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Effect of extraction method on the bioactive composition, antimicrobial activity and phytotoxicity of pomegranate by-products

2022Journal article Open access
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dc.contributor.authorCampos, Lara
dc.contributor.authorSeixas, Luana
dc.contributor.authorDias, Susana
dc.contributor.authorPeres, António M.
dc.contributor.authorVeloso, Ana C.A.
dc.contributor.authorHenriques, Marta H.F.
dc.date.accessioned2022-05-18T10:31:43Z
dc.date.available2022-05-18T10:31:43Z
dc.date.issued2022
dc.description.abstractPomegranate by-products can be an asset to the food industry due to the richness in bioactive and antimicrobial compounds. This work studied the influence of conventional solvent and sonication-assisted extraction methods on the bioactive profile, antimicrobial properties, and phytotoxicity effect of the peels and seeds extracts from Acco, Big Full, and Wonderful pomegranate cultivars. The bioactive composition of the extracts was evaluated for the content of total phenolics, total flavonoids, and antioxidant activity (expressed as the half-maximal inhibitory concentration— IC50) by spectrophotometric methods, while the tannins were determined by titration and the anthocyanins were estimated by the pH-differential method. For the evaluation of the antimicrobial activity, the disk diffusion method of Kirby-Bauer was adapted through inhibition halos against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, and Yarrowia lipolytica. The extracts’ phytotoxicity was evaluated in vitro on garden-cress seeds. Extracts from conventional extraction were richer in total phenolics, expressed as gallic acid equivalents (0.16–0.73 mg GAE/mg extract), while those from sonication-assisted extraction had higher contents of total flavonoids, expressed as catechin equivalents (0.019–0.068 mg CATE/mg extract); anthocyanins, expressed as cyanidin-3-glucoside (0.06–0.60 g C3G/mg, dry basis); and antioxidant activity (IC50, 0.01–0.20 mg/mL). All extracts were more effective against Gram-positive bacteria and yeasts than Gram-negative bacteria. In general, the sonication-assisted extracts led to higher inhibition halos (8.7 to 11.4 mm). All extracts presented phytotoxicity against garden-cress seeds in the tested concentrations. Only the lowest concentration (0.003 mg/mL) enabled the germination of seeds and root growth, and the sonication-assisted extracts showed the highest Munoo-Liisa vitality index (51.3%). Overall, sonication-assisted extraction obtained extracts with greater bioactive and antimicrobial potential and less phytotoxicity.pt_PT
dc.description.sponsorshipThis work was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of CEB (UIDB/04469/2020), CERNAS (UIDB/00681/2020), CIMO (UIDB/00690/2020), and the Associate Laboratory SusTEC (LA/P/0007/2020). The European Regional Development Fund funded MobFood operation (LISBOA-01-0247-FEDER-024524). L.C. acknowledges research grants CEB-BI-14-2019 and FCT-IPC-i2A-CERNAS/Escola de Verão/BI-01-08, and L.S. acknowledges the research grant FCT-IPC-i2A-CERNAS/Escola de Verão/BII-01-07, all provided by FCT. The Article Processing Charge (APC) was funded by the Polytechnic Institute of Coimbra.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCampos, Lara; Seixas, Luana; Dias, Susana; Peres, António M.; Veloso, Ana C.A.; Henriques, Marta (2022). Effect of extraction method on the bioactive composition, antimicrobial activity and phytotoxicity of pomegranate by-products. Foods. EISSN 2304-8158. 11:7, p. 1-18pt_PT
dc.identifier.doi10.3390/foods11070992pt_PT
dc.identifier.eissn2304-8158
dc.identifier.urihttp://hdl.handle.net/10198/25479
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationLA/P/0007/2020pt_PT
dc.relationCentre of Biological Engineering of the University of Minho
dc.relationResearch Center in Natural Resources, Environment and Society
dc.relationMountain Research Center
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectSonication-assisted extractionpt_PT
dc.subjectSolvent extractionpt_PT
dc.subjectPunica granatum L.pt_PT
dc.subjectPomegranate peelspt_PT
dc.subjectPomegranate seedspt_PT
dc.subjectAntimicrobial activitypt_PT
dc.subjectPhytotoxicitypt_PT
dc.titleEffect of extraction method on the bioactive composition, antimicrobial activity and phytotoxicity of pomegranate by-productspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre of Biological Engineering of the University of Minho
oaire.awardTitleResearch Center in Natural Resources, Environment and Society
oaire.awardTitleMountain Research Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00681%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.citation.issue7pt_PT
oaire.citation.startPage992pt_PT
oaire.citation.titleFoodspt_PT
oaire.citation.volume11pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNamePeres
person.givenNameAntónio M.
person.identifier107333
person.identifier.ciencia-idCF16-5443-F420
person.identifier.orcid0000-0001-6595-9165
person.identifier.ridI-8470-2012
person.identifier.scopus-author-id7102331969
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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