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Publication
Characterization of volatile organic compounds emitted by insect vectors of Xylella fastidiosa
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Authors
Abstract(s)
O presente estudo teve como objetivo caracterizar os compostos orgânicos voláteis (COVs) emitidos por Philaenus spumarius, principal vetor da bactéria Xylella fastidiosa, causadora de doenças graves em oliveiras, videiras e amendoeiras. Considerando a ausência de tratamentos eficazes, a identificação desses compostos pode auxiliar no desenvolvimento de armadilhas e estratégias de manejo sustentável. Os insetos foram capturados em ambientes naturais, mantidos em laboratório e submetidos à análise de COVs por cromatografia gasosa acoplada à espectrometria de massas (GC-MS A metodologia experimental foi otimizada, definindo-se as condições ideais como fibra exposta por 3 horas, utilização de 2 insetos por ensaio, o que garantiu maior confiabilidade e reprodutibilidade dos resultados. Machos isolados emitiram dois compostos, fêmeas nove, e casais apresentaram 21 compostos, evidenciando maior diversidade química na interação entre os sexos. Destacam-se o 2-etil-1-hexanol e o nonano, 2,2,4,4,6,8,8-heptametil-, presentes em todas as condições, sugerindo papel basal na comunicação química. A emissão variou sazonalmente, com maior diversidade no outono, período reprodutivo, e menor na primavera, refletindo o ciclo de vida. O estudo fornece um protocolo otimizado e identifica compostos-chave, oferecendo subsídios para futuras investigações sobre feromônios e manejo integrado de P. spumarius.
This study aimed to characterize the volatile organic compounds (VOCs) emitted by Philaenus spumarius, the main vector of the bacterium Xylella fastidiosa, which causes serious diseases in olive, grapevine, and almond trees. Given the lack of effective treatments, identifying these compounds could aid in the development of traps and sustainable management strategies. The insects were captured in natural environments, kept in the laboratory, and subjected to VOC analysis by gas chromatography-mass spectrometry (GC-MS). The experimental methodology was optimized, defining ideal conditions as fiber exposure for 3 hours, using two insects per experiment, which ensured greater reliability and reproducibility of the results. Single males emitted two compounds, females nine, and pairs emitted 21 compounds, demonstrating greater chemical diversity in the interaction between the sexes. 2-ethyl-1-hexanol and nonane, 2,2,4,4,6,8,8-heptamethyl-, stand out, present in all conditions, suggesting a basal role in chemical communication. Emission varied seasonally, with greater diversity in autumn, the reproductive period, and lower in spring, reflecting the life cycle. The study provides an optimized protocol and identifies key compounds, offering support for future research on pheromones and integrated management of P. spumarius.
This study aimed to characterize the volatile organic compounds (VOCs) emitted by Philaenus spumarius, the main vector of the bacterium Xylella fastidiosa, which causes serious diseases in olive, grapevine, and almond trees. Given the lack of effective treatments, identifying these compounds could aid in the development of traps and sustainable management strategies. The insects were captured in natural environments, kept in the laboratory, and subjected to VOC analysis by gas chromatography-mass spectrometry (GC-MS). The experimental methodology was optimized, defining ideal conditions as fiber exposure for 3 hours, using two insects per experiment, which ensured greater reliability and reproducibility of the results. Single males emitted two compounds, females nine, and pairs emitted 21 compounds, demonstrating greater chemical diversity in the interaction between the sexes. 2-ethyl-1-hexanol and nonane, 2,2,4,4,6,8,8-heptamethyl-, stand out, present in all conditions, suggesting a basal role in chemical communication. Emission varied seasonally, with greater diversity in autumn, the reproductive period, and lower in spring, reflecting the life cycle. The study provides an optimized protocol and identifies key compounds, offering support for future research on pheromones and integrated management of P. spumarius.
Description
Mestrado de dupla diplomação com a UTFPR - Universidade Tecnológica Federal do Paraná
Keywords
Philaenus spumarius Compostos orgânicos voláteis Xylella fastidiosa Feromônios