Browsing by Author "Peron, Ana Paula"
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- Analytical validation of an ultraviolet–visible procedure for determining vitamin D3 in vitamin D3-loaded microparticles and toxigenetic studies for incorporation into foodPublication . Silva, Tamires Barlati Vieira; Oliveira, Anielle; Moreira, Thaysa Fernandes Moya; Silva, Kelly Cristina; Zanin, Rodolfo Campos; Bona, Evandro; Gonçalves, Odinei Hess; Shirai, Marianne Ayumi; Peron, Ana Paula; Leimann, Fernanda VitóriaVitamin D is a water-insoluble compound presented in two main forms (D2 and D3), susceptible to environmental conditions. Microencapsulation is an alternative to supplements and preserve vitamin D properties in foods. Entrapment efficiency (EE) is the main property to evaluate the encapsulation effectiveness and therefore it is of interest the study of analytical methods for the identification and quantification of this compound within the particle. This paper describes a low cost UV–Vis methodology validation to the identification and quantification of vitamin D3 in microparticles produced by hot homogenization. The method was validated following the International Conference on Harmonization (ICH) guidelines. To guarantee safe application in foodstuff, microparticles toxigenicity was evaluated with Allium cepa L. in vivo model, showing no cytotoxic nor genotoxic potential. High entrapment efficiency was obtained, the results also demonstrated that the concentration of vitamin D3 in microparticles can be safely accessed by the validated method.
- Evaluation of berberine nanoparticles as a strategy to modulate acetylcholinesterase activityPublication . Leimann, Fernanda Vitória; Souza, Luma Borges de; Oliveira, Byanca Pereira Moreira de; Rossi, Bruna Franzon; Silva, Patricia Sabino da; Shiraishi, Carlos Seiti Hurtado; Kaplum, Vanessa; Abreu, Rui M.V.; Pereira, Carla; Barros, Lillian; Peron, Ana Paula; Ineu, Rafael Porto; Oechsler, Bruno Francisco; Sayer, Claudia; Araujo, Pedro Henrique Hermes de; Gonçalves, Odinei Hess; Shiraishi, Carlos S.H.Researchers have concentrated efforts in the search for natural-based reversible inhibitors for cholinesterase enzymes as they may play a key role in the treatment of degenerative diseases. Diverse plant alkaloids can inhibit the action of acetylcholinesterase and, among them, berberine is a promising bioactive. However, berberine has poor water solubility and low bioavailability, which makes it difficult to use in treatment. The solid dispersion technique can improve the water affinity of hydrophobic substances, but berberine solid dispersions have not been extensively studied. Safety testing is also essential to ensure that the berberine-loaded solid dispersions are safe for use. This study investigated the effectiveness of berberine-loaded solid dispersions (SD) as inhibitors of acetylcholinesterase enzyme (AChE). Docking simulation was used to investigate the influence of berberine on AChE, and in vitro assays were conducted to confirm the enzymatic kinetics of AChE in the presence of berberine. Berberine SD also showed improved cytotoxic effects on tumoral cells when dispersed in aqueous media. In vivo assays using Allium cepa were implemented, and no cytotoxicity/genotoxicity was found for the berberine solid dispersion. These results suggest that berberine SD could be a significant step towards safe nanostructures for use in the treatment of neurodegenerative diseases.
- Extraction of bioactive compounds from Curcuma longa L. using deep eutectic solvents: in vitro and in vivo biological activitiesPublication . Oliveira, Grazielle; Marques, Caroline; Oliveira, Anielle; Santos, Amanda de Almeida; Amaral, Wanderlei; Ineu, Rafael P.; Leimann, Fernanda Vitória; Peron, Ana Paula; Igarashi-Mafra, Luciana; Mafra, Marcos R.In this work, deep eutectic solvents (DES-based menthol and cholinium chloride) and the ethanol, temperature, and times were selected to extract bioactive compounds from the rhizome, leaves, and flowers Curcuma longa L., using ultrasound-assisted extraction. Analyzes antioxidant, flavonoids, antimicrobial, chelation Fe2+, inhibition of the cholinesterase's enzymes, cytotoxicity, and genotoxicity in Allium cepa cells were performed. The extracts showed results of iron chelation and antibacterial. Curcuma flowers and leaves' extracts inhibited food spoilage bacteria with values above 45%, with substantial iron-chelating activity above 50%. Extracts obtained by DES based on menthol and lactic acid exhibited a high percentage of inhibition of acetyl and butyryl cholinesterase. In contrast, flower extracts obtained by menthol and acetic acid showed low inhibition of cholinesterase enzyme activity. No extract showed cytotoxicity and genotoxicity. Biological activities showed a high potential for the application of these extracts in the food and pharmaceutical industries.
- In vitro and in vivo evaluation of enzymatic and antioxidant activity, cytotoxicity and genotoxicity of curcumin-loaded solid dispersionsPublication . Silva, Igor; Peron, Ana Paula; Leimann, Fernanda Vitória; Bressan, Getúlio Nicola; Krum, Bárbara Nunes; Fachinetto, Roselei; Pinela, José; Calhelha, Ricardo C.; Barreiro, M.F.; Ferreira, Isabel C.F.R.; Gonçalves, Odinei Hess; Ineu, Rafael P.Curcumin, the main bioactive polyphenolic compound in Curcuma longa L. rhizomes has a wide range of bioactive properties. Curcumin presents low solubility in water and thus limited bioavailability, which decreases its applicability. In this study, cytotoxic effects of curcumin solid dispersions (CurSD) were evaluated against tumor (breast adenocarcinoma and lung, cervical and hepatocellular carcinoma) and non-tumor (PLP2) cells, while cytotoxic and genotoxic effects were evaluated in Allium cepa. The effect of the CurSD on the acetylcholinesterase (AChE), butyrylcholinesterase (BChE), glutathione S-transferase (GST), andmonoamine oxidase (MAO A-B) enzymes was determined, as well as its capacity to inhibit the oxidative hemolysis (OxHLIA) and the formation of thiobarbituric acid reactive substances (TBARS). CurSD are constituted by nanoparticles that are readily dispersible in water, and inhibited 24% and 64% of the AChE and BChE activity at 100μM, respectively. GST activity was inhibited at 30μM while MAO-A and B activity were inhibited at 100μM. CurSD showed cytotoxicity against all the tested tumor cell lines without toxic effects for non-tumor cells. No cytotoxic and genotoxic potential was detected with the Allium cepa test.CurSD maintained the characteristics of free curcumin on the in vitro modulation of important enzymes without appreciable toxicity.