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A microfluidic deformability assessment of pathological red blood cells flowing in a hyperbolic converging microchannel

2019Journal article Open access
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dc.contributor.authorFaustino, Vera
dc.contributor.authorRodrigues, Raquel Oliveira
dc.contributor.authorPinho, Diana
dc.contributor.authorCosta, Elísio
dc.contributor.authorSantos-Silva, Alice
dc.contributor.authorMiranda, Vasco
dc.contributor.authorAmaral, Joana S.
dc.contributor.authorLima, Rui A.
dc.date.accessioned2018-02-19T10:00:00Z
dc.date.accessioned2020-01-06T14:09:49Z
dc.date.available2018-01-19T10:00:00Z
dc.date.available2020-01-06T14:09:49Z
dc.date.issued2019
dc.description.abstractThe loss of the red blood cells (RBCs) deformability is related with many human diseases, such as malaria, hereditary spherocytosis, sickle cell disease, or renal diseases. Hence, during the last years, a variety of technologies have been proposed to gain insights into the factors a ecting the RBCs deformability and their possible direct association with several blood pathologies. In this work, we present a simple microfluidic tool that provides the assessment of motions and deformations of RBCs of end-stage kidney disease (ESKD) patients, under a well-controlled microenvironment. All of the flow studies were performed within a hyperbolic converging microchannels where single-cell deformability was assessed under a controlled homogeneous extensional flow field. By using a passive microfluidic device, RBCs passing through a hyperbolic-shaped contraction were measured by a high-speed video microscopy system, and the velocities and deformability ratios (DR) calculated. Blood samples from 27 individuals, including seven healthy controls and 20 having ESKD with or without diabetes, were analysed. The obtained data indicates that the proposed device is able to detect changes in DR of the RBCs, allowing for distinguishing the samples from the healthy controls and the patients. Overall, the deformability of ESKD patients with and without diabetes type II is lower in comparison with the RBCs from the healthy controls, with this di erence being more evident for the group of ESKD patients with diabetes. RBCs from ESKD patients without diabetes elongate on average 8% less, within the hyperbolic contraction, as compared to healthy controls; whereas, RBCs from ESKD patients with diabetes elongate on average 14% less than the healthy controls. The proposed strategy can be easily transformed into a simple and inexpensive diagnostic microfluidic system to assess blood cells deformability due to the huge progress in image processing and high-speed microvisualization technology.
dc.description.sponsorshipResearch supported by FCT with the reference projects POCI-01-0145-FEDER-016861 (PTDC/QEQFTT /4287/2014), NORTE-01-0145-FEDER-029394 (PTDC/EMD-EMD/29394/2017), NORTE-01-0145-FEDER- 030171 (PTDC/EME-SIS/30171/2017), UID/EMS/04077/2019, UID/EEA/04436/2019, UID/EMS/00532/2019, PTDC/SAU-ENB/116929/2010, by FEDER funds through the COMPETE 2020, NORTE2020, PORTUGAL2020— Programa Operacional Competitividade e Internacionalização (POCI) with the reference project POCI-01-0145-FEDER-006941 and by the NORTE-01-0145-FEDER-028178 (PTDC/EEI-EEE/28178/2017) project, funded 85% from Programa Operacional Regional do Norte and 15% from FCT. This study was also supported by FCT/MEC through national funds and cofinanced by FEDER, under the Partnership Agreement PT2020 from UCIBIO (UID/MULTI/04378/2013 -POCI/01/0145/FEDER/007728), and North Portugal Regional Coordination and Development Commission (CCDR-N)/NORTE2020/Portugal 2020 (Norte-01-0145-FEDER-000024). V.F. acknowledges the PhD scholarship SFRH/BD/99696/2014 attributed by FCT.
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationFaustino, Vera; Rodrigues, Raquel O.; Pinho, Diana; Costa, Elísio; Santos-Silva, Alice; Miranda, Vasco; Amaral, Joana S.; Lima, Rui (2019). A microfluidic deformability assessment of pathological red blood cells flowing in a hyperbolic converging microchannel. Micromachines. ISSN 2072-666X. 10, p. 1-15en_EN
dc.identifier.doi10.3390/mi10100645en_EN
dc.identifier.urihttp://hdl.handle.net/10198/20250
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.subjectBlood on chipsen_EN
dc.subjectCell deformabilityen_EN
dc.subjectChronic renal diseaseen_EN
dc.subjectDiabetesen_EN
dc.subjectHyperbolic microchannelen_EN
dc.subjectMicrofluidic devicesen_EN
dc.subjectRed blood cells (RBCs)en_EN
dc.titleA microfluidic deformability assessment of pathological red blood cells flowing in a hyperbolic converging microchannelen_EN
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04378%2F2013/PT
oaire.fundingStream5876
person.familyNamePinho
person.familyNameAmaral
person.givenNameDiana
person.givenNameJoana S.
person.identifier.ciencia-id5214-ED0C-35E8
person.identifier.ciencia-id5319-7DE8-BEDA
person.identifier.orcid0000-0002-3884-6496
person.identifier.orcid0000-0002-3648-7303
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
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