Browsing by Author "Palmeira, Carlos M."
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- Antioxidant capacity and toxicological evaluation of pterospartum tridentatum flower extractsPublication . Ferreira, Fernanda M.; Dinis, Lia Tânia; Azedo, Pedro; Galhano, Cristina; Cardoso, Susana M.; Domingues, Maria R.M.; Pereira, Olívia R.; Simões, Anabela; Palmeira, Carlos M.; Peixoto, Francisco P.Pterospartum tridentatum Willk. (prickled broom) is an autochthonous plant, common in Portuguese territory. The yellow flowers are used in traditional medicine, as a potential cure for all body illnesses, mainly for throat irritation treatment or for diabetes, hypertension and hypercholesterolemia therapy.Despite its traditional use, no toxicological assessment has been performed as we know. A high antioxidant activity of P. tridentatum flower water extract was acessed in good agreement with its ESI-MS spectrum that revealed the presence of several flavonoids, as luteolin-O-(O-acetyl)-glucuronide, luteolin-O- glucuronide or isorhamnetin-O-hexoside. Mitocondrial respiratory rates (state 4, state 3 and FCCP-stimulated respiration) and respiratory indexes (respiratory control and P/O ratios) showed no consistent decrease of respiratory and phosphorylative efficiencies for the concentrations tested (up to 500 μg.mL-1). Cytotoxicity evaluation, using MTT assay, was reliable with the previous results. In conclusion, for the concentration range commonly used P. tridentatum flowers usage can be regarded as harmless and trustworthy.
- Effect of a pterospartum tridentatum enriched phenolic extract in mitochondrial bioenergetic functionsPublication . Ferreira, Fernanda M.; Peixoto, Francisco P.; Pereira, Olívia R.; Dinis, Lia Tânia; Domingues, Maria R.M.; Crespí, António; Palmeira, Carlos M.; Cardoso, Susana M.Pterospartum tridentatum Willk. is an autochthonous and common plant in Portugal, locally known as ‘carqueija’. Leaves and stems are normally used in cooking, to flavour rice, roast meat or hunting animals and leaves are also used as condiment in fresh salads. Despite its traditional use, no evaluation in mitochondrial bioenergetics has been performed. P. tridentatum leaf extract phenolic composition was elucidated by electrospray ionization-mass spectrometry (ESI-MS). M. aquatica extract effects were evaluated over mitochondrial membrane potential by using a Clarktype oxygen electrode in the presence of succinate as respiratory substrate. Cytotoxicological effects of plant extracts were evaluated on HepG2 cells throughout MTT assay. The ESI-MS spectrum of P. tridentatum leaves aqueous extract revealed the presence of several luteolin and isorhamnetin derived phenolic compounds, which can be associated to the health benefits claimed for this plant species. Yet, P. tridentatum leaves extract (up to 100 lg plant extract mg protein _1) stimulated state 4 and FCCP-stimulated liver mitochondria respiratory rates and inhibited the state 3 respiratory rate. Hence, respiratory control ratios were diminished, indicating a phosphorylative efficiency decrease, due to inner mitochondrial membrane permeability induced by P. tridentatum leaves extract. Nevertheless, P/O ratios were not affected in the range of concentrations tested and cytotoxicity evaluation using MTT assay (50 and 125 ug plant extract) showed no significant decrease over HepG2 cell viability. This dual behaviour reflects the P. tridentatum hormetic effects over mitochondrial activity. Conclusion: Overall, the present study suggests that consumption of P. tridentatum leaves should be regarded as safe and present benefits concerning the modern lifestyles.
- Effect of pterospartum tridentatum enriched phenolic extract in mitochondrial bioenergetic functionsPublication . Ferreira, Fernanda M.; Peixoto, Francisco P.; Pereira, Olívia R.; Dinis, Lia Tânia; Domingues, Maria R.M.; Crespí, António; Palmeira, Carlos M.; Cardoso, Susana M.Pterospartum tridentatum Willk. is an autochthonous and common plant in Portugal, locally known as ‘carqueija’. Leaves and stems are normally used in cooking, to flavour rice, roast meat or hunting animals and leaves are also used as condiment in fresh salads. Despite its traditional use, no evaluation in mitochondrial bioenergetics has been performed. P. tridentatum leaf extract phenolic composition was elucidated by electrospray ionization-mass spectrometry (ESI-MS). M. aquatica extract effects were evaluated over mitochondrial membrane potential by using a Clarktype oxygen electrode in the presence of succinate as respiratory substrate. Cytotoxicological effects of plant extracts were evaluated on HepG2 cells throughout MTT assay. The ESI-MS spectrum of P. tridentatum leaves aqueous extract revealed the presence of several luteolin and isorhamnetin derived phenolic compounds, which can be associated to the health benefits claimed for this plant species. Yet, P. tridentatum leaves extract (up to 100 lg plant extract mg protein _1) stimulated state 4 and FCCP-stimulated liver mitochondria respiratory rates and inhibited the state 3 respiratory rate. Hence, respiratory control ratios were diminished, indicating a phosphorylative efficiency decrease, due to inner mitochondrial membrane permeability induced by P. tridentatum leaves extract. Nevertheless, P/O ratios were not affected in the range of concentrations tested and cytotoxicity evaluation using MTT assay (50 and 125 ug plant extract) showed no significant decrease over HepG2 cell viability. This dual behaviour reflects the P. tridentatum hormetic effects over mitochondrial activity. Conclusion: Overall, the present study suggests that consumption of P. tridentatum leaves should be regarded as safe and present benefits concerning the modern lifestyles.
