Browsing by Author "Machado, Cerilene Santiago"
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- Antioxidants activity and physicochemical properties of honey from social bees of the Brazilian semiarid regionPublication . Silva, Irana Paim; Caldas, Maiara Janine Machado; Machado, Cerilene Santiago; Nascimento, Andreia Santos do; Lordêlo, Maurício Santana; Bárbara, Marivalda; Evangelista-Barreto, Norma Suely; Estevinho, Leticia M.; Carvalho, Carlos Alfredo Lopes de; Silva I.P.This study compared the nutraceutical potential of Apis mellifera and Melipona quadrifasciata anthidioides honeys from the semiarid region of Bahia, Brazil, using microbiological, melissopalynological, and physicochemical techniques. Total phenols and flavonoids and the antioxidant activity were also determined. All samples had good microbiological quality and safety with an absence of coliforms, Escherichia coli, Salmonella spp., Staphylococcus coagulase positive and Clostridium sulphite reducing spores. Were identified 41 pollen types belonging to 23 botanical families. Myrtaceae, Anacardiaceae, and Sapindaceae were the predominant pollen types in A. mellifera honey, while honey Malvaceae was the most representative in the M. q. anthidioides. Regarding the physicochemical parameters evaluated, 75% complied with the standards established by the Brazilian and European quality legislation. The honey samples of A. mellifera had higher values of phenolic compounds and flavonoids (260.0 to 341.51 mgGAE.kg−1 and from 114.44 to 216.29 mgQE.kg−1, respectively). The samples from M. q. anthidioides presented higher antioxidant activity. The honeys of A. mellifera and M. q. anthidioides from the semiarid regions presented distinct botanical compositions, suggesting that both species use different plant sources, which possibly influenced the parameters related to honey quality as well as the content of phenolic compounds.
- Detection of biogenic amines in mead of social beePublication . Silva, Irana Paim; Dias, L.G.; Silva, Macela Oliveira da; Machado, Cerilene Santiago; Paula, Vanessa B.; Evangelista-Barreto, Norma Suely; Carvalho, Carlos Alfredo Lopes de; Estevinho, Leticia M.Mead is an alcoholic beverage, resulting from the fermentation of honey and water, although it is an old drink, some fermentation products are still unknown. Biogenic amines have driven research on food and beverages due to their toxicity and capacity to cause damage to the human health. This study aimed to evaluate the presence of biogenic amines in mead from honey of two species of social bees, using different fining agents (bentonite, banana peel flour, and passion fruit peel flour) and storage in oak barrel for 120 days. We observed the presence of three biogenic amines for each type of mead. Putrescine was reported only in samples of mead of Apis mellifera. Tryptamine and histamine were not detected, while tyramine was observed in the mead samples of honey from Melipona quadrifasciata anthidioides. Samples clarified with fruit peels increased the number of amines. In the analysis of the histamine, used as standard, obtained limits of detection and quantification of 2.47 mg/L and of 7.48 mg/L, respectively. In this study, the amines observed remained below the limit of detection, and did not pose a risk to consumers. The finings used demonstrated a profile similar to the control mead, and its use was satisfactory.
- Microbiological assessment, nutritional characterization and phenolic compounds of bee pollen from Mellipona mandacaia Smith, 1983Publication . Santa Bárbara, Marivalda F.; Machado, Cerilene Santiago; Sodré, Geni da Silva; Dias, L.G.; Estevinho, Leticia M.; Carvalho, Carlos Alfredo Lopes deThis study aims to assess the microbiological parameters and the chemical composition of 21 samples of stingless bee pollen (Melipona mandacaia) from two regions of Bahia, Brazil (João Dourado and Uibaí), with particular emphasis on the nutritional value, total phenols and flavonoids and fatty acids composition. Regarding the microbiological quality, the studied microorganisms (moulds and yeasts, coliforms, Escherichia coli, Staphyl ococcus aureus, Salmonella sp., psychrotrophic and sulfite-reducing Clostridia) were absent in all samples. On the other hand, the values obtained for the aerobic mesophilic microorganism ranged from 11.0 ± 1.0 to 1.32 ± 1.2 cfu·g < sup = -1 < /sup = (JD samples) and from 282 ± 3.82 to 688 ± 10.1 cfu·g < sup > -1 < /sup > (U samples). The nutritional parameters (moisture, ash, water activity, pH, total acidity, protein, fiber, total phenolic, flavonoids and reducing sugars) were within the stipulated by law, except for pH and moisture content, which presented superior and inferior values, respectively. Polyunsaturated fatty acids (54.1%) were significantly higher than saturated (42.18%) and monounsaturated (3.71%). It was found that the bee pollen is safe from the microbiological point of view and has a good nutritional quality. The influence of the geographical origin on the assessed parameters was evident, especially concerning the fatty acid profile.
