A carregar...
Projeto de investigação
Silk Sericin as an industrial wastewater with valuable biomedical potential
Financiador
Autores
Publicações
Enhancing Wound Healing: A Comprehensive Review of Sericin and Chelidonium majus L. as Potential Dressings
Publication . Borges, Ana Margarida; Calvo, María Luisa Martín; Vaz, Josiana A.; Calhelha, Ricardo C.
Wound healing, a complex physiological process orchestrating intricate cellular and molecular
events, seeks to restore tissue integrity. The burgeoning interest in leveraging the therapeutic
potential of natural substances for advanced wound dressings is a recent phenomenon. Notably,
Sericin, a silk-derived protein, and Chelidonium majus L. (C. majus), a botanical agent, have emerged as
compelling candidates, providing a unique combination of natural elements that may revolutionize
conventional wound care approaches. Sericin, renowned for its diverse properties, displays unique
properties that accelerate the wound healing process. Simultaneously, C. majus, with its diverse
pharmacological compounds, shows promise in reducing inflammation and promoting tissue regeneration.
As the demand for innovative wound care solutions increases, understanding the therapeutic
potential of natural products becomes imperative. This review synthesizes current knowledge on
Sericin and C. majus, envisioning their future roles in advancing wound management strategies. The
exploration of these natural substances as constituents of wound dressings provides a promising
avenue for developing sustainable, effective, and biocompatible materials that could significantly
impact the field of wound healing.
Chemical characterization and bioactivities of sericin extracted from silkworm cocoons from two regions of Portugal
Publication . Reis, Sara Filipa Gomes; Vaz, Josiana A.
Along the years, sericin has been undervalued and discarded as waste from the textile silk
industry. However, recent studies have shown that sericin has great potential for
biomedical applications. The potential for medicinal applications depends on its
physicochemical properties and molecular heterogeneity. In addition, the characteristics
of sericin are influenced by its extraction method, its origin and the variety of cocoon.
This work aimed to characterize the biochemical and bioactivities of sericin from
Bragança and Castelo Branco. Sericin was extracted using the autoclave method, and its
physicochemical properties were then characterized by analyzing its amino acid content
by HPLC. In addition, its potential for antioxidant by TBARS and CAA assays, anti-
inflammatory by NO inhibition, antimicrobial by microdilution method, anti-proliferative
by SBR assay, and anticoagulant activities by APTT method was evaluated. In its pure
state, sericin did not have a high content of free amino acids, with tyrosine being identified
as the most abundant. On the other hand, when hydrolyzed sericin showed a higher
content of amino acids and serine was the most abundant. In terms of bioactivities, the
sericin tested did not show antioxidant or anti-inflammatory potential in the tests carried
out. Despite this, it showed anti-proliferative activity in contact with human tumor cell
lines and in non-tumor cell lines at a minimum concentration of 0.52 and 0.32 mg /mL,
respectively. As far as antimicrobial activity is concerned, sericin proved capable of
inhibiting the growth of the bacteria and fungi tested at concentrations between 5 and 10
mg/mL. Finally, sericin was also shown to be able to prolong the coagulation time in adult
mice plasma, presenting anticoagulant potential. The sericin from Bragança and Castelo
Branco, collected in 2019, did not differ greatly, differences in amino acid composition
were identified. In addition, the sericin collected in Bragança in 2021 and 2022, S3 and
S4, respectively, showed differences compared to the other samples and showed the best
antiproliferative, antibacterial and anticoagulant potential. Additionally, there are also
differences between extracted and commercial samples.
Unidades organizacionais
Descrição
Palavras-chave
Contribuidores
Financiadores
Entidade financiadora
Fundação para a Ciência e a Tecnologia
Programa de financiamento
3599-PPCDT
Número da atribuição
PTDC/BTA-BTA/0696/2020