- Polyphenols characterization and toxicological evaluation of pterospartum tridentatum leaf extractsPublication . Ferreira, Fernanda M.; Peixoto, Francisco P.; Pereira, Olívia R.; Dinis, Lia Tânia; Domingues, Maria R.M.; Palmeira, Carlos M.; Cardoso, Susana M.Pterospartum tridentatum Willk. (prickled broom) is an autochthonous and common plant in Portugal. Leaves and stems are normally used in cooking, to flavour rice, roast meat or hunting animals. Leaves are also used as a condiment in fresh salads and, despite of its traditional use, no toxicological evaluation has been performed. P. tridentatum leaves aqueous extract ESI-MS spectrum revealed the presence of several luteolin and isorhamnetin derived phenolic compounds, which can be associated to the health benefits claimed for this plant species. Still, P. tridentatum leaves extract (up to 100 µg plant extract.mg-1 protein) stimulated state 4 and FCCP-stimulated liver mitochondria respiratory rates and inhibited the state 3 respiratory rate. Respiratory control ratio was decreased, indicating a dysfunction in respiratory activity induced by P. tridentatum leaves extract and, in good agreement with the previous results, cytotoxicity evaluation by MTT assay (50 and 125 µg plant extract.10-6 cells) showed a decrease on HepG2 cell viability. Overall, the present study suggests that the consumption of P. tridentatum leaves in high amounts or continuously should be regarded as noxious.
- Polyphenols characterization and toxicological evaluation of pterospartum tridentatum leaf extractsPublication . Ferreira, Fernanda M.; Peixoto, Francisco P.; Pereira, Olívia R.; Dinis, Lia Tânia; Domingues, Maria R.M.; Palmeira, Carlos M.; Cardoso, Susana M.Pterospartum tridentatum Willk. (prickled broom) is an autochthonous and common plant in Portugal. Leaves and stems are normally used in cooking, to flavour rice, roast meat or hunting animals. Leaves are also used as a condiment in fresh salads and, despite of its traditional use, no toxicological evaluation has been performed. P. tridentatum leaves aqueous extract ESI-MS spectrum revealed the presence of several luteolin and isorhamnetin derived phenolic compounds, which can be associated to the health benefits claimed for this plant species. Still, P. tridentatum leaves extract (up to 100 µg plant extract.mg-1 protein) stimulated state 4 and FCCP-stimulated liver mitochondria respiratory rates and inhibited the state 3 respiratory rate. Respiratory control ratio was decreased, indicating a dysfunction in respiratory activity induced by P. tridentatum leaves extract and, in good agreement with the previous results, cytotoxicity evaluation by MTT assay (50 and 125 µg plant extract.10-6 cells) showed a decrease on HepG2 cell viability. Overall, the present study suggests that the consumption of P. tridentatum leaves in high amounts or continuously should be regarded as noxious.
- Polyphenols characterization and toxicological evaluation of pterospartum tridentatum leaf water extractsPublication . Ferreira, Fernanda M.; Peixoto, Francisco P.; Pereira, Olívia R.; Dinis, Lia Tânia; Domingues, Maria R.M.; Palmeira, Carlos M.; Cardoso, Susana M.Pterospartum tridentatum Willk. (prickled broom) is an autochthonous and common plant in Portugal. Leaves and stems are normally used in cooking, to flavour rice, roast meat or hunting animals. Leaves are also used as a condiment in fresh salads and, despite of its traditional use, no toxicological evaluation has been performed. P. tridentatum leaves aqueous extract ESI-MS spectrum revealed the presence of several luteolin and isorhamnetin derived phenolic compounds, which can be associated to the health benefits claimed for this plant species. Still, P. tridentatum leaves extract (up to 100 µg plant extract.mg-1 protein) stimulated state 4 and FCCP-stimulated liver mitochondria respiratory rates and inhibited the state 3 respiratory rate. Respiratory control ratio was diminished, indicating a decrease in phosphorylative efficiency due to inner mitochondrial membrane induced by P. tridentatum leaves extract. Nevertheless, previous results, cytotoxicity evaluation by MTT assay (50 and 125 µg plant extract) showed no significant decrease on HepG2 cell viability. Overall, the present study suggests that the consumption of P. tridentatum leaves should be regarded as safe.
