Browsing by Author "Silva, Fabiane de Lima"
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- Antioxidant, photoprotective and inhibitory activity of tyrosinase in extracts of Dalbergia ecastaphyllumPublication . Morais, Daniel Vieira; Costa, Maria Angélica Carvalho; Bárbara, Marivalda; Silva, Fabiane de Lima; Moreira, Manuela M.; Delerue-Matos, Cristina; Dias, L.G.; Estevinho, Leticia M.; Carvalho, Carlos Alfredo Lopes deDalbergia ecastaphyllum is a native Brazil plant with importance for beekeeping, and widely used in folk medicine. For the first time, the extracts of this plant were assessed for the presence of hydrophilic and lipophilic antioxidants, as well as inhibition of tyrosinase, free radicals scavenging and sunscreen protection. The antioxidant activity was evaluated by free radical scavenging (DPPH) andβ-carotene bleaching assay. The tyrosinase inhibitory activity was evaluated and calculated the EC50. The photoprotective activity was measured using different concentrations of D. ecastaphyllum extracts. The Sun Protection Factor (SPF) of the samples was higher than 6, and the sample from Ilhe ´us showed the most pronounced photoprotective effect. Sample from Canavieiras presented the highest antioxidant activity by free radical scavenging DPPH andβ-carotene bleaching method, with 92.41% and 48.34%, respectively. All samples inhibited the tyrosinase, especially the sample from Prado that was most effective (124.62μg.mL-1). Significant negative correlation was found between flavonoid contents and inhibition of tyrosinase. The overall results provide relevant information about the Dalbergia ecastaphyllum species, indicating as potential material to cosmetic and pharmaceutical industry.
- Botanical origin, microbiological quality and physicochemical composition of the Melipona scutellaris pot-pollen (“samburá”) from Bahia (Brazil) RegionPublication . Oliveira, Daiane de Jesus; Santos, Daiane Rodrigues dos; Andrade, Brunelle Ramos; Nascimento, Andreia Santos do; Silva, Macela Oliveira da; Mercês, Carize da Cruz; Lucas, Cátia Ionara Santos; Silva, Samira Maria Peixoto Cavalcante; Carvalho, Paula Dib de; Silva, Fabiane de Lima; Estevinho, Leticia M.; Carvalho, Carlos Alfredo Lopes deMelipona scutellaris Latreille, 1811 is of economic importance for local beekeepers, besides its relevance in the pollination of native plant species of the Brazilian Atlantic forest. Currently, data on the use of floral resources by Meliponini colonies are scarce, particularly in urban environments. We evaluated the botanical origin, the microbiological and physicochemical characteristics of pollen stored by M. scutellaris in colonies in an urban environment. The samples (n = 44) were obtained from the metropolitan region of Salvador, Bahia, Brazil, a region of intense urban and industrial activities. We identified 52 pollen types belonging to 21 botanical families. The botanical families Fabaceae, Myrtaceae, and Anacardiaceae represented most pollen types. Aerobic psychrotrophic bacteria, Bacillus spp., molds and yeasts, fecal coliforms, Escherichia coli, Staphylococcus aureus, sulfite-reducing Clostridium, and Salmonella spp. were not found in the samples. We identified and quantified fatty acids with carbon numbers varying from C4 to C20. For the physicochemical parameters, the following variations were verified: moisture (47.3% to 55.70%), ash (3.45% to 5.90%), protein (10.19 to 24.02%), pH (3.28 to 3.99), acidity (237.20 to 557.10 meq/kg), lipids (2.43 to 7.94%), carbohydrates (10.85 to 28.89%) and total energy value (170.60 to 216.99 kcal/100g). Pollen stored (“samburá”) by bees is a complete food and a source of nutrients with therapeutic potential. Pollen stored by M. scutellaris consists of a heterofloral pollen with physicochemical and microbiological qualities, considered safe for human consumption. Moreover, it contains linoleic and linolenic essential fatty acids making it a potential nutraceutical product.
