Determination of psychopharmaceutical adulterants in plant food supplements using UHPLC-MS/MS

Rodrigues, Manuela J.E.; Paíga, Paula; Santos, Lúcia H.M.L.M.; Amaral, Joana S.; Oliveira, Beatriz; Correia, Manuela; Delerue-Matos, Cristina

Publication

Determination of psychopharmaceutical adulterants in plant food supplements using UHPLC-MS/MS

2014Conference object

dc.contributor.author	Rodrigues, Manuela J.E.
dc.contributor.author	Paíga, Paula
dc.contributor.author	Santos, Lúcia H.M.L.M.
dc.contributor.author	Amaral, Joana S.
dc.contributor.author	Oliveira, Beatriz
dc.contributor.author	Correia, Manuela
dc.contributor.author	Delerue-Matos, Cristina
dc.date.accessioned	2018-04-04T10:44:02Z
dc.date.available	2018-04-04T10:44:02Z
dc.date.issued	2014
dc.description.abstract	In the last decades, medicinal plants and derived products have become increasingly available on the EU market as components of formulations sold as plant food supplements (PFS). Such products are legally considered as foods under the Directive 2002/46/EC [l] and do not require any kind of authorization to be placed on the market. The legal responsibility for its safety relies on the business operators. Among the several issues that may aíFect the safety of PFS, adulterations including the addition of illegal substances represent an increasing public health concern. In this context, the adulteration by the addition of psychopharmaceuticals should be investigated. Chromatographic methods, especially LC, coupled to various detectors are commonly used for the detection of fraudulent addition of pharmaceuticals in complex mixtures such as PFS. Among them, LC-MS/MS provides accurate and sensitive detectíon ofthe target compounds [2]. The purpose of this study was to assess the presence of psychopharmaceutical adulterants namely, fluoxetine, sertraline, citalopram, venlafaxine, paroxetine, trazodone, and diazepam in St. Johns wort (Hypericum perforatum) based PFS since this plant and/or extracts are traditionally used for its antidepressive properties. Analysis was performed in a Nexera Ultra-High Performance Liquid Chromatograph coupled to a triple-quadrupole mass spectrometer (LCMS-8030 Shimadzu) with an electrospray ionization source (ESI), operating in positive ion mode, using a Kinetex C18 fused core column (150 x 2.10 mm i.d.; 1.7 [im) (Phenomenex). Multiple reaction monitoring mode (MRM) was selected and pharmaceuticals were quantified by internai standard calibration method. Prior to analysis PFS samples were extracted with methanol. The methodology was applied to a set of20 St. Johns wort based PFS commercially available in the Portuguese market.	pt_PT
dc.description.sponsorship	This work received financiai support from the European Union (FEDER funds through COMPETE) and National Funds (FCT, Fundação para a Ciência e Tecnologia) through project EXPL/DTPSAP/1438/2013 and Pest-C/EQB/LA0006/2013.	pt_PT
dc.description.version	info:eu-repo/semantics/publishedVersion	pt_PT
dc.identifier.citation	Rodrigues, Manuela J.E.; Paíga, Paula; Santos, Lúcia H.M.L.M.; Amaral, J.S.; Oliveira, M.B.P.P.; Correia, Manuela; Delerue-Matos, Cristina (2014). Determination of psychopharmaceutical adulterants in plant food supplements using UHPLC-MS/MS. In XX Encontro Luso-Galego de Química. Porto	pt_PT
dc.identifier.uri	http://hdl.handle.net/10198/16667
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	pt_PT
dc.title	Determination of psychopharmaceutical adulterants in plant food supplements using UHPLC-MS/MS	pt_PT
dc.type	conference object
dspace.entity.type	Publication
oaire.awardURI	info:eu-repo/grantAgreement/FCT/COMPETE/EXPL%2FDTP-SAP%2F1438%2F2013/PT
oaire.awardURI	info:eu-repo/grantAgreement/FCT/COMPETE/PEst-C%2FEQB%2FLA0006%2F2013/PT
oaire.citation.conferencePlace	Porto, Portugal	pt_PT
oaire.citation.title	XX Encontro Luso-Galego de Química	pt_PT
oaire.fundingStream	COMPETE
oaire.fundingStream	COMPETE
person.familyName	Amaral
person.givenName	Joana S.
person.identifier.ciencia-id	5319-7DE8-BEDA
person.identifier.orcid	0000-0002-3648-7303
project.funder.identifier	http://doi.org/10.13039/501100001871
project.funder.identifier	http://doi.org/10.13039/501100001871
project.funder.name	Fundação para a Ciência e a Tecnologia
project.funder.name	Fundação para a Ciência e a Tecnologia
rcaap.rights	openAccess	pt_PT
rcaap.type	conferenceObject	pt_PT
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