- Polyphenols characterization and toxicological evaluation of pterospartum tridentatum leaf water extractsPublication . Ferreira, Fernanda M.; Peixoto, Francisco P.; Pereira, Olívia R.; Dinis, Lia Tânia; Domingues, Maria R.M.; Palmeira, Carlos M.; Cardoso, Susana M.Pterospartum tridentatum Willk. (prickled broom) is an autochthonous and common plant in Portugal. Leaves and stems are normally used in cooking, to flavour rice, roast meat or hunting animals. Leaves are also used as a condiment in fresh salads and, despite of its traditional use, no toxicological evaluation has been performed. P. tridentatum leaves aqueous extract ESI-MS spectrum revealed the presence of several luteolin and isorhamnetin derived phenolic compounds, which can be associated to the health benefits claimed for this plant species. Still, P. tridentatum leaves extract (up to 100 µg plant extract.mg-1 protein) stimulated state 4 and FCCP-stimulated liver mitochondria respiratory rates and inhibited the state 3 respiratory rate. Respiratory control ratio was diminished, indicating a decrease in phosphorylative efficiency due to inner mitochondrial membrane induced by P. tridentatum leaves extract. Nevertheless, previous results, cytotoxicity evaluation by MTT assay (50 and 125 µg plant extract) showed no significant decrease on HepG2 cell viability. Overall, the present study suggests that the consumption of P. tridentatum leaves should be regarded as safe.
- Toxicological evaluation and polyphenols characterization of pterospartum tridentatum leaf extractsPublication . Ferreira, Fernanda M.; Peixoto, Francisco P.; Pereira, Olívia R.; Dinis, Lia Tânia; Domingues, Maria R.M.; Palmeira, Carlos M.; Cardoso, Susana M.Pterospartum tridentatum Willk. (prickled broom) is a common autochthonous plant in Portugal. Leaves and stems are normally used in cooking, to flavour rice, roast meat or hunting animals. Leaves are also used as a condiment in fresh salads and, despite of its traditional use, no toxicological evaluation has been performed. P. tridentatum leaves aqueous extract ESI-MS spectrum revealed the presence of several luteolin and isorhamnetin derived phenolic compounds, which can be associated to the health benefits claimed for this plant species. Still, P. tridentatum leaves extract (up to 100 µg plant extract.mg-1 protein) stimulated state 4 and FCCP-stimulated liver mitochondria respiratory rates and inhibited the state 3 respiratory rate. Respiratory control ratio was decreased, indicating a dysfunction in respiratory activity induced by P. tridentatum leaves extract and, in good agreement with the previous results cytotoxicity evaluation by MTT assay (50 and 125 µg plant extract.10-6 cells) showed a decrease on HepG2 cell viability. Overall, the present study suggests that the consumption of P. tridentatum leaves in high amounts or continuously should be regarded as potencially noxious.
- Toxicological evaluation and polyphenols characterization of pterospartum tridentatum leaf extractsPublication . Ferreira, Fernanda M.; Peixoto, Francisco P.; Pereira, Olívia R.; Dinis, Lia Tânia; Domingues, Maria R.M.; Palmeira, Carlos M.; Cardoso, Susana M.Pterospartum tridentatum Willk. (prickled broom) is a common autochthonous plant in Portugal. Leaves and stems are normally used in cooking, to flavour rice, roast meat or hunting animals. Leaves are also used as a condiment in fresh salads and, despite of its traditional use, no toxicological evaluation has been performed. P. tridentatum leaves aqueous extract ESI-MS spectrum revealed the presence of several luteolin and isorhamnetin derived phenolic compounds, which can be associated to the health benefits claimed for this plant species. Still, P. tridentatum leaves extract (up to 100 µg plant extract.mg-1 protein) stimulated state 4 and FCCP-stimulated liver mitochondria respiratory rates and inhibited the state 3 respiratory rate. Respiratory control ratio was decreased, indicating a dysfunction in respiratory activity induced by P. tridentatum leaves extract and, in good agreement with the previous results cytotoxicity evaluation by MTT assay (50 and 125 µg plant extract.10-6 cells) showed a decrease on HepG2 cell viability. Overall, the present study suggests that the consumption of P. tridentatum leaves in high amounts or continuously should be regarded as potencially noxious.