Browsing by Author "Perez Vazquez, Ana"
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- Biological properties of Hypericum sampsonii and Hypericum japonica: comparison of the antioxidant, anti-inflammatory and antimicrobial activitiesPublication . Barciela Alvarez, Paula; Perez Vazquez, Ana; Rodrigues, Daniele Bobrowski; Silva, Tayse Ferreira; Mandim, Filipa; Pires, Tânia C.S.; Carpena Rodríguez, María; Simal-Gandara, Jesus; Barros, Lillian; Prieto Lage, Miguel A.Hypericum was famous in ancient times for its properties, morphology, and adaptability. Currently, it has naturalized in practically all parts of the world from America to Australia. It is considered a cosmopolitan species present on all five continents. Hypericum is the largest genus of the Hypericaceae family (more than 500 species classified in 36 taxonomic sections). The study of these ethnomedicinal plants has been developed mainly and traditionally in China where there are more than 64 species, of which 19 have been related to the presence of phytochemicals with anti-tumor, anti-inflammatory, antimicrobial, antidepressant, analgesic, or neuroprotective properties, among others [1]. Remarkably, the great potential of these species has hardly been scientifically evaluated [2]. In this study, the comparison and study of the different biological properties of the species Hypericum japonica and Hypericum sampsonii was carried out. Cellular antioxidant activity was evaluated with murine macrophage cells, with both species showing 77±5% inhibition at a maximum tested concentration of 2,000 μg/mL. Antimicrobial activity was evaluated on both food contaminants and clinical bacteria and fungi. MIC and MBC were determined by colorimetric assay, where 10 μL of liquid was added from each well that showed no color change was plated on solid medium, blood agar (7% sheep blood) and incubated at 37°C for 24 h, respectively. Ampicillin and Steptomicin were used for all bacteria tested and Methicillin was also used for Staphylococcus aureus. The MIC results showed that H. japonica has a higher antimicrobial effect on Gram-negative bacteria and in Enterococcus faecalis. While H. sampsonii showed a more effective power for the rest of Gram-positive tested bacteria. The antifungal activity was performed applying the method described by Heleno et. al., 2013, using Aspergillus brasiliensis and A. fumigatus and using Ketoconazole as positive control, showing no activity for both species [3]. Anti-inflammatory activity was studied using six cell lines (CaCo2, MCF-7, NCl-460, PLP2, RAW 264.7) and Ellipticine as a control in all but RAW 264.7 where Dexamethasone was used. For all cell lines, both Hypericum species showed lower GI50 than the control, being H. japonica the one with higher anti-inflammatory effect of the two extract samples. This study is the first to provide more information on the bioactivities of two less-analyzed Hypericum species
- Study of the antioxidant, antimicrobial and anti-inflammatory activities of two Euphorbia speciesPublication . Perez Vazquez, Ana; Alvarez, Paula Barciela; Silva, Tayse Ferreira; Rodrigues, Daniele Bobrowski; Mandim, Filipa; Pires, Tânia C.S.; Carpena Rodríguez, María; Simal-Gandara, Jesus; Barros, Lillian; Prieto Lage, Miguel A.In this research, a study of the antioxidant, antimicrobial and anti-inflammatory activities of two Euphorbia species (Euphorbia hirta and Euphorbia jolkinii) was developed. Euphorbia species belong to the Euphorbiaceae family, being commonly found in America and tropical Africa. Euphorbia species have been traditionally used in folk medicine to treat gonorrhea, migraines, intestinal parasites, warts, and skin diseases [1]. Since there is more data available of Euphorbia hirta, the aim of this study was to provide a comparison between E. hirta and E. jokinii regarding their antioxidant, antimicrobial and anti-inflammatory activities. For the cellular antioxidant activity, murine macrophage cells RAW 264.7 were used following the procedure described by Wolf & Lui (2007). E. hirta showed better inhibition percentage results than E. jolkinii, being 2,000 μg/mL, the maximum concentration tested for both samples. Extracts of both Euphorbia species were used to determine their antimicrobial activity. Gram-negative and Gram-positive food and clinical bacteria were tested for this assay. Moreover, Streptomycin, Methicillin and Ampicillin were used as controllers to compare both MIC and MBC results. For the food bacteria, E. jolkinii had better MIC results than E. hirta when Gram-negative bacteria were tested, although it was lower than controllers. MBC results for both Euphorbia species were similar and lower than the controllers. When Gram-positive clinical bacteria were tested, similar MIC results between both extracts were obtained. However, when L. monocytogenes and S. aureus were tested, E. jolkinii showed better MIC results. Antifungal activity was also assessed using ketoconazole as control and Aspergillus brasiliensis and Aspergillus fumigatus as fungi. Unsatisfactory results were obtained for both Euphorbia species for both fungi. AGS, CaCo2, MCF-7, NCl-H460, PLP2 and RAW 264.7 cell lines were used for the anti-inflammatory assay. Ellipticine was used as control in all the cell lines except in RAW 264.7, where dexamethasone was used. Results showed lower anti-inflammatory activity of the 2 extracts compared to the controllers in all cell lines, being E. jolkinii the one with lower GI50. Considering the obtained results, although E. hirta has been widely studied, E. jolkinii showed better antimicrobial and anti-inflammatory activities, being a suitable option for the pharmacological industry.