Percorrer por autor "Mediavilla, Irene"
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- Assessment of the use of common juniper (Juniperus communis L.) foliage following the cascade principlePublication . Mediavilla, Irene; Bados, Raquel; Barros, Lillian; Xavier, Virginie; Finimundy, Tiane C.; Pires, Tânia C.S.P.; Heleno, Sandrina A.; Calhelha, Ricardo C.; Amaral, Joana S.; Rizzo, Andrea Maria; Casini, David; Lombardi, Giacomo; Chiaramonti, David; Camara, Miguel; Suarez, Ana; Ardid, Tomas; Esteban Pascual, Luis SaulJuniperus communis L. is a species commonly grown in regions of the Northern Hemisphere, and is a good candidate to be cultivated in marginal lands. Plants coming from a pruning performed in a natural population located in Spain were used to assess the yield and quality of different products obtained following the cascade principle. A total of 1050 kg of foliage biomass were crushed, steam-distilled, and separated into fractions to produce biochar and absorbents for the pet industry using pilot plants. The obtained products were analysed. The essential oil, with a yield of 0.45% dry basis and a qualitative chemical composition similar to that described for the berries in international standards or monographs, showed antioxidant activity with promising CAA results (inhibition of 89% of the cell's oxidation). However, regarding antibacterial and antifungal activities, it only inhibited the growth of microorganisms at the maximum concentration tested, 2.5%. Concerning the hydrolate, it did not show bioactivity. Regarding the biochar, whose yield was 28.79% dry basis, interesting results were obtained for its characterisation as a possible soil improver for agronomic purposes (PFC 3(A)). Finally, promising results were obtained regarding the use of common juniper as absorbent, taking into account the physical characterisation and odour control capacity.
- Assessment of the use of common juniper (Juniperus communis L.) foliage following the cascade principle: essential oil, absorbents and biocharPublication . Mediavilla, Irene; Bados, Raquel; Barros, Lillian; Xavier, Virginie; Finimundy, Tiane C.; Amaral, Joana S.; Rizzo, Andrea Maria; Casini, David; Lombardi, Giacomo; Cámara, Miguel; Suárez, Ana; Ardid, TomásThe main objective of the BeonNAT project is to create added-value bio-based products by growing underused tree and shrub species in marginal land. One of the species selected within the project is juniper (Juniperus communis L). Foliage biomass coming from a natural population in Spain was selected and a first sampling of wild biomass (1000 kg fresh plants with stem diameters below 50 mm) was performed. This biomass was distilled and, after it, different alternatives were defined in the project to obtain bioproducts from the distilled biomass following the cascade concept. Among them, absorbents for the pet industry and biochar have been selected in this work. Concerning the steam distillation, an essential oil yield of 0.44% (w/w d.b) was obtained and the characterisation of the essential oil compounds showed that it was close to meet the limits considered by the ISO 8897:2010. A fraction of the distilled biomass was used to produce pellets, whose quality as absorbents for the pet industry was promising. Finally, the rest of the distilled biomass was used to obtain biochar with a yield of 28.79 % (w/w d.b) and characteristics which indicated a good degree of carbonisation and stability.
- Chemical and bioactive characterization of the essential oils obtained from three mediterranean plantsPublication . Xavier, Virginie; Finimundy, Tiane C.; Heleno, Sandrina A.; Amaral, Joana S.; Calhelha, Ricardo C.; Vaz, Josiana A.; Pires, Tânia C.S.; Mediavilla, Irene; Esteban Pascual, Luis Saul; Ferreira, Isabel C.F.R.; Barros, LillianCupressus sempervirens L., Juniperus communis L. and Cistus ladanifer L. are Mediterranean arboreal and shrub species that possess essential oils (EO) in their leaves and branches. This study aimed at characterizing the EOs obtained by steam distillation from the three species collected in different locations from Spain (Almazán, Andévalo, Barriomartín, Cerezal, Ermitas and Huéscar). For this purpose, volatiles composition was determined by GC-MS, and different bioactivities were evaluated. The highest content in terpenes was observed in C. sempervirens (Huéscar origin) followed by J. communis (Almazán origin), corresponding to 92% and 91.9% of total compounds, respectively. With exception of C. ladanifer from Cerezal that presented viridiflorol as the most abundant compound, all the three species presented in common the -pinene as the major compound. The EOs from C. ladanifer showed high antibacterial potential, presenting MIC values from 0.3 to 1.25 mg/mL. Concerning other bioactivities, C. ladanifer EO revealed an oxidation inhibition of 83%, while J. communis showed cytotoxicity in the MCF-7 cell line, and C. sempervirens and C. ladanifer EOs exhibited the highest potential on NCI-H460 cell lines. Nevertheless, some EOs revealed toxicity against non-tumoral cells but generally presented a GI50 value higher than that of the tumor cell lines.
- Yield, chemical composition and bioactivity of essential oils from common juniper (Juniperus communis L.) from different spanish originsPublication . Esteban Pascual, Luis Saul; Mediavilla, Irene; Xavier, Virginie; Amaral, Joana S.; Pires, Tânia C.S.P.; Calhelha, Ricardo C.; Lopez, Cesar; Barros, LillianEssential oils (EOs) obtained from Juniperus communis L. are frequently used in the production of bioproducts. However, there are no studies regarding industrial crops' production, allowing for better control of the quality and production of juniper EOs. To select the plant material for developing future crops of this species in northern Spain, four locations where this shrub species grows in the wild were selected and samples of both genera were collected. The EOs were obtained by steam distillation, and their chemical composition and bioactivity were evaluated. The results showed that the yield of EOs from male and female samples were within the usual reported ranges, varying between 0.24 and 0.58% (dry basis, d.b.). However, limonene content in three locations varied between 15 and 25%, which is between 100% and 200% higher than the values usually reported for other European countries. The antibacterial activity was determined by broth microdilution and showed that gram-positive bacteria were more susceptible to the tested EOs since, in general, lower MIC values were obtained compared to gram-negatives. The EOs from location 1 (L1F) and 2 (L2M) inhibited the growth of six out of the eight clinical strains tested. Samples from location 1 were particularly effective, exhibiting MBC against two gram-negative (E. coli and P. mirabilis) and one gram-positive bacteria (E. faecalis). Moreover, the majority of the EOs tested showed anti-inflammatory activity. Cytotoxic effect has been demonstrated in tumor cell lines, with the best results observed against gastric carcinoma (AGS) cells (GI(50) between 7 to 77 mu g/mL). Although generally presenting higher GI50, most samples also inhibited the growth of non-tumoral cells, particularly hepatocytes (PLP2 cells). Therefore, its use for their anti-proliferative activity should consider specific conditions to avoid damaging normal cells. Finally, the results and conclusions obtained led to the selection of the female shrubs from location 1 (L1F) as the plant material to be propagated in order to produce plants for a future juniper crop.