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Unveiling the metabolites underlying the skin anti-ageing properties of Cytinus hypocistis (L.) L. through a biochemometric approach

2024Artigo científico Acesso aberto
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dc.contributor.authorSilva, Ana Rita
dc.contributor.authorAyuso, Manuel
dc.contributor.authorGarcía, Pablo A.
dc.contributor.authorBarros, Lillian
dc.contributor.authorEdrada-Ebel, RuAngelie
dc.date.accessioned2024-06-13T11:30:35Z
dc.date.available2024-06-13T11:30:35Z
dc.date.issued2024
dc.description.abstractThe genus Cytinus, recognised as one of the most enigmatic in the plant kingdom, has garnered attention for its bioactive potential, particularly its skin anti-ageing properties. Despite this recognition, much remains to be accomplished regarding deciphering and isolating its most active compounds. Hypothesis: This study aimed to identify the compounds responsible for C. hypocistis skin anti-ageing potential. Methods: Using multivariate analysis, a biochemometric approach was applied to identify the discriminant metabolites by integrating extracts' chemical profile (Liquid Chromatography–High-Resolution Mass Spectrometry, LC[sbnd]HRMS) and bioactive properties. The identified bioactive metabolite was structurally elucidated by 1D and 2D Nuclear Magnetic Resonance (NMR). Results: Among the studied bioactivities, the anti-elastase results exhibited a significant variation among the samples from different years. After the biochemometric analysis, the compound 2,3:4,6-bis(hexahydroxydiphenoyl)glucose, with a molecular mass of 784.075 Da, was structurally elucidated as the discriminant feature responsible for the outstanding human neutrophil elastase inhibition. Remarkably, the subfraction containing this compound exhibited a tenfold improvement in neutrophil elastase inhibition efficacy compared to the crude extract; its effectiveness fell within the same range as SPCK, a potent irreversible neutrophil elastase inhibitor. Moreover, this subfraction displayed no cytotoxicity or phototoxicity and excellent efficacy for the tested anti-ageing properties. Conclusions: Hydrolysable tannins were confirmed as the metabolites behind C. hypocistis skin anti-ageing properties, effectively mitigating critical molecular mechanisms that influence the phenotypically distinct ageing clinical manifestations. Pedunculagin was particularly effective in inhibiting neutrophil elastase, considered one of the most destructive enzymes in skin ageing.pt_PT
dc.description.sponsorshipThe authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES (PIDDAC) to CIMO (UIDB/00690/2020 and UIDP/00690/ 2020) and SusTEC (LA/P/0007/2020). A. R. Silva is grateful to FCT and FSE for her Doctoral Grant (SFRH/BD/145,834/2019), and L. Barros for her institutional scientific employment program-contract.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva, Ana Rita; Ayuso, Manuel; García, Pablo A.; Barros, Lillian; Edrada-Ebel, RuAngelie (2024). Unveiling the metabolites underlying the skin anti-ageing properties of Cytinus hypocistis (L.) L. through a biochemometric approach. Phytomedicine. ISSN 0944-7113. 129, p. 1-11pt_PT
dc.identifier.doi10.1016/j.phymed.2024.155685pt_PT
dc.identifier.issn0944-7113
dc.identifier.urihttp://hdl.handle.net/10198/29902
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationMountain Research Center
dc.relationMountain Research Center
dc.relationAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEllagitanninspt_PT
dc.subjectMetabolomicspt_PT
dc.subjectMultivariate statistical analysispt_PT
dc.subjectNeutrophil elastasept_PT
dc.titleUnveiling the metabolites underlying the skin anti-ageing properties of Cytinus hypocistis (L.) L. through a biochemometric approachpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberUIDB/00690/2020
oaire.awardNumberUIDP/00690/2020
oaire.awardNumberLA/P/0007/2020
oaire.awardTitleMountain Research Center
oaire.awardTitleMountain Research Center
oaire.awardTitleAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0007%2F2020/PT
oaire.citation.endPage11pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titlePhytomedicinept_PT
oaire.citation.volume129pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameSilva
person.familyNameAyuso
person.familyNameBarros
person.givenNameAna Rita
person.givenNameManuel
person.givenNameLillian
person.identifierABD-5857-2020
person.identifierABC-7764-2020
person.identifier469085
person.identifier.ciencia-id0E1A-35C1-C79A
person.identifier.ciencia-id6511-A057-C55F
person.identifier.ciencia-id9616-35CB-D001
person.identifier.orcid0000-0001-5819-9065
person.identifier.orcid0000-0002-1697-3857
person.identifier.orcid0000-0002-9050-5189
person.identifier.ridJ-3600-2013
person.identifier.scopus-author-id35236343600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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