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A novel real-time PCR coupled with high resolution melting analysis as a simple and fast tool for the entomological authentication of honey by targeting Apis mellifera mitochondrial DNA

2022Artigo científico Acesso aberto
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dc.contributor.authorHonrado, Mónica
dc.contributor.authorLopes, Ana
dc.contributor.authorPinto, M. Alice
dc.contributor.authorAmaral, Joana S.
dc.date.accessioned2018-03-26T09:59:12Z
dc.date.available2018-03-26T09:59:12Z
dc.date.issued2022
dc.description.abstractHoney is one of the foods easily adulterated worldwide. Recently, the analysis of honeybee DNA has been proposed as a useful tool to authenticate the entomological origin of honey. However, the methods proposed so far require more than one polymerase chain reaction (PCR) and the use of agarose gels, making the authentication process laborious and lengthy. In this work, a novel real-time PCR coupled with high-resolution melting (HRM) analysis of a 150 bp fragment of the cytochrome c oxidase I (COI) gene is proposed as a fast and simple tool to assess honey's entomological origin by discriminating the mitochondrial DNA lineages of European honey bees (A, M and C lineages). In addition, the new tool allowed the differentiation of honeys produced by different mitotypes of C-lineage ancestry. The method showed high analytical performance and was able to successfully identify the entomological origin of honeys of known origin obtained from research apiaries/beekeepers. Therefore, it was applied to 44 commercial honeys from different countries. It confirmed the entomological authenticity of French PDO honeys that should be produced by the Corse ecotype A. m. mellifera. For the remaining honeys, the results were also in good agreement with the declared geographical origin. However, three honeys from Slovenia did not cluster with C2 mitotype A. m. carnica as expected, suggesting the mixture of honeys produced by honeybees of different mitotypes.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationHonrado, Mónica; Lopes, Ana; Pinto, M. Alice; Amaral, Joana S.. (2022). A novel real-time PCR coupled with high resolution melting analysis as a simple and fast tool for the entomological authentication of honey by targeting Apis mellifera mitochondrial DNA. Food Research International. eISSN 1873-7145. 161, p. 1-10pt_PT
dc.identifier.eissn1873-7145
dc.identifier.issn0963-9969
dc.identifier.urihttp://hdl.handle.net/10198/16532
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier
dc.subjectHoneypt_PT
dc.subjectAuthenticitypt_PT
dc.subjectHoney bee DNApt_PT
dc.subjectHRM analysis
dc.subjectApis mellifera subspecies
dc.titleA novel real-time PCR coupled with high resolution melting analysis as a simple and fast tool for the entomological authentication of honey by targeting Apis mellifera mitochondrial DNApt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleFood Research Internationalpt_PT
person.familyNameHonrado
person.familyNameLopes
person.familyNamePinto
person.familyNameAmaral
person.givenNameMónica
person.givenNameAna
person.givenNameM. Alice
person.givenNameJoana S.
person.identifier.ciencia-id4712-B40B-4B0E
person.identifier.ciencia-idF014-6835-054D
person.identifier.ciencia-idF814-A1D0-8318
person.identifier.ciencia-id5319-7DE8-BEDA
person.identifier.orcid0000-0002-5126-4693
person.identifier.orcid0000-0002-1888-2262
person.identifier.orcid0000-0001-9663-8399
person.identifier.orcid0000-0002-3648-7303
person.identifier.scopus-author-id8085507800
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication87f8840d-04b1-427a-bca9-d37eadfc0e9b
relation.isAuthorOfPublication5c8dc714-e63c-4ac6-882e-92deafa4bbe8
relation.isAuthorOfPublication0667fe04-7078-483d-9198-56d167b19bc5
relation.isAuthorOfPublication42be2cf4-adc4-4e7f-ac60-7aab515b38cd
relation.isAuthorOfPublication.latestForDiscovery0667fe04-7078-483d-9198-56d167b19bc5

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