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Distribution of Alleles Linked to Pyrethroid and Amitraz Resistance in Varroa destructor across Portugal

2024Documento de conferência Acesso aberto
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dc.contributor.authorCosta, Maíra
dc.contributor.authorYadró Garcia, Carlos A.
dc.contributor.authorLopes, Ana
dc.contributor.authorQuaresma, Andreia
dc.contributor.authorRodrigues, Claúdia
dc.contributor.authorPinto, M. Alice
dc.contributor.authorHenriques, Dora
dc.date.accessioned2024-12-20T16:11:16Z
dc.date.available2024-12-20T16:11:16Z
dc.date.issued2024
dc.description.abstractVarroa destructor, an ectoparasitic mite of honey bees (Apis mellifera), is a significant threat to apiculture by causing varroosis and transmitting dangerous viruses such as the deformed wings virus. This mite can be controlled by the use of synthetic or organic acaricides. Currently, in Portugal, two classes of synthetic compounds are used: pyrethroids (fluvalinate and flumethrin), and formamidines (amitraz). However, excessive and repeated use of acaricides has led to resistance in mite populations. Resistance to pyrethroids is primarily associated with mutations in the voltage-gated sodium channel gene, and involves an amino acid change at position 925, where a leucine (L) is found in the wild type. At this position, three alleles have been described that confer Varroa resistance to pyrethroids, where leucine is replaced by valine (L925V), isoleucine (L925I), or methionine (L925M). A novel mutation at position 918 was described in a population in the Valencian Community in Spain, where the amino acid methionine, generally found at position 918, was replaced by leucine (M918L). For amitraz, resistance is linked to mutations in the Octβ2R gene, involving a substitution of asparagine by serine at position 87 (N87S), found in France, and a substitution of tyrosine by histidine at position 215 (Y215H), found in the USA. However, the distribution of these resistance-associated alleles remains unexplored in Portugal. To address this gap, we sampled Varroa mites from honey bee colonies from different locations in Portugal, and used a PCR assay with mutation-specific primers, followed by Sanger sequencing of the amplicons. The results confirmed pyrethroid resistance alleles at positions 925 and 918 in the Portuguese populations. In contrast, amitraz-resistance alleles remained undetected, although this result should be interpreted with caution as the sample size was reduced. These findings represent a step forward for understanding the status of Varroa resistance in Portugal, providing baseline data for the development of more effective, region-specific management strategies. Further research with larger sample sizes is essential to confirm these preliminary observations.pt_PT
dc.description.sponsorshipThis work was supported by the project “MITE- Varroa e vírus transmitidos: MonItorização de muTações e dEsenvolvimento de ferramentas moleculares inovadoras”funded by National Beekeeping Programme 2023-2027. FCT/MCTES (PIDDAC): CIMO, UIDB/00690/2020 (DOI: 10.54499/UIDB/00690/2020) and UIDP/00690/2020 (DOI: 10.54499/UIDP/00690/2020); and SusTEC, LA/P/0007/2020 (DOI: 10.54499/LA/P/0007/2020).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.urihttp://hdl.handle.net/10198/30762
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationMountain Research Center
dc.relationMountain Research Center
dc.relationAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectVarroa destructorpt_PT
dc.subjectAcaricides resistancept_PT
dc.titleDistribution of Alleles Linked to Pyrethroid and Amitraz Resistance in Varroa destructor across Portugalpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardNumberUIDB/00690/2020
oaire.awardNumberUIDP/00690/2020
oaire.awardNumberLA/P/0007/2020
oaire.awardTitleMountain Research Center
oaire.awardTitleMountain Research Center
oaire.awardTitleAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0007%2F2020/PT
oaire.citation.titleXX Encontro Nacional de Biologia Evolutiva: abstract bookpt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameCosta
person.familyNameYadró Garcia
person.familyNameLopes
person.familyNameQuaresma
person.familyNamePinto
person.familyNameHenriques
person.givenNameMaíra
person.givenNameCarlos A.
person.givenNameAna Rita
person.givenNameAndreia
person.givenNameM. Alice
person.givenNameDora
person.identifier.ciencia-idFB10-3DFA-D593
person.identifier.ciencia-idF71F-B08E-EC39
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person.identifier.ciencia-idF814-A1D0-8318
person.identifier.ciencia-id291F-986F-07DA
person.identifier.orcid0000-0001-6697-6581
person.identifier.orcid0000-0002-6916-3647
person.identifier.orcid0000-0002-1888-2262
person.identifier.orcid0000-0002-8678-5800
person.identifier.orcid0000-0001-9663-8399
person.identifier.orcid0000-0001-7530-682X
person.identifier.scopus-author-id57119742600
person.identifier.scopus-author-id8085507800
person.identifier.scopus-author-id55761737300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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