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- Influence of pruning system and deficit irrigation on grapevine physiology, yield and grape quality of cv. Sousão (Vitis vinifera L.) growing under Mediterranean conditionsPublication . Barreales, David; Capitão, Susana dos Anjos; Monteiro, Ângela Marina Alves; Andrade, João Verdial; Ribeiro, A.C.Different strategies are currently being sought to mitigate the effects of grapevine summer water stress. Regulated Deficit Irrigation (RDI) is a strategy that has been successfully adapted. Also, some pruning systems have been identified as capable of influencing vine water balance. The aim of this work was to evaluate the effect of two RDI strategies and two pruning systems on grapevine physiology, yield and grape quality of the Sousão variety grown under Mediterranean conditions. This study was conducted in an organic vineyard in northeastern Portugal (41º31´N; 7º5´W; 326 m a.s.l.), planted in 2011 with 1103 P rootstock. The pruning systems, single Cordon and single Guyot were established in 2013. In 2019, three irrigation treatments were implemented: a full irrigation control, FI (100% ETc), and two deficit irrigations treatments, RDI25 (25% ETc) and RDI50 (50% of ETc). During the growing season, grapevine water status and physiological parameters were monitored. At harvest, yield, yield components, and grape composition were evaluated and analyzed. The results showed that the vines under an RDI regime presented significantly lower leaf water potential values than those under FI in both pruning systems. Therefore, the physiological performance was affected by decreasing its stomatal conductance, transpiration, and photosynthesis. However, the lower physiological performance did not significantly affect yield. No significant differences were observed in total soluble solids and total acidity regarding the grape composition. However, there was an increase in anthocyanins and phenolic compounds in grapes with less irrigation.