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Magnetoliposomes containing calcium ferrite nanoparticles for applications in breast cancer therapy

Publication . Pereira, Daniela S.M.; Cardoso, Beatriz D.; Rodrigues, Ana Rita O.; Amorim, Carlos O.; Amaral, Vítor S.; Almeida, Bernardo G.; Queiroz, Maria João R.P.; Martinho, Olga; Baltazar, Fátima; Calhelha, Ricardo C.; Ferreira, Isabel C.F.R.; Coutinho, Paulo J.G.; Castanheira, Elisabete M.S.

Magnetoliposomes containing calcium ferrite (CaFe2O4) nanoparticles were developed and characterized for the first time. CaFe2O4 nanoparticles were covered by a lipid bilayer or entrapped in liposomes forming, respectively, solid or aqueous magnetoliposomes as nanocarriers for new antitumor drugs. The magnetic nanoparticles were characterized by UV/Visible absorption, XRD, HR-TEM, and SQUID, exhibiting sizes of 5.2 +/- 1.2 nm (from TEM) and a superparamagnetic behavior. The magnetoliposomes were characterized by DLS and TEM. The incorporation of two new potential antitumor drugs (thienopyridine derivatives) specifically active against breast cancer in these nanosystems was investigated by fluorescence emission and anisotropy. Aqueous magnetoliposomes, with hydrodynamic diameters around 130 nm, and solid magnetoliposomes with sizes of ca. 170 nm, interact with biomembranes by fusion and are able to transport the antitumor drugs with generally high encapsulation effciencies (>=70%). These fully biocompatible drug-loaded magnetoliposomes can be promising as therapeutic agents in future applications of combined breast cancer therapy.

2019Journal article

Open access

O design na valorização de territórios de montanha plataforma digital: ROOTURE residência artística: artes na montanha

Publication . Cá, Cândido; Costa, Carlos Casimiro da; Costa, Jacinta Casimiro da

A presente investigação foi desenvolvida no programa de formação em I&D Residência Artística Artes na Montanha. Teve como finalidade investigar a importância do Design na valorização da cultura e identidade de territórios de Montanha através do levantamento e análise de projetos no espaço europeu. Como resultado pretendia-se criar uma plataforma digital para arquivo destes trabalhos.

2020Conference poster

Open access

Magnetoliposomes as carriers for promising antitumor thieno[3,2-b]pyridin-7-arylamines: photophysical and biological studies

Publication . Rodrigues, Ana Rita O.; Almeida, Bernardo G.; Rodrigues, Juliana M.; Queiroz, Maria João R.P.; Calhelha, Ricardo C.; Ferreira, Isabel C.F.R.; Pires, A.; Pereira, André M.; Araújo, José Pedro; Coutinho, Paulo J.G.; Castanheira, Elisabete M.S.

Magnetoliposomes containing superparamagnetic manganese ferrite nanoparticles were tested as nanocarriers for two new promising antitumor drugs, a N-(3-methoxyphenyl)thieno[3,2-b]pyridin-7-amine (1) and a N-(2-methoxy-phenyl)thieno[3,2-b] pyridin-7-amine (2). The fluorescence emission of both compounds was studied in different polar and non-polar media, evidencing a strong intramolecular charge transfer character of the excited state of both compounds. These in vitro potent antitumor thienopyridine derivatives were successfully incorporated in both aqueous and solid magnetoliposomes, with encapsulation efficiencies higher than 75%. The magnetic properties of magnetoliposomes containing manganese ferrite nanoparticles were measured for the first time, proving a superparamagnetic behaviour. Growth inhibition assays on several human tumor cell lines showed very low GI 50 values for drug-loaded aqueous magnetoliposomes, comparing in most cell lines with the ones previously obtained using the neat compounds. These results are important for future drug delivery applications using magnetoliposomes in oncology, through a dual therapeutic approach (simultaneous chemotherapy and magnetic hyperthermia).

2017Journal article

Open access