Browsing by Author "Kowalczyk, Alicja"
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- Correlation of polymorphism of AQP7 gene with the sperm cells quality traits of the polish holstein friesian bullsPublication . Kowalczyk, Alicja; Kowalewska-Łuczak, Inga; Czerniawska-Piątkowska, Ewa; Cantalapiedra, Jesús J.; Cerqueira, J.O.L.; Araújo, José PedroThe purpose of this study was to evaluate whether single nucleotide substitutions within exon 4 of the quality of AQP7 gene may affect the sperm cells of Polish Holstein-Friesian bulls. The research material was biological material (semen and blood) collected from 60 Holstein-Friesian bulls of the same age. The nucleotide substitutions analyzed are located in exon 4 of the AQP7 gene and are responsible for missense mutations. Individual genotypes were determined using the PCR-RFLP method. Evaluation of sperm quality in terms of concentration, volume, motility, and sperm viability was performed using photometry, flow cytometry, and a computer assisted sperm analyzer. The above studies indicate that all four nucleotide substitutions analyzed in exon 4 of AQP7 gene affect the individual quality characteristics of the analyzed semen of Holstein-Friesian bulls.
- Relationship between milk protein polymorphism and selected cows’ reproductive indicesPublication . Czerniawska-Piątkowska, Ewa; Cioch-Szklarz, Barbara; Kowalczyk, Alicja; Wrzecińska, Marcjanna; Wójcik, Jerzy; Kordan, Władysław; Araújo, José Pedro; Cerqueira, Joaquim; Kossakowski, Kamil; Cwynar, Przemysław; Sablik, PiotrThis research sought to determine the effect of selected milk protein polymorphisms on the reproduction parameters of 598 black and white Polish Holstein–Friesian cattle. The analyzed genes were kappa-casein (CSN3) and beta-lactoglobulin (BLG). The following reproductive indexes were selected: the age at first calving, the interpregnancy period, the calving interval, and the insemination index. The influence of the identified genotypes on the course of parturition in cows was also analyzed. Source data were collected for each cow from the beginning of the herd life and reproduction to its culling from the herd or the end of its third lactation. Data on the age at first calving, the amount of semen portions for artificial insemination (insemination index), the interpregnancy period, and the calving interval for each cow were also collected. A contingency analysis was performed through contingency tables using a Pearson’s chi-squared test for each CSN3 and BLG genotype. The results show that the BB genotype of the kappa-casein gene was associated with the most favorable values of reproductive indicators. In the case of the calving interval, the values were significantly more favorable than those of other genotypes (p 0.05). No effect of beta-lactoglobulin polymorphism on the analyzed reproductive indices was observed. On the other hand, in the case of the interpregnancy period, significant statistical differences were obtained between the AA and BB genotypes. The analyzed kappa-casein and beta-lactoglobulin genotypes did not significantly influence the course of parturition in cows. To conclude, the genotype polymorphism BB CSN3 is the most favorable for the performance of the cows in the examined herd.