Logo do repositório
 
Publicação

Timolol maleate-loaded double Pickering emulsion for the treatment of glaucoma: development, characterization, and ocular biocompatibility

2026Artigo científico Acesso aberto
Ver registo simples
datacite.subject.fosCiências Médicas::Ciências da Saúde
datacite.subject.fosCiências Naturais::Ciências Químicas
datacite.subject.sdg03:Saúde de Qualidade
datacite.subject.sdg04:Educação de Qualidade
datacite.subject.sdg15:Proteger a Vida Terrestre
dc.contributor.authorTeixeira, Aniely dos Reis
dc.contributor.authorTeixeira, Liandra Gracher
dc.contributor.authorSchreiner, Tatiana B.
dc.contributor.authorSantamaria-Echart, Arantzazu
dc.contributor.authorXavier, Viviane Flores
dc.contributor.authorSilva, Gisele Rodrigues da
dc.contributor.authorBarreiro, Filomena
dc.date.accessioned2026-06-03T10:53:22Z
dc.date.available2026-06-03T10:53:22Z
dc.date.issued2026
dc.description.abstractGlaucoma is a leading cause of irreversible blindness, associated with high intraocular pressure. Topical beta-adrenergic antagonists, such as timolol maleate (TM), are first-line therapies but often have limited effectiveness due to rapid precorneal clearance and low ocular absorption. To address these limitations, a TM-loaded water-in-oil-in-water (W/O/W) double Pickering emulsion (TM-loaded DPE) was developed to enhance corneal retention, sustain drug release, and enhance ocular bioavailability. The TM-loaded DPE consisted of a primary W/O emulsion prepared from TM solution and mineral oil, stabilized by soy lecithin, combined with an external aqueous phase containing chitosan-Hydroxypropyl methylcellulose (chitosan-HPMC) particles as Pickering stabilizers, to form a W/O/W emulsion. Chitosan-HPMC particles had a size of 1.02 ± 0.16 μm, a zeta potential of 36.20 ± 2.41 mV, and a contact angle of 78.47 ± 1.80°, making them suitable Pickering stabilizers. The TM encapsulation efficiency in the TM-loaded DPE was 96%, indicating the high loading capacity of the developed system. Confocal laser scanning microscopy revealed a mononuclear droplet morphology, and the system exhibited excellent macroscopic physical stability under refrigerated conditions (4 °C, 12 months), as corroborated by the creaming index test, despite slight signs of coalescence observed in microscopic analysis. Rheological testing confirmed a non-Newtonian and shear-thinning behavior. The formulation achieved approximately 84% TM release over 48 h, indicating sustained release. The Hen egg test chorioallantoic membrane (HET-CAM) assay indicated no irritation potential, confirming its safety. Overall, these results highlight TM-loaded DPE as a promising drug delivery system for glaucoma treatment, offering improved drug retention and enhanced ocular bioavailability.eng
dc.description.sponsorshipThis research was supported by the National Council for Scientific and Technological Development (CNPq, Brazil) under grants 402092/2022-8, 309559/2021-9, and 304105/2024-4, and by the Minas Gerais State Research Support Foundation (FAPEMIG, Brazil) through grant APQ-00182-22. We also acknowledge the UFOP Multiuser Laboratory 1 of the Graduate Program in Pharmaceutical Sciences (CiPharma) for the analyses performed. Foundation for Science and Technology (FCT, Portugal) for financial support via national funds FCT/MCTES (PIDDAC) to CIMO UID/00690/2025 (10.54499/UID/00690/2025) and UID/PRR/00690/2025 (10.54499/UID/PRR/00690/2025); and SusTEC, LA/P/0007/2020 (DOI: 10.54499/LA/P/0007/2020). FCT through the institutional scientific employment program contract of A. SantamariaEchart and Liandra Gracher-Teixeira PhD research grant 2020.08803.BD (DOI: 10.54499/2020.08803.BD) is acknowledged.
