Browsing by Author "Sousa, Isabel Maria dos Santos"
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- Contamination levels that compromise the hygienic condition in the handling of foodPublication . Sousa, Isabel Maria dos Santos; Gonçalves, Marília; Portelinha, Rita; Ferreira, Tânia; Afonso, Andrea Luísa Fernandes; Nogueira, António José M.; Pires, BrunoMicrobiological contamination of food is a major public health problem, affecting millions of people worldwide. Most of the contamination is associated with bad practices in the handling and processing of food, due to poor hygiene of food worker´s hands or utensils and surfaces used in this manipulation. This study evaluates the hygienic condition of food handling in the district of Bragança, determining microbial contamination of utensils and worker´s hands, during the period 2009-2011. With respect to the worker´s hands, 39 % of cases showed contamination. In 57 % of these samples total coliforms were identified, in 26% of them fecal coliforms, Staphylococcus aureus in 10% and finally 7% with Escherichia coli. The utensils contamination was confirmed in 30% of samples with results "not satisfactory" and 4 % had results "bad". These contaminations stand out 60 % of the samples with the presence of total germs, 28 % with total coliforms, fecal coliforms with 9 % and even 3 % with Escherichia coli. It was also found that only 37 % of the samples developed positively. It was concluded that there is a better cleaning of utensils manipulation than the worker´s hands, however the trend to improve the hygienic condition is most pronounced in the hands of these workers. The implementation of a program of rules for good hygiene practices will reduce these contaminants to lower levels.
- Contamination levels that compromise the hygienic condition in the handling of foodPublication . Sousa, Isabel Maria dos Santos; Gonçalves, Marília; Portelinha, Rita; Ferreira, Tânia; Afonso, Andrea Luísa Fernandes; Nogueira, António José M.; Pires, BrunoMicrobiological contamination of food is a major public health problem, affecting millions of people worldwide. Most of the contamination is associated with bad practices in the handling and processing of food, due to poor hygiene of food worker´s hands or utensils and surfaces used in this manipulation. This study evaluates the hygienic condition of food handling in the district of Bragança, determining microbial contamination of utensils and worker´s hands, during the period 2009-2011. With respect to the worker´s hands, 39 % of cases showed contamination. In 57 % of these samples total coliforms were identified, in 26% of them fecal coliforms, Staphylococcus aureus in 10% and finally 7% with Escherichia coli. The utensils contamination was confirmed in 30% of samples with results "not satisfactory" and 4 % had results "bad". These contaminations stand out 60 % of the samples with the presence of total germs, 28 % with total coliforms, fecal coliforms with 9 % and even 3 % with Escherichia coli. It was also found that only 37 % of the samples developed positively. It was concluded that there is a better cleaning of utensils manipulation than the worker´s hands, however the trend to improve the hygienic condition is most pronounced in the hands of these workers. The implementation of a program of rules for good hygiene practices will reduce these contaminants to lower levels.
- Incidência de anemia ferripriva numa população pediátrica detectada na rotina pré-operatóriaPublication . Sousa, Isabel Maria dos Santos; Costa, Elísio; Ferreira, Amélia; Cota, Susana; Barbot, José; Iglésias, IsabelO despiste de ferropenia pré operatório numa população pediátrica, pode constituir uma oportunidade para detectar e corrigir estadios deficitários de ferro, susceptíveis de provocar sequelas a médio/longo prazo. Com o objectivo de detectar estas situações, fomos avaliar sistematicamente em todos os hemogramas, que deram entrada no Serviço de Patologia Clínica do Hospital Maria Pia num período de 6 meses, a presença de indicadores de défice de ferro, nomeadamente: hemoglobina (Hb), volume globular médio e RDW (red cell distribution width). Nos casos em que foi encontrada alteração em algum destes parâmetros foi efectuado: protoporfirina eritrocitária livre (PEL), ferro, ferritina e transferrina. Nas situações que confirmaram a presença de microcitose sem défice de ferro foi efectuado o doseamento de Hb A2 e Hb F e pesquisa de inclusões de Hb H. Das 557 amostras que deram entrada para estudo de rotina pré-operatória, nove (1,62%) apresentaram alteração de pelo menos um dos parâmetros considerados para despiste de ferropenia. Sete destas amostras revelaram ferropenia, duas das quais associadas a anemia e as outras duas apresentavam a pesquisa de inclusões de Hb H positivas, sugestivo do diagnóstico de α-talassemia minor. Com o presente trabalho pensamos ter contribuído, a diferentes níveis, para o aproveitamento integral de todas as informações analíticas fornecida pelos modernos contadores hematológicos.