Publication
Identification of honey yeast species based on RELP analyses of the its region
| dc.contributor.author | Carvalho, Marisa | |
| dc.contributor.author | Rocha, Amélia | |
| dc.contributor.author | Estevinho, Leticia M. | |
| dc.contributor.author | Choupina, Altino | |
| dc.date.accessioned | 2011-05-19T10:24:02Z | |
| dc.date.available | 2011-05-19T10:24:02Z | |
| dc.date.issued | 2005 | |
| dc.description.abstract | In he present study, the restriction patterns generated from the region spanning the internal transcribed spacers (ITS1 and ITS2) and the 5.8S rRNA gene were used to identify a total of seven honey yeast species belonging to six different genera. Polymerase chain reaction (PCR) products of this rDNA region showed a high length variation for the different species. The size of the PCR products and the restriction patterns obtained with endonucleases HhaI, HaeIII and HinfI yielded a unique profile for each species, except for Zygoscharomyces mellis. The use of this molecular approach is proposed as a new rapid and easy method of routine honey yeast identification. En el presente estudio, el perfil de restriccin generado por la regin que abarca los separadores transcritos internos (ITS1 y ITS2) y el gen 5,8S rRNA se us para identificar un total de 7 levaduras de mieles pertenecientes a 6 gneros diferentes. Los productos de la reaccin en cadena de la polimerasa (PCR) en esta regin rDNA mostraron una alta variabilidad en la longitud para las diferentes especies. El tamao de los productos de PCR y el perfil de restriccin obtenido con endonucleasas HhaI, HaeIII y HinfI rindieron un perfil unico para cada especie, excepto para Zygoscharomyces mellis. El uso de esta aproximacion molecular se propone como un nuevo metodo rapido y facil de usar para la identificacion rutinaria de levaduras de mieles. | por |
| dc.identifier.citation | Carvalho, Marisa C.; Rocha, Amélia; Estevinho, Leticia M.; Choupina, Altino (2005) – Identification of honey yeast species based on RELP analyses of the ITS region = Identificación de especies de leveduras de miel basada en análisis RFLP de la region ITS. Ciencia y Tecnologia Alimentaria. ISSN 1135-8122. 5:1, p. 11-17 | por |
| dc.identifier.issn | 1135-8122 | |
| dc.identifier.uri | http://hdl.handle.net/10198/4399 | |
| dc.language.iso | spa | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Universidad Santiago Compostela | por |
| dc.subject | Honey yeast identification | por |
| dc.subject | RFLP | por |
| dc.title | Identification of honey yeast species based on RELP analyses of the its region | por |
| dc.title.alternative | Identificación de especies de leveduras de miel basada en análisis RFLP de la region ITS | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 17 | por |
| oaire.citation.startPage | 11 | por |
| oaire.citation.title | Ciencia y Tecnologia Alimentaria | por |
| person.familyName | Estevinho | |
| person.familyName | Choupina | |
| person.givenName | Letícia M. | |
| person.givenName | Altino | |
| person.identifier | 587972 | |
| person.identifier.ciencia-id | BA14-09D6-A406 | |
| person.identifier.ciencia-id | 1A14-77FC-9656 | |
| person.identifier.orcid | 0000-0002-9249-1948 | |
| person.identifier.orcid | 0000-0002-3956-9398 | |
| person.identifier.rid | L-5382-2014 | |
| person.identifier.scopus-author-id | 6506577664 | |
| person.identifier.scopus-author-id | 14051602500 | |
| rcaap.rights | openAccess | por |
| rcaap.type | article | por |
| relation.isAuthorOfPublication | 4a1d5ba2-1854-4ca5-89a4-73f35e964df9 | |
| relation.isAuthorOfPublication | 314b895b-a5e2-4a92-9bc6-dd708c819be4 | |
| relation.isAuthorOfPublication.latestForDiscovery | 4a1d5ba2-1854-4ca5-89a4-73f35e964df9 |