Percorrer por autor "Sghaier, Iheb"
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- Characterization of portuguese monofloral honeys based on physicochemical parameters and free amino acids profilePublication . Sghaier, Iheb; Falcão, Soraia; Vilas-Boas, Miguel; Mokkadem, YassinHoney is produced by honey bees from the nectar of plants as well as honeydew resulting from exudations of plants or excretions of plant-sucking insects. The composition depends mainly on the phyto-geographical conditions, climate and honeybee species involved in its production. According to the botanical origin, honey can be classified as monofloral or multifloral, if resulting predominantly from a single or from several plant species, respectively. Nowadays, monofloral honeys are most demanded by consumers and are sold at a higher price, thanks to their unique characteristics such as flavour, taste and biological properties. The valuing increment also promotes the susceptible to fraudulent practices by mislabelling with regard to the botanical or geographical origin or by mixing with lower cost and low-quality honeys. Amino acid analysis can be used as a tool for botanical and/or geographical origin discrimination in honey authenticity. Its presence in honey is derived from the bees and from the plants visited by the bees, considering that the pollen is the most important source of proteins and the bees the source of free amino acids. The aim of this work will be to discriminate selected monofloral Portuguese honeys, such as Erica, Lavandula, Castanea, Eucaliptus, as well as honeydew, through LC-MS without previous derivatization. Honey characterization was carried out through the identification of their floral origin by pollen analysis and by the evaluation of the physicochemical and biochemical parameters. Also, the composition of amino acids, phenolic compounds and antioxidant activity was evaluated.