Advisor(s)
Abstract(s)
The aim of this study was to evaluate the influence of lyophilization and drying in stove on phenolic compounds content
and the biological activity of Apis mellifera and Trigona spinipes pollens produced in Brazil. In general, the bee pollen produced
by T. spinipes presented highest antioxidant activity in dried and fresh samples assessed either by test of plasma
ferric reduction capacity (FRAP) and free radical scavenging assay (DPPH) methods. For A. mellifera bee pollen the antioxidant
activity was higher on the fresh samples. Nevertheless, b-carotene bleaching assay (BCB) and linoleic acid content
were higher in T. spinipes samples, mainly in the fresh ones. Higher antioxidant activity was owing to higher
content in phenolic compounds. Lyophilization method was the best for phenolic compounds’ conservation for both
species. The bee pollen of both species has a high amount of flavonoids: kaempferol-3-O-glucoside was the most abundant
in A. mellifera while for T. spinipes the most prevalent was resorcylic acidþepicatechin. All extracts presented antibacterial
activity against Saphylococcus aureus (ATCC 43300)TM, (ESA 83138150), (ESA 32), Pseudomonas aeruginosa
(ATCC 15442)TM, Pseudomonas aeruginosa (MRC.4) and (MRC.10). The methods used for storage influenced the biological
properties of bee pollen from both species. Regarding the content of phenolic compounds, differences were
observed amongst the pollen types: for A. mellifera these were best preserved with lyophilization, while for T. spinipes
the three storage methods were equivalent.
Description
Keywords
Honey HPLC-PDA Pseudomonas aeruginosa Pseudomonas spp Staphylococcus aureus
Pedagogical Context
Citation
Santa Bárbara, Marivalda F.; Moreira, Manuela M.; Machado, Cerilene S.; Chambó, Emerson D.; Pascoal, Ananias; de Carvalho, Carlos Alfredo L.; da Silva Sodré, Geni; Delerue-Matos, Cristina; Estevinho, Leticia M. (2020). Storage methods, phenolic composition, and bioactive properties of Apis mellifera and Trigona spinipes pollen. Journal of Apicultural Research. ISSN 0021-8839. p. 1-10