Browsing by Author "Tanamati, Ailey Aparecida Coelho"
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- Active packaging to prevent lipid oxidation on Brazil nuts (Bertholletia excelsa HBK) stored under varying temperaturesPublication . Leme, Camila M.M.; Carvalho, Amarilis Santos; Rodrigues, Vanessa de Carvalho; Santos, Adriele Rodrigues de; Tanamati, Ailey Aparecida Coelho; Gonçalves, Odinei Hess; Valderrama, Patrícia; Leimann, Fernanda VitóriaBrazil nuts are largely consumed either as a whole or as an ingredient in different food products. These seeds are rich in lipids that are susceptible to oxidation, which is the most common cause of deterioration in their sensory and nutritional quality. Active food packagings are a potential strategy to prevent food lipid oxidation. In this work, thermoplastic starch/poly (butylene adipate-co-terephthalate) (TPS/PBAT) containing water-soluble curcumin and pinhao (Araucaria angustifolia) extract as natural antioxidants were used to package Brazil nuts (Bertholletia excelsa). Packaged nuts were stored under different temperatures and analysed for up to 30 days of storage. The lipid profile of the oil extracted from the nuts before packaging showed that it is rich in unsaturated fatty acids. The UV-Vis spectra were organized into an augmented matrix and then into a principal component analysis (PCA). Results showed that for 10 & DEG;C, the control TPS/PBAT film and the film containing pinhao extract resulted in the best preservation. When evaluated at 25 & DEG;C, the nuts packed in films that contained water-soluble curcumin presented the best oxidative stability until the 15th day of storage. After this period, the oxidation reactions were significant for all samples, indicating a possible compromise on the sensory and nutritional quality of Brazil nuts.
- Avaliação da composição em ácidos gordos de folhas de urtiga (Urtica dioica)Publication . Silva, Jacqueline; Tanamati, Ailey Aparecida Coelho; Amaral, Joana S.A urtiga (Urtica dioica L.) é uma planta selvagem cujos benefícios dietéticos e terapêuticos são conhecidos desde tempos ancestrais. O seu uso na alimentação humana tem uma longa tradição, tendo sido muito utilizada pelos índios Americanos [1], estando igualmente descrita a sua utilização em diversos países Europeus [2]. Apesar do uso da urtiga na gastronomia tradicional ter caído em desuso, esta planta foi antigamente usada na confeção de sopas e outros pratos tais como omeletes, risotos, tartes e consumida como vegetal cozido [2,3]. Atualmente, em Portugal, o seu uso tem sido promovido pela Confraria da urtiga, criada em 2009, em Fornos-de-Algodres. Neste trabalho procedeu-se à determinação da composição em ácidos gordos da gordura das folhas obtida por extração em Soxhlet. Foram analisadas duas amostras colhidas em Viseu em meses diferentes (Março e Junho) e uma colhida em Março em Vila Real, em 2017. As análises dos esteres metílicos de ácidos gordos (FAMEs) foram realizadas num sistema de cromatografia gasosa com deteção de ionização em chama (GC-FID Scion 436-GC, Bruker) usando uma coluna CP-Sil 88 (50m x 0.25mm i.d, 0.20μm, Agilent J&W). A temperatura do injetor e detetor foi de 260°C e 270 ºC, respetivamente. O forno foi inicialmente colocado a 160°C por 3 min, aumentando seguidamente a 3°C/ min até 229 °C e mantendo-se durante dois minutos. Os compostos foram identificados por comparação dos tempos de retenção com uma mistura padrão de 37 FAMEs (CRM47885, Supelco). No total, foram identificados 21 ácidos gordos, sendo os compostos maioritários os ácidos α-linolénico (41,9-51,3%), linoleico (19,9-30,2%) e palmítico (9,3-14,1%). De uma forma geral, o perfil qualitativo das três amostras foi similar entre si, apresentando contudo diferenças quantitativas. Quando comparadas as amostras colhidas no mês de Março, mas em regiões geográficas distintas, verifica-se que a amostra proveniente de Viseu apresentou um teor muito superior em ácido α-linoleico e menor em ácido linoleico. Quando comparadas as amostras colhidas no mesmo local, mas em meses diferentes (Março e Junho), verifica-se a diminuição do teor de ácido α-linolénico (51.3% para 41.9%) e um aumento dos ácidos palmítico, esteárico, oleico e linoleico. Os resultados deste trabalho preliminar sugerem a influência da localização geográfica e da época de colheita, sendo contudo necessária a realização de mais estudos. Considerando os resultados obtidos, a urtiga apresenta uma gordura com um perfil interessante dado o seu elevado teor em ácido α-linolénico e cujo consumo pode ser potencialmente benéfico para a saúde.