- Dalbergia ecastaphyllum leaf extracts: in vitro inhibitory against enzymes related to metabolic syndrome, inflammation and neurodegenerative diseasesPublication . Morais, Daniel Vieira; Moreira, Manuela M.; Silva, Fabiane de Lima; Costa, Maria Angélica Carvalho; Delerue-Matos, Cristina; Carvalho, Carlos Alfredo Lopes de; Estevinho, Leticia M.For the first time, the anti-hemolytic activity and the enzyme inhibitory activities of Dalbergia ecastaphyllum leaves extracts were tested against α-amylase, α-glucosidase, lipase, acetylcholinesterase, butyrylcholinesterase, tyrosinase and hyaluronidase. The phenolic profile of the obtained extracts was also investigated by high-performance liquid chromatography with photodiode array detection (HPLC-PAD). The extracts showed inhibitory activity against all enzymes evaluated, with the highest inhibitory activity reported for the enzyme hyaluronidase (28.28 ± 2.43 to 72.19 ± 1.40 μg mL-1). The obtained extracts also demonstrate anti-hemolytic activity (52.22 ± 1.62 to 71.17 ± 1.82%). Among the phenolic compounds identified, protocatechuic, vanillic and β-resorcylic acids were the most abundant (1.13 ± 0.06 to 2.53 ± 0.06, 0.90 ± 0.06 to 2.19 ± 0.06 and 1.03 ± 1.62 to 22.11 ± 1.62 mg L-1, respectively). In the statistical analysis, a significant correlation was found between the flavonoids content and all enzymes inhibitory activities. The present study showed that D. ecastaphyllum leaves extracts may have the potential to be used in the therapeutic treatment of several diseases such as Alzheimer, Parkinson, type 2 diabetes mellitus, hyperglycemia, and pigmentation, as well as those associated with oxidative stress.
- Phytochemical study and antioxidant activity of Dalbergia ecastaphyllumPublication . Lucas, Cátia Ionara Santos; Ferreira, Adailton Freitas; Costa, Maria Angélica Carvalho; Silva, Fabiane de Lima; Estevinho, Leticia M.; Carvalho, Carlos Alfredo Lopes deThe chemical profile of Dalbergia ecastaphyllum has been indicated as the botanical origin of Brazilian red propolis, an apicultural product with proven therapeutic properties. However, few studies have investigated this plant species. This study evaluated and compared microbiological quality, chemical composition, and antioxidant activity of stem and leaf samples of D. ecastaphyllum. The samples were collected in February 2015, in the southern region of the state of Bahia, Brazil. We performed the microbiological analyses, determined the contents of fatty acid, total phenol and flavonoid, and identified the chemical profile and antioxidant activit. Escherichia coli, Salmonella spp. and sulfite reducing clostridial spores were not detected in the samples. Acids of the family ω3 were recorded in the stems and ω6 in the leaves. The leaves presented better nutritional quality of the fraction, better antioxidant capacity in the tests by the DPPH method and β-carotene bleaching. There were 49 chemical compounds, of which 38 belonged to the class of flavonoids. The results indicate that stems and leaves of D. ecastaphyllum have biological properties. Leaves particularly are better for functional food formulation and as natural antioxidant.
- Use of the electronic tongue as a tool for the characterization of Melipona scutellaris Latreille honeyPublication . Nascimento, Andreia Santos do; Silva, Fabiane de Lima; Machado, Cerilene Santiago; Silva, Samira Maria Peixoto Cavalcante; Estevinho, Leticia M.; Dias, L.G.; Carvalho, Carlos Alfredo Lopes deThis study aimed to characterize honey of Melipona scutellaris regarding its physicochemical parameters using the electronic tongue (e-tongue) technique combined with the multivariate statistical analysis for honey differentiation. Physicochemical parameters were evaluated following official methods of chemical analyses. A potentiometric electronic tongue with 16 cross-sensitivity sensors was used for the analysis. The Principal Component Analysis and the Cluster Analysis distinguished two groups for the sample set in the evaluation of physicochemical parameters, similar to results found using the electronic tongue. We verified a correlation greater than 0.70 between the profile of potentiometric signals and values of pH, ashes, electrical conductivity, HMF, diastase activity, reducing sugars, and apparent sucrose. The combined use of the electronic tongue with the statistical analysis showed the similarity between samples through the formation of two groups of the sample set. The electronic tongue may be used as a complement to traditional techniques of analyses to determine honey physicochemical parameters, constituting a promising tool in association with the multivariate statistical analysis.