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Abstract(s)
In the last decades, medicinal plants and derived products have become increasingly
available on the EU market as components of formulations sold as plant food
supplements (PFS). Such products are legally considered as foods under the Directive
2002/46/EC [l] and do not require any kind of authorization to be placed on the
market. The legal responsibility for its safety relies on the business operators.
Among the several issues that may aíFect the safety of PFS, adulterations including
the addition of illegal substances represent an increasing public health concern. In
this context, the adulteration by the addition of psychopharmaceuticals should be
investigated. Chromatographic methods, especially LC, coupled to various detectors
are commonly used for the detection of fraudulent addition of pharmaceuticals in
complex mixtures such as PFS. Among them, LC-MS/MS provides accurate and
sensitive detectíon ofthe target compounds [2].
The purpose of this study was to assess the presence of psychopharmaceutical
adulterants namely, fluoxetine, sertraline, citalopram, venlafaxine, paroxetine,
trazodone, and diazepam in St. Johns wort (Hypericum perforatum) based PFS since
this plant and/or extracts are traditionally used for its antidepressive properties.
Analysis was performed in a Nexera Ultra-High Performance Liquid Chromatograph
coupled to a triple-quadrupole mass spectrometer (LCMS-8030 Shimadzu) with an
electrospray ionization source (ESI), operating in positive ion mode, using a Kinetex
C18 fused core column (150 x 2.10 mm i.d.; 1.7 [im) (Phenomenex). Multiple reaction
monitoring mode (MRM) was selected and pharmaceuticals were quantified by
internai standard calibration method. Prior to analysis PFS samples were extracted
with methanol. The methodology was applied to a set of20 St. Johns wort based PFS
commercially available in the Portuguese market.
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Citation
Rodrigues, Manuela J.E.; Paíga, Paula; Santos, Lúcia H.M.L.M.; Amaral, J.S.; Oliveira, M.B.P.P.; Correia, Manuela; Delerue-Matos, Cristina (2014). Determination of psychopharmaceutical adulterants in plant food supplements using UHPLC-MS/MS. In XX Encontro Luso-Galego de Química. Porto