- Bioactive and nutritional characterization of stinging nettle (Urtica dioica L.) harvested in PortugalPublication . Silva, Jacqueline; Dias, Maria Inês; Barros, Lillian; Tanamati, Ailey Aparecida Coelho; Ferreira, Isabel C.F.R.; Amaral, Joana S.Stinging nettle (Urtica dioica L.) is an edible wild plant known since ancient times for its dietary and therapeutic benefits. Despite being frequently perceived as being a weed, its use as human food has a long tradition since it was part of the ancient Greek and Roman cuisines and latter it was used as a traditional food by the native American Indians [1]. In Europe, stinging nettle has been consumed as food mainly in periods of famine and scarcity, such as wars. The leaves of this plant can be used in the confection of soups and other dishes such as omelettes, risottos, puree, tarts and consumed as cooked vegetable [2]. Although in the last decades the use of stinging nettle in gastronomy has fallen into disuse, as happen with other wild edible plants, thus it is still traditionally consumed in several regions of the world, such as the Mediterranean region, as part of a cultural and gastronomic heritage. Therefore, this study aimed to perform the nutritional, chemical and bioactive characterization of the leaves of different samples of stinging nettles harvested in Portugal. Fresh plant specimens were collected in the wild in the beginning of March 2017, from two different regions of Portugal, Viseu (40° 39′ 39″ N, 7° 54′ 34″ E) and Vila Real (41° 17′ 45″ N, 7° 44′ 46″). Another sample was collected from the same place in the region of Viseu, three months latter, in June 2017. The samples were evaluated regarding their nutritional composition including moisture, fat, proteins and ash, according to AOAC official procedures, and carbohydrates were determined by difference. Fatty acids were determined by gas chromatography coupled to a flame ionization detector (GC-FID) and phenolic compounds by High Performance Liquid Chromatography coupled to a diode-array and mass spectrometry detector using the electrospray ionization interface (HPLC-DAD-ESI/MSn). The antioxidant activity was evaluated by means of three different in vitro assays: DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, reducing power and inhibition of β-carotene bleaching. The antimicrobial susceptibility assay was performed using the Kirby-Bauer disc diffusion method against 4 Gram-positive bacteria (Bacillus cereus, Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis) and 4 Gram-negative bacteria (Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa). The leaves of stinging nettle samples presented a high percentage of moisture (78.5-83.7 g/100 g fresh leaves) with carbohydrates being the major macronutrient (47.5-50.0 g/100 g, dry basis), while fat was present in very low amounts (3.3-4.0 g/100g, dry basis). A total of 21 fatty acids were identified in the lipid fraction, with α-linolenic acid being the predominant one (41.9-51.3%). The qualitative profile among the 3 samples was identical, although quantitative differences were observed. Regarding phenolic compounds’ composition, a total of 16 compounds, including phenolic acids and flavonoids, were identified and quantified, with only 5 being present simultaneously in the 3 analyzed samples (3-O-caffeoylquinic acid, 4-O-caffeoylquinic acid, caffeic acid, isorhamnetin-3-O-rutinoside and quercetin-3-O-rutinoside). Although the sample collected in June in Viseu region was the one with lower content of phenolic compounds, it presented a similar antioxidant activity to the sample from Vila Real, which had the highest content of phenolic compounds. In general, the extracts showed a low activity towards the tested bacteria, with the exception for Pseudomonas aeruginosa, against which all three extracts showed a high activity.
- Formulation of mayonnaises containing PUFAs by the addition of microencapsulated chia seeds, pumpkin seeds and baru oilsPublication . Rojas, Valquíria Maeda; Marconi, Luís Felipe da Costa Baptista; Guimarães, Inácio Andruski; Leimann, Fernanda Vitória; Tanamati, Ailey Aparecida Coelho; Gozzo, Ângela Maria; Fuchs, Renata Hernandez Barros; Barreiro, M.F.; Barros, Lillian; Ferreira, Isabel C.F.R.; Tanamati, Ailey Aparecida Coelho; Gonçalves, Odinei HessThere is an increasing demand for healthier foodstuff containing specific compounds such as Polyunsaturated Fatty Acids (PUFAs). In the case of PUFAs, protection against oxidative degradation is challengeable and microencapsulation emerges as an alternative. Mayonnaises containing microencapsulated oils could be a source of PUFAs. The objective was to formulate mayonnaises containing microencapsulated chia seeds oil, pumpkin seeds oil or baru oil. Micrometric particles with high encapsulation efficiency were produced and thermal analyses indicated an increased thermal stability of all oils after encapsulation. Rheology studies highlighted an increase in the mayonnaise viscosity when microparticles containing chia and pumpkin seeds oil were added. Mechanical texture was not affected by the presence of microparticles in the mayonnaise in all formulations tested. Nevertheless, samples containing microcapsules up to 5%wt were not distinguished from the base-mayonnaise in the sensorial test. Overall, enriched mayonnaises were successfully produced and encapsulation was efficient in protecting oils from oxidation.
- Whey protein supplement as a source of microencapsulated PUFA-rich vegetable oilsPublication . Rojas, Valquíria Maeda; Inácio, Alexandre Guimarães; Fernandes, Isabel P.; Leimann, Fernanda Vitória; Gozzo, Ângela Maria; Fuchs, Renata Hernandez Barros; Barreiro, M.F.; Barros, Lillian; Ferreira, Isabel C.F.R.; Tanamati, Ailey Aparecida Coelho; Gonçalves, Odinei HessWhey protein supplements (WPS) intake has been increasing worldwide as they are mainly used to improve overall athletic performance. Adding other bioactives such as polyunsaturated fatty acids (PUFA) may be an alternative to help fulfill nutritional needs. Microencapsulation is able to protect PUFA-rich oils from oxidation, but important aspects of particle production and their influence on food properties must be evaluated. This study aimed to develop WPS with microencapsulated green coffee and walnut oils using stearic acid as a wall material. Oxidative stability (differential scanning calorimetry) of the oils increased (from 82 ± 4 to 110 ± 10 kJ mol−1 for green coffee oil and from 90 ± 5 to 149 ± 1 kJ mol−1 for walnut oil) after encapsulation and WPS rheological properties were not affected by the microcapsules (p 0.05). Sensory analysis of the supplement containing microencapsulated green coffee oil showed a lower sensory preference than the blank sample, but no difference was found with the blank sample in the case of walnut oil (p 0.05). The encapsulation strategy used to produce an enriched WPS was efficient in protecting the oils from oxidative degradation.