- Storage methods, phenolic composition, and bioactive properties of Apis mellifera and Trigona spinipes pollenPublication . Santa Bárbara, Marivalda F.; Moreira, Manuela M.; Machado, Cerilene Santiago; Chambó, Emerson Dechechi; Pascoal, Ananias; Carvalho, Carlos Alfredo Lopes de; Sodré, Geni da Silva; Delerue-Matos, Cristina; Estevinho, Leticia M.The aim of this study was to evaluate the influence of lyophilization and drying in stove on phenolic compounds content and the biological activity of Apis mellifera and Trigona spinipes pollens produced in Brazil. In general, the bee pollen produced by T. spinipes presented highest antioxidant activity in dried and fresh samples assessed either by test of plasma ferric reduction capacity (FRAP) and free radical scavenging assay (DPPH) methods. For A. mellifera bee pollen the antioxidant activity was higher on the fresh samples. Nevertheless, b-carotene bleaching assay (BCB) and linoleic acid content were higher in T. spinipes samples, mainly in the fresh ones. Higher antioxidant activity was owing to higher content in phenolic compounds. Lyophilization method was the best for phenolic compounds’ conservation for both species. The bee pollen of both species has a high amount of flavonoids: kaempferol-3-O-glucoside was the most abundant in A. mellifera while for T. spinipes the most prevalent was resorcylic acidþepicatechin. All extracts presented antibacterial activity against Saphylococcus aureus (ATCC 43300)TM, (ESA 83138150), (ESA 32), Pseudomonas aeruginosa (ATCC 15442)TM, Pseudomonas aeruginosa (MRC.4) and (MRC.10). The methods used for storage influenced the biological properties of bee pollen from both species. Regarding the content of phenolic compounds, differences were observed amongst the pollen types: for A. mellifera these were best preserved with lyophilization, while for T. spinipes the three storage methods were equivalent.
- Use of the electronic tongue as a tool for the characterization of Melipona scutellaris Latreille honeyPublication . Nascimento, Andreia Santos do; Silva, Fabiane de Lima; Machado, Cerilene Santiago; Silva, Samira Maria Peixoto Cavalcante; Estevinho, Leticia M.; Dias, L.G.; Carvalho, Carlos Alfredo Lopes deThis study aimed to characterize honey of Melipona scutellaris regarding its physicochemical parameters using the electronic tongue (e-tongue) technique combined with the multivariate statistical analysis for honey differentiation. Physicochemical parameters were evaluated following official methods of chemical analyses. A potentiometric electronic tongue with 16 cross-sensitivity sensors was used for the analysis. The Principal Component Analysis and the Cluster Analysis distinguished two groups for the sample set in the evaluation of physicochemical parameters, similar to results found using the electronic tongue. We verified a correlation greater than 0.70 between the profile of potentiometric signals and values of pH, ashes, electrical conductivity, HMF, diastase activity, reducing sugars, and apparent sucrose. The combined use of the electronic tongue with the statistical analysis showed the similarity between samples through the formation of two groups of the sample set. The electronic tongue may be used as a complement to traditional techniques of analyses to determine honey physicochemical parameters, constituting a promising tool in association with the multivariate statistical analysis.