dc.identifier.citationTeixeira, Aniely dos Reis; Teixeira, Liandra Gracher; Schreiner, Tatiana B.; Santamaria-Echart, Arantzazu; Xavier, Viviane Flores; Silva, Gisele Rodrigues da; Barreiro, Filomena (2026). Timolol maleate-loaded double Pickering emulsion for the treatment of glaucoma: development, characterization, and ocular biocompatibility. Journal of Drug Delivery Science and Technology. ISSN 1773-2247. 121, p. 1-12
dc.identifier.doi10.1016/j.jddst.2026.108302
dc.identifier.issn1773-2247
dc.identifier.urihttp://hdl.handle.net/10198/36832
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationMountain Research Center - UID/00690/2025
dc.relationCIMO - Mountain Research Center - UID/PRR/00690/2025
dc.relationAssociate Laboratory for Sustainability and Tecnology in Mountain Regions - LA/P/0007/2020
dc.relationCo-encapsulation of natural colorants for color and bioactivity modulation
dc.relation.ispartofJournal of Drug Delivery Science and Technology
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectDrug delivery systems
dc.subjectDouble pickering emulsion
dc.subjectChitosan
dc.subjectHydroxypropyl methylcellulose
dc.subjectTimolol maleate
dc.subjectGlaucoma treatment
dc.titleTimolol maleate-loaded double Pickering emulsion for the treatment of glaucoma: development, characterization, and ocular biocompatibilityeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberUID/00690/2025
oaire.awardNumberUID/PRR/00690/2025
oaire.awardNumberLA/P/0007/2020
oaire.awardNumber2020.08803.BD
oaire.awardTitleMountain Research Center - UID/00690/2025
oaire.awardTitleCIMO - Mountain Research Center - UID/PRR/00690/2025
oaire.awardTitleAssociate Laboratory for Sustainability and Tecnology in Mountain Regions - LA/P/0007/2020
oaire.awardTitleCo-encapsulation of natural colorants for color and bioactivity modulation
oaire.awardURIhttp://hdl.handle.net/10198/35759
oaire.awardURIhttp://hdl.handle.net/10198/36356
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0007%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//2020.08803.BD/PT
oaire.citation.endPage12
oaire.citation.startPage1
oaire.citation.titleJournal of Drug Delivery Science and Technology
oaire.citation.volume121
oaire.fundingStreamCIMO
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameTeixeira
person.familyNameSchreiner
person.familyNameBarreiro
person.givenNameLiandra Gracher
person.givenNameTatiana B.
person.givenNameFilomena
person.identifier.ciencia-id3910-3CB3-F50C
person.identifier.ciencia-idBA13-066A-678F
person.identifier.ciencia-id3418-47D5-5746
person.identifier.orcid0000-0003-1385-2037
person.identifier.orcid0000-0001-8436-9216
person.identifier.orcid0000-0002-6844-333X
person.identifier.ridL-9802-2014
person.identifier.scopus-author-id57188719717
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isAuthorOfPublication881dcc79-5c3a-4662-915a-6b1ec995e2de
relation.isAuthorOfPublication956c561b-91f6-4292-812e-8b58eaf51a19
relation.isAuthorOfPublication0391987c-ba90-488b-839a-153447ce1f0f
relation.isAuthorOfPublication.latestForDiscovery881dcc79-5c3a-4662-915a-6b1ec995e2de
relation.isProjectOfPublicationb264f2c4-92d0-4975-b1d9-0d5385dd6cbb
relation.isProjectOfPublicationf84eb641-171d-41b5-8664-4e3a31224386
relation.isProjectOfPublication6255046e-bc79-4b82-8884-8b52074b4384
relation.isProjectOfPublicationea70022d-a87d-49b7-a367-cbb2bed23eeb
relation.isProjectOfPublication.latestForDiscoveryf84eb641-171d-41b5-8664-4e3a31224386

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
1-s2.0-S1773224726003278-main.pdf
Tamanho:
9.01 MB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
1.75 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

CIMO - Artigos em Revistas Indexados à WoS/Scopus