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Antioxidants in Pinus Pinaster and mycorrhizal fungi during the early steps of symbiosis

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dc.contributor.authorCarocho, Márcio
dc.contributor.authorBarros, Lillian
dc.contributor.authorBarreira, João C.M.
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorMartins, Anabela
dc.date.accessioned2012-08-17T10:21:56Z
dc.date.available2012-08-17T10:21:56Z
dc.date.issued2012
dc.description.abstractEctomycorrhizal symbiosis between fungi and the roots of some plants could have important effects in t he levels of antioxidants of both partners [1,2]. In the present work, the effects of eo-culture period (6, 24 and 72 h) in the antioxidant properties and antioxidants production duri ng early steps of in vitro mycorrhization (Pinus pinaster - Pisolithus arhizus and Pinus pinaster - Paxillus involutus) were evaluated. The studied parameters were determined in each culture element (root, mycelium and medium) in order to understand the response of each part ner to the symbiot ic association. The antioxidant properties were determined by assays evaluating free radicals scavenging activi ty, reducing power, ~-ca rotene bleaching inhibition and lipid peroxidation inhibition in animal brain homogenates. The concent rations in phenolics, tocopherols and suga rs were obtained by Folin Ciocalteus assay, HPLC-fluorescence and HPLC-RI, respectively. P. arhizus proved to be more compatible with P. pinaster than P. involutus, since the antioxidant activity in the latter species increased from 48 to 72 h, while P. arhizus antioxidant properties decreased at the same period (less oxidative stress). Despite P. involutus proved to be less suitable to be included in forestation programs using mycorrhization processes, it revealed a higher potential for bioactive compounds production in the early steps of symbiosis. A maximal phenolics content was obtained after the first 6 h and, considering bioactive compounds production purposes, the eoculture should be stopped at t hat time. Tocopherols (except y-tocopherol) contents showed high similarities among culture medium and mycelia. This tendency could not be observed regarding sugars contents, which might indicate that the differences in t he chemical responses that characterize each mycorrhization might be related more directly with sugar (primary metabolites) exchanges.por
dc.identifier.citationCarocho, Márcio; Barros, Lillian; Barreira, João C.M.; Ferreira, Isabel C.F.R.; Martins, Anabela (2012). Antioxidants in Pinus Pinaster and mycorrhizal fungi during the early steps of symbiosis. In 7º Encontro Nacional de Cromatografia. Portopor
dc.identifier.urihttp://hdl.handle.net/10198/7352
dc.language.isoengpor
dc.peerreviewedyespor
dc.relationBPD/4609/2008
dc.relationPortuguese Wild Mushrooms: Chemical characterization and functional study of antiproliferative and proapoptotic properties in cancer cell lines
dc.relationBIOACTIVE PROPERTIES & CYTOPROTECTIVE POTENTIAL OF NATURAL EXTRACTS/INDIVIDUAL COMPOUNDS: APPLICATION OF SINGLE CELL GEL ELECTROPHORESIS AND OTHER BIOCHEMICAL, CHEMICAL AND ELECTROCHEMICAL ASSAYS
dc.titleAntioxidants in Pinus Pinaster and mycorrhizal fungi during the early steps of symbiosispor
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitlePortuguese Wild Mushrooms: Chemical characterization and functional study of antiproliferative and proapoptotic properties in cancer cell lines
oaire.awardTitleBIOACTIVE PROPERTIES & CYTOPROTECTIVE POTENTIAL OF NATURAL EXTRACTS/INDIVIDUAL COMPOUNDS: APPLICATION OF SINGLE CELL GEL ELECTROPHORESIS AND OTHER BIOCHEMICAL, CHEMICAL AND ELECTROCHEMICAL ASSAYS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FAGR-ALI%2F110062%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F72802%2F2010/PT
oaire.citation.conferencePlacePortopor
oaire.citation.title7º Encontro Nacional de Cromatografia, 9 a 11 de Janeiro de 2012por
oaire.fundingStream5876-PPCDTI
person.familyNameCarocho
person.familyNameBarros
person.familyNameBarreira
person.familyNameFerreira
person.familyNameMartins
person.givenNameMárcio
person.givenNameLillian
person.givenNameJoão C.M.
person.givenNameIsabel C.F.R.
person.givenNameAnabela
person.identifierawbWiiMAAAAJ&hl
person.identifier469085
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person.identifier.orcid0000-0002-8978-4547
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0003-1233-0990
person.identifier.orcid0000-0003-4910-4882
person.identifier.orcid0000-0001-6218-4413
person.identifier.ridJ-3600-2013
person.identifier.ridD-8269-2013
person.identifier.ridE-8500-2013
person.identifier.ridG-5488-2013
person.identifier.scopus-author-id52463229800
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id54895546900
person.identifier.scopus-author-id36868826600
person.identifier.scopus-author-id7203013518
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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