/mL). The highest anti-inflammatory activity was verified for the infusion (244 +- 11 +-g/mL), followed by UAE (305 +- 9 +-g/mL), with no activity recorded for the maceration extract (>400 +-g/mL). The antitumor properties were evaluated in five cell lines, with the best results being recorded for infusion, except AGS (24 +- 1 +-g/mL) wherept_PT
dc.description.sponsorshipThe authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES to CIMO (UIDB/00690/2020). L. Barros and M.I. Dias thank the national funding by FCT through the institutional scientific employment program-contract for her contract, while M. Carocho and S. Heleno thank FCT through the indiviual scientific employment program-contracts (CEECIND/00831/2018 and CEECIND/03040/2017). I. Oliveira thanks FCT for her PhD grant (BD/06017/2020). To FEDER-Interreg España-Portugal programme for financial support through the project TRANSCoLAB 0612_TRANS_CO_LAB_2_P; to ERDF through the Regional Operational ProgramNorth 2020, within the scope of Project GreenHealth— Norte-01-0145-FEDER-000042.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationOliveira, Izamara de; Heleno, Sandrina A.; Carocho, Márcio; Alves, Maria José; Vaz, Josiana; Dias, Maria Inês; Buelga, Celestino S.; Petropoulos, Spyridon; Tzortzakis, Nikolaos; Chrysargyris, Antonios; Ferreira, Isabel C.F.R.; Barros, Lillian (2022). Comparison between different extraction methods in the recovery of bioactive molecules from Melissa officinalis L. under sustainable cultivation: chemical and bioactive characterization. Biology and Life Sciences Forum. ISSN 2673-9976. 11:1, p. 1-2pt_PT
dc.identifier.doi10.3390/IECPS2021-12020pt_PT
dc.identifier.issn2673-9976
dc.identifier.urihttp://hdl.handle.net/10198/27069
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationBD/06017/2020pt_PT
dc.relationMountain Research Center
dc.relationNot Available
dc.relationNot Available
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMelissa officinalis L.pt_PT
dc.subjectDifferent extraction methodspt_PT
dc.subjectPhenolic compoundspt_PT
dc.titleComparison between different extraction methods in the recovery of bioactive molecules from Melissa officinalis L. under sustainable cultivation: chemical and bioactive characterizationpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardTitleNot Available
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2018/CEECIND%2F00831%2F2018%2FCP1578%2FCT0001/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F03040%2F2017%2FCP1403%2FCT0001/PT
oaire.citation.endPage56pt_PT
oaire.citation.startPage55pt_PT
oaire.citation.titleBiology and Life Sciences Forumpt_PT
oaire.citation.volume11pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamCEEC IND 2018
oaire.fundingStreamCEEC IND 2017
person.familyNameOliveira
person.familyNameHeleno
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person.givenNameIzamara
person.givenNameSandrina A.
person.givenNameMárcio
person.givenNameMaria José
person.givenNameJosiana A.
person.givenNameMaria Inês
person.givenNameIsabel C.F.R.
person.givenNameLillian
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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