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Encapsulation of clove oil in nanostructured lipid carriers from natural waxes: Preparation, characterization and in vitro evaluation of the cholinesterase enzymes

2019Journal article Open access
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dc.contributor.authorMeneses, Alessandra Cristina de
dc.contributor.authorMarques, Elisa Balbi Pinto
dc.contributor.authorLeimann, Fernanda Vitória
dc.contributor.authorGonçalves, Odinei Hess
dc.contributor.authorIneu, Rafael P.
dc.contributor.authorAraújo, Pedro Henrique Hermes de
dc.contributor.authorOliveira, Débora de
dc.contributor.authorSayer, Claudia
dc.date.accessioned2018-02-19T10:00:00Z
dc.date.accessioned2020-01-03T11:23:55Z
dc.date.available2018-01-19T10:00:00Z
dc.date.available2020-01-03T11:23:55Z
dc.date.issued2019
dc.description.abstractEugenol is a phenolic compound largely found in the clove essential oil that possesses promising biological activity. However, its low water solubility is a major concern and encapsulation is an alternative to improve water affinity. The objective of this work was to produce nanostructured lipid carriers (NLC) by hot homogenization/ultrasound emulsification and to evaluate the effect of free and encapsulated clove oil on the in vitro cholinesterase enzymes modulation using Drosophila melanogaster (DM) tissue. The NLC composed of a natural wax (carnauba or beeswax) and crodamol showed an average diameter between 121 and 367 nm with good dispersion and colloidal stability. The spherical shape and solid character together with the semi-crystalline environment confirm the formation of NLC. DSC analysis indicated polymorphic transition events of the waxes. In vitro tests using DM demonstrated that free clove oil showed a good inhibition of the butyryl and acetylcholinesterase enzymes above a concentration of 10 mM, with IC50 values of 4.3 and 3.5 mM, respectively. The dispersions of the NLC loaded with clove oil showed a decrease in the IC50 enzymes values, indicating the preservation of the clove essential oil and suggesting an increased in the solubility. Results indicate that NLC dispersions have good potential to be used for foods and cosmetic aqueous formulations possessing biological activity.
dc.description.sponsorshipAuthors thank the financial support from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), and also Laboratório Central de Microscopia Eletrônica of Universidade Federal de Santa Catarina (LCME/UFSC) for TEM analyses.
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationMeneses, Alessandra Cristina; Marques, Elisa Balbi Pinto; Leimann, Fernanda Vitória; Gonçalves, Odinei Hess; Ineu, Rafael Porto; de Araújo, Pedro Henrique Hermes; de Oliveira, Débora; Sayer, Claudia (2019). Encapsulation of clove oil in nanostructured lipid carriers from natural waxes: Preparation, characterization and in vitro evaluation of the cholinesterase enzymes. Colloids and Surfaces A: Physicochemical and Engineering Aspects. ISSN 0927-7757. 583, p. 1-8en_EN
dc.identifier.doi10.1016/j.colsurfa.2019.123879en_EN
dc.identifier.issn0927-7757
dc.identifier.urihttp://hdl.handle.net/10198/17813
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.subjectAcetylcholinesteraseen_EN
dc.subjectBeeswaxen_EN
dc.subjectButyrylcholinesteraseen_EN
dc.subjectCarnauba waxen_EN
dc.subjectClove oilen_EN
dc.subjectDrosophila melanogasteren_EN
dc.subjectEncapsulationen_EN
dc.titleEncapsulation of clove oil in nanostructured lipid carriers from natural waxes: Preparation, characterization and in vitro evaluation of the cholinesterase enzymesen_EN
dc.typejournal article
dspace.entity.typePublication
person.familyNameLeimann
person.familyNameGonçalves
person.givenNameFernanda Vitória
person.givenNameOdinei Hess
person.identifier.ciencia-id8F10-A04D-FC0D
person.identifier.orcid0000-0001-6230-9597
person.identifier.orcid0000-0002-9528-8187
person.identifier.scopus-author-id9335958400
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
relation.isAuthorOfPublicationb1102a0b-38dc-4caa-baed-ad52ccd9b230
relation.isAuthorOfPublication84c8eea0-77bb-4c18-b9d8-46f865980706
relation.isAuthorOfPublication.latestForDiscovery84c8eea0-77bb-4c18-b9d8-46f865980706

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