Repository logo
 
Publication

Mushroom extract increases p53 expression and causes cell cycle arrest and apoptosis in a breast cancer cell line

2012Conference paper Open access
Simple item page
dc.contributor.authorVaz, Josiana A.
dc.contributor.authorTavares, Catarina
dc.contributor.authorAlmeida, Gabriela M.
dc.contributor.authorMartins, Anabela
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorVasconcelos, M. Helena
dc.date.accessioned2012-08-20T14:29:20Z
dc.date.available2012-08-20T14:29:20Z
dc.date.issued2012
dc.description.abstractMushrooms are part of the sexual life cycle of particular fungi with specific metabolic pathways, and therefore may contain a largely unexploited source of powerful new pharmaceutical products with potential antitumor properties [1,2]. Furthermore, they may have potential as functional foods. Suillus collinitus is an edible mushroom found in European pine forests. The aim of this work was to study the cytotoxic potential of extracts from this mushroom in various cancer cell lines. Different extracts (methanolic, ethanolic and aqueous) were prepared and extractinduced cell growth inhibition was assessed with the sulforhodamine B assay in four human tumour cell lines (lung, breast, colon and gastric cancer). The methanolic extract was further characterized in its phenolic composition by HPLC-DAD. The effects of the extract on cell cycle profile and apoptosis were evaluated by flow cytometry and the effect on the expression levels of proteins related to cell cycle and apoptosis was further investigated by Western blotting. Regarding cell growth inhibition, the methanolic extract was the most potent one, particularly in MCF-7 cells (GI50 25.2±0.2lg/ml). Moreover, the GI50 concentration ninduced a G1 cell cycle arrest, with a concomitant decrease in the percentage of cells in the S phase. Furthermore, it caused an increase in the percentage of apoptotic cells, from 6.0±0.2% in untreated cells, to 15.3±2.0% in treated cells. In addition, 48h treatment with the GI50 concentration caused a strong increase in the levels of p53, p21, cleaved caspase-3 and cleaved PARP, together with a decrease in Bcl-2. The main components identified in the methanolic extract were: protocatechuic acid (5.2±0.2mg/kg dw), p-hydroxybenzoic acid (14.1±1.2mg/kg) and cinnamic acid (1.3±0.2mg/kg). Results indicate that Suillus colinitus is a promising source of bioactive compounds. Particularly, its methanolic extract appears to have a p53-mediated effect on the normal cell cycle distribution and apoptosis induction in human breast tumor cells.por
dc.identifier.citationVaz, Josiana A.; Tavares, Catarina; Almeida, Gabriela M.; Martins, Anabela; Ferreira, Isabel C.F.R.; Vasconcelos, M. Helena (2012). Mushroom extract increases p53 expression and causes cell cycle arrest and apoptosis in a breast cancer cell line. In Targeted Anticancer Therapies. Amsterdam e Annals of Oncology. ISSN 0923-7534. 23:1, p. 28-29por
dc.identifier.urihttp://hdl.handle.net/10198/7360
dc.language.isoengpor
dc.peerreviewedyespor
dc.relationPortuguese Wild Mushrooms: Chemical characterization and functional study of antiproliferative and proapoptotic properties in cancer cell lines
dc.titleMushroom extract increases p53 expression and causes cell cycle arrest and apoptosis in a breast cancer cell linepor
dc.typeconference paper
dspace.entity.typePublication
oaire.awardTitlePortuguese Wild Mushrooms: Chemical characterization and functional study of antiproliferative and proapoptotic properties in cancer cell lines
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FAGR-ALI%2F110062%2F2009/PT
oaire.citation.conferencePlaceAmsterdam, Holandapor
oaire.citation.titleTargeted Anticancer Therapies 2012, 8 a 10 Março de 2012, Amsterdam, Holanda. P 2.10. Annals of Oncology, 23, Suppl. 1, i28.por
oaire.fundingStream5876-PPCDTI
person.familyNameVaz
person.familyNameMartins
person.familyNameFerreira
person.givenNameJosiana A.
person.givenNameAnabela
person.givenNameIsabel C.F.R.
person.identifier144781
person.identifier.ciencia-id171B-88BA-64F5
person.identifier.ciencia-id7B18-A810-6B93
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0002-6989-1169
person.identifier.orcid0000-0001-6218-4413
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridG-5488-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id16305323200
person.identifier.scopus-author-id7203013518
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typeconferenceObjectpor
relation.isAuthorOfPublication1c1a2cb9-5a36-47ba-bca0-ffcbef28df18
relation.isAuthorOfPublication383370dd-10e5-40e6-9a15-f2201fe95581
relation.isAuthorOfPublicationbd0d1537-2e03-41fb-b27a-140af9c35db8
relation.isAuthorOfPublication.latestForDiscovery383370dd-10e5-40e6-9a15-f2201fe95581
relation.isProjectOfPublication59db4b60-8cc2-4f07-a25a-8cfe78f2778f
relation.isProjectOfPublication.latestForDiscovery59db4b60-8cc2-4f07-a25a-8cfe78f2778f

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Poster Int. 58.pdf
Size:
101 KB
Format:
Adobe Portable Document Format
Description:
Documento principal
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CIMO - Publicações em Proceedings Indexadas à WoS/Scopus